Abstract
Quinoa (Chenopodium quinoa) is a dicotyledonous plant producing perispermic seeds that is increasingly popular in the Western world due to its nutritional value. Germination can be used in food processing to alter the sensory, nutritional and functional properties of grains, and the increased enzyme activities may have beneficial influences in food applications. Knowledge about the germination of perispermic seeds is scarce. In this study, the development of amylolytic activities and subsequent changes in sugar profiles and starch content were followed in quinoa over a period of 72 h. The seeds germinated rapidly with radicle protrusion occurring 8 h after imbibition, when the seeds had reached a moisture content of 44 %. A low level of α-amylase activity (determined at pH 5.2 using blocked ρ-nitrophenyl-maltoheptaoside as substrate) was present in the embryo of non-germinating seeds, but emerged in the perisperm only after 24 h, followed by a sharp increase in activity in both tissues. The accumulation of glucose and fructose (determined with high pressure liquid chromatography) and the decrease in starch content (determined with an enzymatic assay) were observed after 24 and 36 h, respectively, indicating the onset of starch reserve mobilization. Overall, the levels of amylolytic activities remained very low compared with traditional malting cereals, suggesting the unsuitability of quinoa as a source of amylases in food applications. Scanning electron microscopy, used to visualize changes in starch granule morphology, showed mainly exocorrosion, suggesting a homogenous structure of the outer layers of quinoa starch.
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Abbreviations
- BpNPG7:
-
Blocked p-Nitrophenyl-α-d-maltoheptaoside
- CU:
-
Ceralpha unit
- Dwb:
-
Dry weight based
- Hai:
-
Hours after imbibition
- HPLC:
-
High pressure liquid chromatography
- SEM:
-
Scanning electron microscopy
References
James A, Lilian E (2009) Quinoa (Chenopodium Quinoa Willd.): composition, chemistry, nutritional, and functional properties. Adv Food Nutr Res 58:1–31
Jacobsen SE, Mujica A, Ortiz R (2003) The global potential for quinoa and other andean crops. Food Rev Int 19:139–148
Valencia-Chamorro S (2003) Quinoa. In: Caballero B (ed) Encyclopedia of food science and nutrition. Academic Press, Amsterdam
Prego I, Maldonado S, Otegui M (1998) Seed structure and localization of reserves in Chenopodium quinoa. Ann Bot 82(4):481–488
Lawrence DM, Halmer P, Bowles DJ (1990) Mobilisation of storage reserves during germination and early seedling growth of sugar beet. Physiol Plant 78:421–429
Elgeti D, Nordlohne SD, Föste M, Besla M, Linden MH, Heinz V, Jekle M, Becker T (2014) Volume and texture improvement of gluten-free bread using quinoa white flour. J Cereal Sci 59(1):41–47
Bergamo P, Maurano F, Mazzarella G, Iaquinto G, Vocca I, Rivelli AR, De Falco E, Gianfrani C, Rossi M (2011) Immunological evaluation of the alcohol-soluble protein fraction from gluten-free grains in relation to celiac disease. Mol Nutr Food Res 55:1266–1270
Lee AR, Ng DL, Dave E, Ciaccio EJ, Green PHR (2009) The effect of substituting alternative grains in the diet on the nutritional profile of the gluten-free diet. J Hum Nutr Diet 22:359–363
Ranhotra GS, Gelroth JA, Glaser BK, Lorenz KJ, Johnson DL (1993) Composition and protein nutritional quality of quinoa. Cereal Chem 70:303–305
Hager A-S, Wolter A, Jacob F, Zannini E, Arendt EK (2012) Nutritional properties and ultra-structure of commercial gluten free flours from different botanical sources compared to wheat flours. J Cereal Sci 56:239–247
Bewley D, Black M (1985) Seeds: germination, structure and composition. In: Bewley D, Black M (eds) Seeds—physiology of development and germination. Plenum Press, New York, pp 1–25
Mikola M (2001) Electrophoretic studies on the endoproteinases of oat grain, Ph.D. Thesis, University of Helsinki, Finland
Mäkinen OE, Arendt EK (2012) Oat malt as a baking ingredient—a comparative study of the impact of oat, barley and wheat malts on bread and dough properties. J Cereal Sci 56:747–753
Mäkinen OE, Zannini E, Arendt EK (2013) Germination of oat and quinoa and evaluation of the malts as gluten free baking ingredients. Plant Foods Hum Nutr 68:90–95
Larsson M, Rossander-Hulthén L, Sandström B, Sandberg AS (1996) Improved zinc and iron absorption from breakfast meals containing malted oats with reduced phytate content. Br J Nutr 76:677–688
Valencia S, Svanberg U, Sandberg AS (1999) Processing of quinoa (Chenopodium Quinoa, Willd): effects on in vitro iron availability and phytate hydrolysis. Int J Food Sci Nutr 50:203–211
Alvarez-Jubete L, Wijngaard H, Arendt AK, Gallagher E (2010) Polyphenol composition and in vitro antioxidant activity of amaranth, quinoa buckwheat and wheat as affected by sprouting and baking. Food Chem 119:770–778
Fincher GB (1989) Molecular and cellular biology associated with endosperm mobilization in germinating cereal grains. Annu Rev Plant Physiol Plant Mol Biol 40:305–346
Rosa M, Hilal M, González JA, Prado FE (2004) Changes in soluble carbohydrates and related enzymes induced by low temperature during early developmental stages of quinoa (Chenopodium quinoa) seedlings. J Plant Physiol 161:683–689
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Belay G, Furuta Y (2001) Zymogram patterns of α-amylase isozymes in Ethiopian tetraploid wheat landraces: insight into their evolutionary history and evidence for gene flow. Genet Resour Crop Evol 48:507–512
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Natur 227:680–685
Westermeier R (2006) Sensitive, quantitative, and fast modifications for coomassie blue staining of polyacrylamide gels. Proteomics 6(S2):61–64
Indyk HE, Edwards MJ, Woollardb DC (1996) High performance liquid chromatographic analysis of lactose-hydrolysed milk. Food Chem 57:575–580
Bassinello PZ, Cordenunsi BR, Lajolo FM (2002) Amylolytic activity in fruits: comparison of different substrates and methods using banana as model. J Agric Food Chem 50:5781–5786
Agu RC, Palmer GH (1997) α-Glucosidase activity of sorghum and barley malts. J Inst Brew 103(1):25–29
Atwell W, Hyldon R, Godfrey P, Galle E, Sperber W, Pedersen D, Evans W, Rabe G (1988) Germinated quinoa flour to reduce the viscosity of starchy foods. Cereal Chem 65:508–509
Zarnkow M, Geyer T, Lindemann B, Burberg F, Back W, Arendt EK, Kreisz S (2007) The use of response surface methodology to ptomise malting conditions of quinoa (Chenopodium quinoa L.) as a raw material for gluten-free foods and beverages. Brew Sci 9–10:118–126
Catusse J, Job C, Job D (2012) Proteomics reveals a potential role of the perisperm in starch remobilization during sugarbeet seed germination. In: Rakwal R (ed) Seed development: AgrawalmGK, OMICS technologies toward improvement of seed quality and crop yield. Springer, Dordrect
Rosa M, Hilal M, González JA, Prado FE (2009) Low-temperature effect on enzyme activities involved in sucrose-starch partitioning in salt-stressed and salt-acclimated cotyledons of quinoa (Chenopodium quinoa Willd.) seedlings. Plant Physiol Biochem 47:300–307
Wijngaard HH, Renzetti S, Arendt EK (2007) Microstructure of buckwheat and barley during malting observed by confocal scanning laser microscopy and scanning electron microscopy. J Inst Brew 113(1):34–41
Zarnkow M, Mauch A, Back W, Arendt EK, Kreisz S (2007) Proso millet (Panicum miliaceum L.): an evaluation of the microstructural changes in the endosperm during the malting process by using scanning-electron and confocal laser microscopy. J Inst Brewing 113(4):355–364
Tang H, Watanabe K, Mitsunaga T (2002) Characterization of storage starches from quinoa, barley and adzuki seeds. Carbohydr Polym 49:13–22
Yaldagard M, Mortazavi SA, Tabatabaie F (2008) Influence of ultrasonic stimulation on the germination of barley seed and its alpha-amylase activity. Afr J Biotechnol 7(14):2465–2471
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This research was funded by the Irish Research Council and the Food Institutional Research Measure administered by the Department of Agriculture, Fisheries and Food (Ireland).
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Hager, AS., Mäkinen, O.E. & Arendt, E.K. Amylolytic activities and starch reserve mobilization during the germination of quinoa. Eur Food Res Technol 239, 621–627 (2014). https://doi.org/10.1007/s00217-014-2258-0
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DOI: https://doi.org/10.1007/s00217-014-2258-0