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Purification, characterization, and production of β-mannanase from Bacillus subtilis TJ-102 and its application in gluco-mannooligosaccharides preparation

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Abstract

A novel β-mannanase-producing strain, Bacillus subtilis TJ-102, was identified and characterized. Response surface method was applied to improving and enhancing the enzyme production. The optimized media components were obtained: 45.25 g/L konjac, 9.29 g/L Na2HPO4·12H2O, 2.60 g/L CaCO3, 1.0 g/L (NH4)2SO4, 0.3 g/L KH2PO4, 1.0 g/L NaCl, 1.0 g/L MgCl2·6H2O, and 0.01 g/L FeSO4. Under these conditions, the β-mannanase activity could achieve 205.3 U/mL in a 7-L fermentor. Then, β-mannanase was 7.39-fold purified by salting out, ultrafiltration, anion-exchange, and size-exclusion preparative chromatography with a recovery of 21.41 % and a specificity of 125.36 U/mg proteins. β-Mannanase was stable below 65 °C and pH 5.0–8.0, which exhibited excellently enzymatic efficiency in the preparation of gluco-mannooligosaccharides (GMOS) by hydrolyzing konjac flour. The GMOS yield of 57.76 % has been achieved with 8.71 % of mannose and 14.49 % of glucose, demonstrating the potential use of β-mannanase in food industry.

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Acknowledgments

This work was supported by the Program for New Century Excellent Talents in Chinese University (NCET-08-0386), the 863 Program of China (2008AA10Z318), the Natural Science Foundation of China (20976125; 31071509) and Tianjin (10JCYBJC05100), and the Program of Introducing Talents of Discipline to Universities of China (No. B06006).

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None.

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This article does not contain any studies with human or animal subjects.

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Authors and Affiliations

  1. Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Mengfan Wang, Shengping You, Shuaishuai Zhang, Wei Qi, Zhaohui Liu, Weina Wu, Rongxin Su & Zhimin He

  2. State Key Laboratory of Chemical Engineering, Tianjin University, Tianjin, 300072, People’s Republic of China

    Wei Qi, Rongxin Su & Zhimin He

  3. Tianjin Key Laboratory of Membrane Science and Desalination Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Mengfan Wang, Wei Qi & Rongxin Su

  4. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

    Mengfan Wang

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  1. Mengfan Wang
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Correspondence to Wei Qi.

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Mengfan Wang and Shengping You have contributed equally to this work.

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Wang, M., You, S., Zhang, S. et al. Purification, characterization, and production of β-mannanase from Bacillus subtilis TJ-102 and its application in gluco-mannooligosaccharides preparation. Eur Food Res Technol 237, 399–408 (2013). https://doi.org/10.1007/s00217-013-2002-1

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