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Isolation and identification of a fungal strain QY229 producing milk-clotting enzyme

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Abstract

A strain QY229, which produces a milk-clotting enzyme, was screened from red kojic rice, a Chinese traditional natural food colorant and preservative for meat and fish for centuries. This strain was identified as Quambalaria cyanescens (QY229) based on morphology and internal transcriptional spacer sequence. Inhibition assay by pepstatin A at 10 μM proved it to be an aspartate proteinase. This is the first report about Quambalaria cyanescens producing milk-clotting enzymes.

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Abbreviations

PMSF:

Phenylmethylsulphonyl fluoride

E-64:

Trans-epoxysuccinyl-l-leucylamido (4-guanidino) butane

EDTA:

Ethylenediaminetetraacetic acid

MCA:

Milk-clotting activity

PA:

Proteolytic activity

IDF:

International dairy federation

IMCU:

International milk clotting units

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Acknowledgments

This work was supported by the Shandong Province Science and Technology Tackle Project (Grant no: 2007GG20010004).

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Authors and Affiliations

  1. College of Food Science and Technology, Nanjing Agricultural University, No. 1 Tongwei Road, Nanjing, Jiangsu, China

    Zhiguo Zhang, Zhengying Yao, Junfeng Zhao, Fengxia Lu & Zhaoxin Lu

  2. The Key Laboratory of Microbial Engineering of Shandong Province, Shandong Polytechnic University, University Road, Jinan, Shandong, China

    Zhiguo Zhang, Chenzhong Wang, Gongming Yu & Wenjun Lan

Authors
  1. Zhiguo Zhang
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  2. Chenzhong Wang
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  3. Zhengying Yao
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  4. Junfeng Zhao
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  5. Fengxia Lu
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  6. Gongming Yu
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  7. Wenjun Lan
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  8. Zhaoxin Lu
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Correspondence to Zhaoxin Lu.

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Zhang, Z., Wang, C., Yao, Z. et al. Isolation and identification of a fungal strain QY229 producing milk-clotting enzyme. Eur Food Res Technol 232, 861–866 (2011). https://doi.org/10.1007/s00217-011-1454-4

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  • DOI: https://doi.org/10.1007/s00217-011-1454-4

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