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Age-Related Changes in Collagen Properties and Mineralization in Cancellous and Cortical Bone in the Porcine Mandibular Condyle

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An Erratum to this article was published on 15 February 2011

Abstract

Collagen is an important constituent of bone, and it has been suggested that changes in collagen and mineral properties of bone are interrelated during growth. The aim of this study was to quantify age-related changes in collagen properties and the degree of mineralization of bone (DMB). The DMB in cancellous and cortical bone samples from the mandibular condyle of 35 female pigs aged 0–100 weeks was determined using micro-computed tomography. Subsequently, the amount of collagen and the number of pentosidine (Pen), hydroxylysylpyridinoline (HP), and lysylpyridinoline (LP) cross-links were quantified by means of high-performance liquid chromatography. The amount of collagen increased with age in cancellous bone but remained unchanged in cortical bone. The number of Pen and LP cross-links decreased in both bone types. In contrast, the number of HP cross-links decreased only in cancellous bone. The sum of the number of HP and LP cross-links decreased with age in cancellous bone only. The DMB increased in cancellous and cortical bone. It was concluded that the largest changes in the number of mature collagen cross-links and the mineralization in porcine cancellous and cortical bone take place before the age of 40 weeks. The low number of mature cross-links after this age suggests that the bone turnover rate continues to be high and thereby prevents the development of mature cross-links.

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Authors and Affiliations

  1. Department of Orthodontics, Academic Center for Dentistry Amsterdam, University of Amsterdam and VU University, Louwesweg 1, 1066 EA, Amsterdam, The Netherlands

    Nop M. B. K. Willems, Thorsten Grünheid & Andrej Zentner

  2. Department of Functional Anatomy, Research Institute MOVE, Academic Center for Dentistry Amsterdam, University of Amsterdam and VU University, Tafelbergweg 51, 1105 BD, Amsterdam, The Netherlands

    Nop M. B. K. Willems, Geerling E. J. Langenbach, Lars Mulder & Theo M. G. J. van Eijden

  3. Department of Oral Cell Biology, Research Institute MOVE, Academic Center for Dentistry Amsterdam, University of Amsterdam and VU University, Van der Boechorststraat 7, 1081 BT, Amsterdam, The Netherlands

    Vincent Everts & Ruud A. Bank

  4. Department of Biomedical Engineering, Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, The Netherlands

    Lars Mulder

  5. TNO Quality of Life, Zernikedreef 9, 2333 CK, Leiden, The Netherlands

    Ruud A. Bank

Authors
  1. Nop M. B. K. Willems
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  2. Geerling E. J. Langenbach
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  3. Vincent Everts
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  4. Lars Mulder
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  5. Thorsten Grünheid
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  6. Ruud A. Bank
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  7. Andrej Zentner
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  8. Theo M. G. J. van Eijden
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Correspondence to Nop M. B. K. Willems.

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The authors have stated that they have no conflict of interest.

An erratum to this article can be found at http://dx.doi.org/10.1007/s00223-011-9469-1

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223_2010_9339_MOESM1_ESM.eps

Supplementary material 1 Frontal view of a mandibular condyle (transparent). Cancellous and cortical bone samples were obtained near the center and near the surface of the condyle, respectively. The distribution of the mineral in the samples is shown. Scale: the degree of mineralization of bone (DMB) increases from green to red. Please note this is a left condyle.(EPS 639 kb)

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Willems, N.M.B.K., Langenbach, G.E.J., Everts, V. et al. Age-Related Changes in Collagen Properties and Mineralization in Cancellous and Cortical Bone in the Porcine Mandibular Condyle. Calcif Tissue Int 86, 307–312 (2010). https://doi.org/10.1007/s00223-010-9339-2

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  • DOI: https://doi.org/10.1007/s00223-010-9339-2

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