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Evaluating in vitro dipeptidyl peptidase IV inhibition by peptides from common carp (Cyprinus carpio) roe in cell culture models

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Abstract

Dipeptidyl peptidase IV (DPP-IV) inhibitors have become a new choice to treat diabetes. Here, we studied the DPP-IV inhibitory activity of protein hydrolysates from common carp (Cyprinus carpio) roe. The molecular weight distribution and simulated gastrointestinal digestive stability of hydrolysates produced by four enzymes were analyzed. Papain-generated hydrolysate showed the highest DPP-IV inhibition and effective gastrointestinal stability. New effective peptide sequences from papain hydrolysates were identified using liquid chromatography-tandem mass spectrometry. The bioactivity of IPNVAVD, which was identified from papain hydrolysate (IC50 value of 777.35 ± 5.50 μM), was investigated by Caco-2 and HepG2 cell models. We found that the DPP-IV inhibition by papain hydrolysate was not attenuated after simulated gastrointestinal digestion. In addition, IPNVAVD significantly inhibited the DPP-IV secreted by Caco-2 cells with no cytotoxicity. It also promoted glucose uptake in insulin-resistant HepG2 cells. Transport experiments showed that IPNVAVD could be absorbed intactly by the Caco-2 cell monolayer. Protein hydrolysates from common carp roe inhibited DPP-IV effectively, and they may have potential as functional ingredients in food for diabetic patients.

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Acknowledgements

This study was supported by the National Key R&D Program of China (award number 2017YFD0400201) and Earmarked Fund for China Agriculture Research System (CARS-45).

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Authors and Affiliations

  1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 10083, China

    Changyu Zhang, Song Gao, Yuqi Zhang, Hui Hong & Yongkang Luo

  2. National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang, Jiangxi, 330022, China

    Yongkang Luo

  3. School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, 10037, China

    Chi Zhang

  4. Anhui Guotai Biotechnology Co., Ltd., Xuancheng, 242000, Anhui, China

    Huaigao Liu

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  1. Changyu Zhang
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Correspondence to Yongkang Luo.

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The entire experimental procedure was in accordance with the Guidance on Treating Experimental Animals developed by China’s Ministry of Science & Technology in 2006 and Regulations issued by China State Council in 1988.

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Zhang, C., Gao, S., Zhang, C. et al. Evaluating in vitro dipeptidyl peptidase IV inhibition by peptides from common carp (Cyprinus carpio) roe in cell culture models. Eur Food Res Technol 246, 179–191 (2020). https://doi.org/10.1007/s00217-019-03399-6

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