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Antioxidant properties of monomeric, oligomeric, and polymeric fractions in hot water extract from Pinus radiata bark

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Abstract

Hot water extract (HWE) from Pinus radiata bark was fractionated into monomeric polyphenol (MPP), oligomeric proanthocyanidin (OPA), and polymeric proanthocyanidin (PPA) fractions by monitoring the UV-visible spectrum of the eluted fractions. Nine polyphenols were identified in MPP: three phenolic acids and six flavonoids. The major compounds of MPP were taxifolin (456 μg/mg), catechins (240 μg/mg), and protocatechuic acid (46.2 μg/mg). The OPA components ranged from dimers to hexamers. PPA comprised procyanidin (PC, 94%), a small amount of prodelphinidin (PD, 6%), and also the higher trans configuration, rather than the cis configuration, of C2-C3. The average molecular weight (Mw, Mn), polydispersity (Mw/Mn), and DP of PPA were 3,800 (Mw), 1,200 (Mn), 3.2 and 13, respectively. The potential antioxidant activities of HWE and the three fractions were estimated using proton- or electron-donating assays containing 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical cation, superoxide anion (O •−2 ), hydrogen peroxide (H2O2), reducing power and lipid peroxidation. All the bark fractions exhibited potent quenching abilities against both commercially available radicals (DPPH•, ABTS•+) and chemically induced radicals (H2O2, O •−2 ), as well as strong reducing power and inhibitory activity of lipid peroxidation. Particularly, OPA showed a hydrogen peroxide scavenging activity significantly higher (p < 0.05) than that of the other fractions. From 2-thiobarbituric acid reactive substances and ferric thiocyanate assays, PPA was suspected to act by essentially delaying the formation of hydroperoxide by quenching free oxygen radicals that operates in oil-in-water emulsion.

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Acknowledgements

This study was supported by the Technology Development Program for Agricultural and Forestry, Ministry of Agriculture and Forestry, Republic of Korea.

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  1. Department of Advanced Organic Materials Engineering, Chonbuk National University, Jeonbuk, Jeonju, 561-756, South Korea

    Chang Sub Ku

  2. Division of Forest Science, College of Agriculture and Life Sciences, Chonbuk National University, Jeonbuk, Jeonju, 561-756, South Korea

    Sung Phil Mun

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  1. Chang Sub Ku
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Correspondence to Sung Phil Mun.

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Ku, C.S., Mun, S.P. Antioxidant properties of monomeric, oligomeric, and polymeric fractions in hot water extract from Pinus radiata bark. Wood Sci Technol 42, 47–60 (2008). https://doi.org/10.1007/s00226-007-0150-9

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