Skip to main content
SpringerLink
Log in

Diversity and frequencies of methoxypyrazines in hemolymph of Harmonia axyridis and Coccinella septempunctata and their influence on the taste of wine

  • Original Paper
  • Published:
European Food Research and Technology Aims and scope Submit manuscript

Abstract

The study focuses on the odor-active intensity and occurrence of three methoxypyrazines in ladybird beetles, Harmonia axyridis, invasive in Europe and the USA, and Coccinella septempunctata, native in Europe, but invasive in the USA. It is known from previous studies that H. axyridis has negatively influenced the taste of wine in the USA because of releasing their hemolymph in must during harvesting. In relation to C. septempunctata, H. axyridis had more nitrogen containing compounds in its hemolymph and 2-isopropyl-3-methoxypyrazine (IPMP) and mainly 2-sec-buthyl-3-methoxypyrazine (SBMP) in higher amounts. The compound IPMP was detected as the main odor-active compound in both ladybird beetle species. Wines infested with hemolymph of C. septempunctata were more readily detected as altered wine with a ‘ladybird taint’ than wines infested with H. axyridis. 2-isobuthyl-3-methoxypyrazine (IBMP) could be identified as second intensive odor-active compound of C. septempunctata, which occurred in a much lower frequency in H. axyridis. SBMP, the second highest measured N-compound of H. axyridis, had a lower olfactory active threshold in gas chromatography–olfactometry test. C. septempunctata contained less SBMP in its hemolymph than H. axyridis. This study objected that besides H. axyridis also C. septempunctata can influence the taste of wine in a detectable way.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Galvan T, Koch R, Hutchison WD (2008) Impact of fruit feeding on overwintering survival of the multicolored Asian lady beetle, and the ability of this insect and paper wasps to injure wine grape berries. Entomol Exp Appl 128:429–436

    Article  Google Scholar 

  2. Riesen R (2008) Der asiatische Marienkäfer im Weinbau. Schweiz Z Obst-Weinbau 22:10–12

    Google Scholar 

  3. Cai LS, Koziel JA, O’Neal ME (2007) Determination of characteristic odorants from Harmonia axyridis beetles using in vivo solid-phase microextraction and multidimensional gas chromatography-mass spectrometry-olfactometry. J Chromatogr A 1147:66–78

    Article  CAS  Google Scholar 

  4. Galvan TL, Burkness EC, Vickers Z, Stenberg P, Mansfield AK, Hutchison WD (2007) Sensory-based action threshold for multicolored Asian lady beetle-related taint in winegrapes. Am J Enol Viticult 58:518–522

    Google Scholar 

  5. Pickering G, Lin J, Riesen R, Reynolds A, Brindle I, Soleas G (2004) Influence of Harmonia axyridis on the sensory properties of white and red wine. Am J Enol Viticult 55:153–159

    Google Scholar 

  6. Pickering G, Ker K, Soleas G (2007) Determination of the critical stages of processing and tolerance limits for Harmonia axyridis for ‘ladybug taint’ in wine. Vitis 46:85–90

    Google Scholar 

  7. Ross CF, Rosales MU, Fernandez-Plotka VC (2010) Aroma profile of Niagara grape juice contaminated with multicoloured Asian lady beetle taint using gas chromatography/mass spectrometry/olfactometry. Intl J Food Sci Tech 45:789–793

    Article  CAS  Google Scholar 

  8. Mohr HD (2005) Farbatlas Krankheiten, Schädlinge und Nützlinge an der Weinrebe. Eugen Ulmer KG

  9. Cudjoe E, Wiederkehr TB, Brindle ID (2005) Headspace gas chromatography-mass spectrometry: a fast approach to the identification and determination of 2-alkyl-3-methoxypyrazine pheromones in ladybugs. Analyst 130:152–155

    Article  CAS  Google Scholar 

  10. Al Abassi S, Birkett MA, Pettersson J, Pickett JA, Woodcock CM (1998) Ladybird beetle odour identified and found to be responsible for attraction between adults. Cell Mol Life Sci 54:876–879

    Article  CAS  Google Scholar 

  11. Ullrich W, Grosch W (1987) Identification of the most intense volatile flavour compounds formed during autoxidation of linoleic acid. Z Lebensm Unters Forsch 184:277–282

    Article  CAS  Google Scholar 

  12. Linder C, Lorenzini F, Kehrli P (2009) Potential impact of processed Harmonia axyridis on the taste of ‘Chasselas’ and ‘Pinot noir’ wines. Vitis 48:101–102

    CAS  Google Scholar 

  13. Kögel S, Gross J, Hoffmann C (2012) Sensory detection thresholds of ‘ladybird taint’ in ‘Riesling’ and ‘Pinot noir’ under different fermentation and processing conditions. Vitis (in press)

  14. Galvan TL, Kells S, Hutchison WD (2008) Determination of 3-alkyl-2-methoxypyrazines in lady beetle-infested wine by solid-phase microextraction headspace sampling. J Agric Food Chem 56:1065–1071

    Article  CAS  Google Scholar 

  15. Sala C, Mestres M, Marti MP, Busto O, Guasch J (2002) Headspace solid-phase microextraction analysis of 3-alkyl-2-methoxypyrazines in wines. J Chromatogr A 953:1–6

    Article  CAS  Google Scholar 

  16. Savoiskaya GI (1970) Introduction and acclimatisation of some coccinellids in the Alma-Ata reserve. Trudy Alma Atinskogo Gosudarstvennogo Zapovednika 9:138–162 citated from Koch RL 2003

  17. Koch R (2003) The multicolored Asian lady beetle, Harmonia axyridis: A review of its biology, uses in biological control, and non-target impacts. J Insect Sci 3:1–16

    Article  Google Scholar 

  18. Gross J, Eben A, Mueller I, Wensing A (2010) A well protected intruder: the effective antimicrobial defense of the invasive ladybird Harmonia axyridis. J Chem Ecol 36:1180–1188

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from the ‘Forschungsring deutscher Weinbau’ (FDW). The authors thank the staff of JKI Grape-breeding Institut for support in vinification and wine tasting and the staff of JKI EPS Quedlinburg for sniffing. We thank Svenja Hoferer and Sabine Wetzel (JKI Dossenheim) for rearing the beetles. We are grateful to M. Paulus and C. Emmerling from the Trier University, Faculty VI, (Germany) for cooperation.

Author information

Authors and Affiliations

  1. Julius Kühn-Institut, Federal Research Center for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Geilweilerhof, 76833, Siebeldingen, Germany

    Susanne Kögel & Christoph Hoffmann

  2. Julius Kühn-Institut, Federal Research Center for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Schwabenheimer Str. 101, 69221, Dossenheim, Germany

    Jürgen Gross

  3. Julius Kühn-Institut, Federal Research Center for Cultivated Plants, Institute for Ecological Chemistry, Plant Analysis and Stored Product Protection, Erwin-Baur-Str. 27, 06484, Quedlinburg, Germany

    Detlef Ulrich

Authors
  1. Susanne Kögel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jürgen Gross
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Christoph Hoffmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Detlef Ulrich
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Susanne Kögel.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kögel, S., Gross, J., Hoffmann, C. et al. Diversity and frequencies of methoxypyrazines in hemolymph of Harmonia axyridis and Coccinella septempunctata and their influence on the taste of wine. Eur Food Res Technol 234, 399–404 (2012). https://doi.org/10.1007/s00217-011-1646-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00217-011-1646-y

Keywords

Search

Navigation