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Growth inhibition and formation of morphologically abnormal cells of Akashiwo sanguinea (Hirasaka) G. Hansen et Moestrup by cell contact with Cochlodinium polykrikoides Margalef

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Abstract

We investigated the growth interaction between Cochlodinium polykrikoides and Akashiwo sanguinea using bi-algal cultures. When cultured together, morphologically abnormal cells of A. sanguinea appeared and the growth of A. sanguinea notably decreased. When C. polykrikoides and A. sanguinea were cultured separately in inner and outer wells separated by a membrane with a pore size of 3 μm, neither species showed suppressed growth and no morphologically abnormal cells of A. sanguinea were observed. Furthermore, filtrates from C. polykrikoides cultures did not affect the cell morphology or the growth of A. sanguinea. When the abnormal cells of A. sanguinea were transferred from bi-algal cultures into fresh medium and cultured as a mono-algal culture, cells recovered their normal morphology and multiplied. Therefore, the growth inhibition and formation of morphologically abnormal cells of A. sanguinea would be induced by constant cell contact with C. polykrikoides.

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References

  • Du Q, Huang Y, Wang X (1993) Toxic dinoflagellate red tide by a Cochlodinium sp. along the coast of Fujian, China. In: Smayda TJ, Shimizu Y (eds) Toxic phytoplankton blooms in the sea. Elsevier, New York, pp 235–238

    Google Scholar 

  • Goldman JC, Dennett MR, Riley CB (1981) Test for allelopathic interactions between two marine microalgal species grown in intensive cultures. Curr Microbiol 6:275–279

    Article  Google Scholar 

  • Goldman JC, Riley CB, Dennett MR (1982) The effect of pH in intensive microalgal cultures II. Species competition. J Exp Mar Biol Ecol 57:15–24

    Article  Google Scholar 

  • Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds) Cultures of marine invertebrate animals. Plenum Press, New York, pp 29–60

    Google Scholar 

  • Jeong HJ, Yoo YD, Kim JS, Kim TH, Kim JH, Kang NS, Yih W (2004) Mixotrophy in the phototrophic harmful alga Cochlodinium polykrikoides (Dinophycean): prey species, the effects of prey concentration, and grazing impact. J Eukaryot Microbiol 51:563–569

    Article  PubMed  Google Scholar 

  • Kim HG (1998) Harmful algal blooms in Korean coastal waters focused on three fish-killing dinoflagellates. In: Kim HG, Lee SG, Lee CK (eds) Harmful algal blooms in Korea and China. National Fisheries Research and Development Institute, Pusan, pp 1–20

    Google Scholar 

  • Kim DI, Matsuyama Y, Nagasoe S, Yamaguchi M, Yoon YH, Oshima Y, Imada N, Honjo T (2004) Effects of temperature, salinity and irradiance on the growth of the harmful red tide dinoflagellate Cochlodinium polykrikoides Margalef (Dinophyceae). J Plankton Res 26:61–66

    Article  Google Scholar 

  • Kim CJ, Kim HG, Kim CH, Oh HM (2007) Life cycle of the ichthyotoxic dinoflagellate Cochlodinium polykrikoides in Korean coastal waters. Harmful Algae 6:104–111

    Article  Google Scholar 

  • Kumada K, Takeda K, Aramaki T (1980) III Yatsushiro Kaiiki, Yatsushiro Kai-2. In: Fisheries Agency, Fukuokaken Suisan Shikenjyou, Sagaken Suisan Shikenjyou, Nagasaki Suisan Shikenjyou, Kumamoto Suisan Shikenjyou, Kagoshima Suisan Shikenjyou (eds) Kyushu Seiganiki Akashiwo Yosatsu Chosa Houkokusho, pp 125–136 (in Japanese)

  • Lim WA, Jung CS, Lee CK, Cho YC, Lee SG, Kim HG, Chung IK (2002) The outbreak, maintenance, and decline of the red tide dominated by Cochlodinium polykrikoides in the coastal waters off southern Korea from August to October, 2000. J Korean Soc Oceanogr 7:68–77

    Google Scholar 

  • Lundholm N, Hansen PJ, Kotaki Y (2005) Lack of allelopathic effects of the domoic acid-producing marine diatom Pseudo-nitzschia multiseries. Mar Ecol Prog Ser 288:21–33

    Google Scholar 

  • Margalef R (1961) Hidrografía y fitoplankton de un área marina de la costa meridional de Puerto Rico. Invest Pesq 18:33–96 (in Spanish)

    Google Scholar 

  • Marshall HG (1995) Succession of dinoflagellate blooms in the Chesapeake Bay, USA. In: Lassus P et al (ed) Harmful algal blooms. Intercept Ltd, Andover, pp 615–620

    Google Scholar 

  • Matsuoka K, Iwataki M (2004) Present status in study on a harmful unarmored dinoflagellate Cochlodinium polykrikoides Margalef. Bull Plankton Soc Japan 51:38–45 (in Japanese)

  • Matsubara T, Nagasoe S, Yamasaki Y, Shikata T, Shimasaki Y, Oshima Y, Honjo T (2007) Effects of temperature, salinity, and irradiance on the growth of the dinoflagellate Akashiwo sanguinea. J Exp Mar Biol Ecol 342:226–230

    Article  Google Scholar 

  • Ogston AG (1970) In: Balazs EA (ed) Chemistry and molecular biology of the intercellular matrix. Academic, New York, pp 1231–1240

  • Schmidt LE, Hansen PJ (2001) Allelopathy in the prymnesiophyte Chrysochromulina polylepis: effect of cell concentration, growth phase and pH. Mar Ecol Prog Ser 216:67–81

    CAS  Google Scholar 

  • Tomas CR (ed), Hasle GR, Syvertsen EE, Steidinger KA, Tangen K, Throndsen J, Heimdal BR (contributors) (1997) Identifying marine phytoplankton. Academic, San Diego, pp 450–451

  • Uchida T, Yamaguchi M, Matsuyama Y, Honjo T (1995) The red-tide dinoflagellate Heterocapsa sp. kills Gyrodinium instriatum by cell contact. Mar Ecol Prog Ser 118:301–303

    Google Scholar 

  • Uchida T, Toda S, Matsuyama Y, Yamaguchi M, Kotani Y, Honjo T (1999) Interactions between the red tide dinoflagellates Heterocapsa circularisquama and Gymnodinium mikimotoi in laboratory culture. J Exp Mar Biol Ecol 241:285–299

    Article  Google Scholar 

  • Vargas-Montero M, Freer E, Jiménez-Montealegre R, Guzmán JC (2006) Occurrence and predominance of fish killer Cochlodinium polykrikoides on the Pacific coast of Costa Rica. Afr J Mar Sci 28:215–217

    Google Scholar 

  • Yamasaki Y, Nagasoe S, Matsubara T, Shikata T, Shimasaki Y, Oshima Y, Honjo T (2007) Allelopathic interactions between the bacillariophyte Skeletonema costatum (Greville) Cleve and the raphidophyte Heterosigma akashiwo (Hada) Hada ex Hara et Chihara. Mar Ecol Prog Ser (in press)

  • Yamatogi T, Sakaguti S, Takagi N, Iwataki M, Matsuoka K (2005) Effects of temperature, salinity and light intensity on the growth of a harmful dinoflagellate Cochlodinium polykrikoides Margalef occurring in coastal waters of West Kyushu, Japan. Bull Plankton Soc Japan 52:4–10 (in Japanese)

    Google Scholar 

  • Yokote M, Honjo T (1985) Morphological and histochemical demonstration of a glycocalyx on the cell surface of Chattonella antiqua, “a naked flagellate”. Experimentia 41:1143–1145

    Article  CAS  Google Scholar 

  • Yokote M, Honjo T, Asakawa M (1985) Histochemical demonstration of a glycocalyx on the cell surface of Heterosigma akashiwo. Mar Biol 88:295–299

    Article  CAS  Google Scholar 

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Acknowledgments

We thank Dr. K. Miyamura of the Marine Fisheries Research Center, Oita Prefecture, for providing seawater samples from Inokushi Bay, Oita Prefecture.

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Authors and Affiliations

  1. Laboratory of Marine Environmental Science, Division of Marine Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan

    Yasuhiro Yamasaki, Tadashi Matsubara, Tomoyuki Shikata, Yohei Shimasaki, Yuji Oshima & Tsuneo Honjo

  2. Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, 2-17-5 Maruishi, Hatsukaichi, Hiroshima, 739-0452, Japan

    Sou Nagasoe

Authors
  1. Yasuhiro Yamasaki
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  2. Sou Nagasoe
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  3. Tadashi Matsubara
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  4. Tomoyuki Shikata
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  5. Yohei Shimasaki
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  6. Yuji Oshima
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  7. Tsuneo Honjo
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Corresponding author

Correspondence to Yasuhiro Yamasaki.

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Communicated by S. Nishida.

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Yamasaki, Y., Nagasoe, S., Matsubara, T. et al. Growth inhibition and formation of morphologically abnormal cells of Akashiwo sanguinea (Hirasaka) G. Hansen et Moestrup by cell contact with Cochlodinium polykrikoides Margalef. Mar Biol 152, 157–163 (2007). https://doi.org/10.1007/s00227-007-0671-7

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  • DOI: https://doi.org/10.1007/s00227-007-0671-7

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