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B Cells Play an Important Role in Lipopolysaccharide-Induced Bone Resorption

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Abstract

The host immune system, especially activated T cells, plays a crucial role in inflammatory bone resorption and osteoclastogenesis. Previously, we showed that T cells are involved in inflammatory bone resorption in vivo. However, little is known about whether B cells are involved in inflammatory bone resorption and how B cells take part in osteoclastogenesis. Therefore, the aim of this study was to examine whether B c ells truly influence inflammatory bone resorption in vivo. Alveolar bone resorption in normal mice, in SCID mice that lack both B and T cells, and in B cell-reconstituted SCID mice was compared histopathologically after repeated injections of lipopolysaccharide (LPS) into the gingiva. Furthermore, we examined whether the B cells that are stimulated by LPS are involved in osteoclastogenesis in vitro. As a result, the B cell-reconstituted SCID mice showed stronger inflammatory bone resorption than the SCID mice. Also, in vitro, LPS-stimulated B cells enhanced osteoclastogenesis and anti-tumor necrosis factor (TNF)-α antibody completely blocked osteoclastogenesis induced by LPS-stimulated B cells. These results suggest that B cells promote inflammatory bone resorption through TNF-α.

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Authors and Affiliations

  1. Division of Periodontology, Department of Developmental and Reconstructive Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8588, Japan

    Y. Kozuka, Y. Ozaki, T. Ukai, T. Kaneko & Y. Hara

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  1. Y. Kozuka
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  2. Y. Ozaki
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  3. T. Ukai
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  4. T. Kaneko
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  5. Y. Hara
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Correspondence to Y. Hara.

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Kozuka, Y., Ozaki, Y., Ukai, T. et al. B Cells Play an Important Role in Lipopolysaccharide-Induced Bone Resorption. Calcif Tissue Int 78, 125–132 (2006). https://doi.org/10.1007/s00223-005-0149-x

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  • DOI: https://doi.org/10.1007/s00223-005-0149-x

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