Abstract
Chondrocytes in the growth plate undergo a characteristic sequel of differentiation processes, finally leading in their death by apoptosis. The mechanisms that mediate these remarkable biological phenomenon are still widely unknown. For this study, growth plates from five resected polydactylic infantile fingers were taken and immunostained for APO-1 and Caspase-8, two apoptosis-mediating proteins. The expression of both proteins was examined in the resting zone, proliferating zone, and hypertrophic zone, representing the maturation stages of the growth plate. Positive cells for APO-1 and Caspase-8 were found in all zones of the physis with a significant increase from the resting to the hypertrophic zone (from 17.4% to 33.067% for APO-1 and from 13.17% to 28.22% for Caspase-8). Our findings suggest that both APO-1 and Caspase-8 are involved in chondrocyte development in the growth plate, predominantly near to the closure area.
Similar content being viewed by others
References
CE Farnum NJ Wilsmann (1989) ArticleTitleCellular turnover at the chondro-osseous junction of growth plate cartilage: analysis by serial sections at the light microscopal level. J Orthop Res 7 654–666 Occurrence Handle1:STN:280:BiaA2c7pvFY%3D Occurrence Handle2760737
D Lewinson M Silbermann (1992) ArticleTitleChondroclasts and endothelial cell collaborate in the process of cartilage resorption. Anat Rec 233 504–514 Occurrence Handle1:STN:280:By2A38fitFI%3D Occurrence Handle1626710
D Chrysis O Nilsson EM Ritzen L Savendahl (2002) ArticleTitleApoptosis is developmentally regulated in rat growth plate. Endocrine 18 271–278 Occurrence Handle10.1385/ENDO:18:3:271 Occurrence Handle1:CAS:528:DC%2BD38Xos1Crsr8%3D Occurrence Handle12450319
WW Jr Robertson (1990) ArticleTitleNewest knowledge of the growth plate. Clin Orthop 253 270–278 Occurrence Handle2180603
J Ogasawara R Watanabe-Fukunaga M Adachi A Matsuzawa T Kasugai Y Kitamura N Itoh T Suda S Nagata (1993) ArticleTitleLethal effect of the anti-Fas antibody mice. Nature 364 806–809 Occurrence Handle1:CAS:528:DyaK3sXmt1elurg%3D Occurrence Handle7689176
K Trieb T Lechleitner S Lang R Windhager R Kotz S Dirnhofer (1998) ArticleTitleHeat shock protein 72 expression in osteosarcomas correlates with good response to neoadjuvant chemotherapy. Hum Pathol 29 IssueID10 1050–1055 Occurrence Handle1:CAS:528:DyaK1cXntFGgsbo%3D Occurrence Handle9781640
Y Gavrieli Y Sherman SA Ben-Sasson (1992) ArticleTitleIdentification of programmed cell death in situ via specific labelling of nuclear DNA fragmentation. J Cell Biol 119 493–501 Occurrence Handle1:CAS:528:DyaK38XmtVyhsbs%3D Occurrence Handle1400587
T Ueda K Aozasa M Tsujimoto M Ohsawa A Uchida Y Aoki K Ono K Matsumoto (1989) ArticleTitlePrognostic significance of Ki-67 reactivity in soft tissue sarcomas. Cancer 32 219–229
JF Kerr AH Wyllie AR Currie (1972) ArticleTitleApoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer 26 239–257 Occurrence Handle4561027
EB Hunziker (1994) ArticleTitleMechanisms of longitudinal bone growth and its regulation by growth plate chondrocytes. Microsc Res Tech 28 505–519 Occurrence Handle1:STN:280:ByqD3snmtlQ%3D Occurrence Handle7949396
O Nilsson V Abad D Chrysis EM Ritzen L Savendahl J Baron (2002) ArticleTitleEstrogen receptor-alpha and -beta are expressed throughout postnatal development in the rat and rabbit growth plate. J Endocrinol 173 407–414 Occurrence Handle1:CAS:528:DC%2BD38Xlt1ehtLk%3D Occurrence Handle12065230
OG Isaksson A Lindahl A Nilsson J Isgaard (1987) ArticleTitleMechanism of stimulatory effect of growth hormone on longitudinal bone growth. Endocrine Rev 8 426–438 Occurrence Handle1:CAS:528:DyaL1cXkvFeluw%3D%3D
EB Hunziker J Wagner J Zapf (1994) ArticleTitleDifferential effects of IGF-1 and hGH on the various developmental stages of growth plate chondrocytes in vivo. J Clin Invest 93 1078–1086 Occurrence Handle1:CAS:528:DyaK2cXitV2guro%3D Occurrence Handle8132746
OG Isaksson A Nilsson J Isgaard A Lindahl (1990) ArticleTitleCartilage as a target tissue for growth hormone and insulin-like growth factor 1. Acta Paediatr Scand 367 IssueIDsuppl 137–141 Occurrence Handle1:STN:280:By6D383gtVA%3D
A Vortkamp K Lee B Lanske GV Segre HM Kronenberg CJ Tabin (1996) ArticleTitleRegulation of rate of cartilage differentation by Indian Hedgehog and PTH-related protein. Science 273 613–622 Occurrence Handle1:CAS:528:DyaK28Xks1yntL4%3D Occurrence Handle8662546
T Aizawa S Kokubun T Kawamata Y Tanaka HI Roach (1999) ArticleTitleC-myc protein in the rabbit growth plate. J Bone Joint Surg 81-B 921–925 Occurrence Handle10.1302/0301-620X.81B5.9492
FJ Blanco RL Ochs H Schwarz M Lotz (1995) ArticleTitleChondrocyte apoptosis induced by nitric oxide. Am J Pathol 146 75–85 Occurrence Handle1:CAS:528:DyaK2MXjs1yjurg%3D Occurrence Handle7856740
CC Teixeira K Mansfield C Hertkorn H Ischiropoulos IM Shapiro (2001) ArticleTitlePhosphate-induced chondrocyte apoptosis is linked to nitric oxide generation. Am J Physiol Cell Physiol 281 833–839
S Hashimoto M Setareh RL Ochs M Lotz (1997) ArticleTitleFas/Fas ligand expression and induction of apoptosis in chondrocytes. Arthritis Rheum 40 1749–1755
T Aizawa HI Roach S Kokubun Y Tanaka (1998) ArticleTitleChanges in the expression of Fas, osteonectin and osteocalcin with age in the rabbit growth plate. J Bone Joint Surg 80B 880–887 Occurrence Handle10.1302/0301-620X.80B5.8430
K Kuida TF Haydar C Kuan Y Gu C Taya H Karasuyama MS Su P Rakic RA Flavell (1998) ArticleTitleReduced apoptosis and cytochrome c-mediated caspase activation in mice lacking caspase 9. Cell 94 325–337 Occurrence Handle1:STN:280:DyaK1cznt1Cnsg%3D%3D Occurrence Handle9708735
R Hakem A Hakem GS Duncan et al. (1998) ArticleTitleDifferential requirement for caspase 9 in apoptotic pathways in vivo. Cell 94 339–352 Occurrence Handle1:STN:280:DyaK1cznt1Cnsw%3D%3D Occurrence Handle9708736
F Cecconi G Alvarez-Bolado BI Meyer KA Roth P Gruss (1998) ArticleTitleApaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94 727–737 Occurrence Handle1:CAS:528:DyaK1cXmtlCjs7s%3D Occurrence Handle9753320
Acknowledgements
This study was supported by a grant from the “Wissenschaftlichen Fonds des Bürgermeisters der Bundeshauptstadt Wien,” project number 1978.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cetin, E., Girsch, W., Brand, G. et al. Distinct Expression of APO-1/Fas and Caspase-8 in the Human Growth Plate . Calcif Tissue Int 74, 181–186 (2004). https://doi.org/10.1007/s00223-002-2161-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00223-002-2161-8