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Comparison of Multipotent Differentiation Potentials of Murine Primary Bone Marrow Stromal Cells and Mesenchymal Stem Cell Line C3H10T1/2

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Abstract

Murine C3H10T1/2 cells have many features of mesenchymal stem cells (MSCs). Whether or not the multipotent differentiation capability of C3H10T1/2 cells is comparable to that of primary bone marrow–derived MSCs (BM-MSCs) was investigated in this study. For in vitro osteogenic differentiation, both BM-MSCs and C3H10T1/2 cells differentiated to osteoblastic cell lineage and showed positive staining for alkaline phosphatase (ALP) and increased mRNA expression of Runx2, Col1αI, and osteocalcin. C3H10T1/2 cells and BM-MSCs induced similar amounts of bone formation in the biomaterials. Under chondrogenic induction in the presence of TGF-β1, cell pellets of both BM-MSCs and C3H10T1/2 cells formed cartilage-like tissues with cartilage matrix components including proteoglycan, type II collagen, and aggrecan. However, C3H10T1/2 cells presented lower adipogenic differentiation potential, with only about 10% C3H10T1/2 cells (but about 70% of BM-MSCs) being committed to adipogenesis. In this study we confirmed that C3H10T1/2 cells coimplanted with osteoconductive scaffolds can form bone spontaneously in vivo and that C3H10T1/2 cells have a basal level of osteocalcin expression, suggesting that they may be a good alternative source of primary BM-MSCs for investigating osteogenic and chondrogenic differentiation in bone or cartilage tissue engineering studies. Caution is needed when using C3H10T1/2 cells for adipogenic studies as they appear to have lower adipogenic potential than BM-MSCs.

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Acknowledgments

L. Z. was supported by an Overseas Research Student Award from Universities UK and a PhD studentship from Queen’s University Belfast, UK (2004–2007).

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Authors and Affiliations

  1. Centre for Cancer Research and Cell Biology, School of Biomedical Sciences, Queen’s University Belfast, 97 Lisburn Road, Belfast, BT7 1BL, UK

    Li Zhao & Gang Li

  2. Department of Orthopedics and Traumatology, CUHK-WHO Collaborating Centre for Sports Medicine and Health Promotion, Chinese University Hong Kong, Clinical Sciences Building, Prince of Wales Hospital, Shatin, Hong Kong, People’s Republic of China

    Gang Li & Kai-Ming Chan

  3. Department of Orthopedic Surgery, General Hospital of Chinese People’s Liberation Army, Beijing, 100853, People’s Republic of China

    Yan Wang & Pei-Fu Tang

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  1. Li Zhao
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  2. Gang Li
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Correspondence to Pei-Fu Tang.

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Zhao, L., Li, G., Chan, KM. et al. Comparison of Multipotent Differentiation Potentials of Murine Primary Bone Marrow Stromal Cells and Mesenchymal Stem Cell Line C3H10T1/2. Calcif Tissue Int 84, 56–64 (2009). https://doi.org/10.1007/s00223-008-9189-3

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  • DOI: https://doi.org/10.1007/s00223-008-9189-3

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