Abstract.
Cartilage oligomeric matrix protein (COMP) is a macromolecule of yet unknown function with restricted distribution among tissues. In the present study, the ultrastructural localization of COMP in porcine immature joint cartilage and growth plate cartilage was semiquantitatively delineated. Tissues were fixed in a mixture of low concentration glutar- and paraformaldehyde, embedded at low temperature, and subjected to immunocytochemistry using polyclonal antibodies raised against bovine COMP. Protein A-coated colloidal gold was used for detection. The most intense immunolabeling for COMP was noted in the proliferative zones of the growth cartilages. Here the concentration of immunomarker was higher in the territorial compartment than in the pericellular and interterritorial areas. A low concentration of COMP was observed in the resting and hypertrophic zones. The immunolabeling for COMP did not differ between the three matrix compartments of these zones. Supported by previous data obtained by in situ hybridization, the concentration of immunolabeling in the proliferative zone indicates a high rate of COMP synthesis in proliferative chondrocytes. Hence, COMP may be considered as a marker for normal differentiation into proliferative chondrocytes.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 28 November 1995 / Accepted: 15 October 1996
Rights and permissions
About this article
Cite this article
Ekman, S., Reinholt, F., Hultenby, K. et al. Ultrastructural Immunolocalization of Cartilage Oligomeric Matrix Protein (COMP) in Porcine Growth Cartilage . Calcif Tissue Int 60, 547–553 (1997). https://doi.org/10.1007/s002239900278
Published:
Issue Date:
DOI: https://doi.org/10.1007/s002239900278