Abstract
A rapid immunochromatographic assay (ICA) was developed for the detection of semicarbazide (SEM), a nitrofurazone metabolite in meat specimens. Colloidal gold–labeled monoclonal antibody specific to a SEM derivative, 4[(4-carboxyphenyl)methylene]-hydrazinecarboxamide (CPSEM), was used as a probe for the presence of SEM. The assay is based upon the competitive reactivity theory that SEM derivatized with 4-carboxybenzaldehyde (4-CBA) competes with CPSEM-conjugated bovine serum albumin for binding the colloidal gold–labeled monoclonal antibody, the result of which could be read directly by naked eyes. Under optimal conditions, the visual detection limit is 0.72 ng mL−1, which demonstrated the high sensitivity of this assay. The stability test indicated that the immunochromatographic strips could be used within 7 weeks at room temperature without significant loss of activity. Spiked pork samples were detected by ICA and confirmed by enzyme-linked immunosorbent assay (ELISA). The assay time for SEM detection was within 5 min, suitable for a rapid on-site testing for meat samples.
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Draisci R, Giannetti L, Lucentini L, Palleschi L, Brambilla G, Serpe L, Gallo P (1997) J Chromatogr A 777:201–211
De la Calle MB, Anklam E (2005) Anal Bioanal Chem 382:968–977
Joint Expert Committee on Food Additives (JECFA) (2006) Monographs and Evaluations web site. URL: http://www.inchem.org/documents/jecfa/jecmono/v31je07.htm
McCracken RJ, Blanchflower WJ, Rowan C, McCoy MA, Kennedy DG (1995) Analyst 120:2347–2351
Nouws JFM, Laurensen J (1990) Vet Q 12:56–59
Gottschall DW, Wang R (1995) J Agric Food Chem 43:2520–2525
Hoogenboom LAP, Tomassini O, Oorsprong MBM, Kuiper HA (1991) Food Chem Toxicol 29:185–191
McCracken RJ, Kennedy DG (1997) J Chromatogr A 771:349–354
Mori K, Yasuno A, Matsumoto K (1960) Gann 51:83–89
Toth B, Shumizu H, Erickson J (1975) Eur J Cancer 11:17–22
Weisburger EK, Ulland BM, Nam J, Gart JJ, Weisburger JH, Natl J (1981) Cancer Inst 67:75–88
Pereira AS, Donato JL, De Nucci G (2004) Food Addit Contam 21:63–69
Noonan GO, Warner CR, Hsu W, Begley TH, Perfetti GA, Diachenko GW (2005) J Agric Food Chem 53:4680–4685
Becalski A, Lau BPY, Lewis D, Seaman SW (2006) Food Addit Contam 23:107–109
De Souza SVC, Junqueira RG, Ginn R (2005) J Chromatogr A 1077:151–158
Keeffe MO, Conneely A, Cooper KM, Kennedy DG, Kovacsics L, Mulder J, Van Rhijn JA, Trigueros G (2004) Anal Chim Acta 520:125–131
Saari L, Peltonen K (2004) Food Addit Contam 21:825–832
Cooper KM, Samsonova JV, Plumpton L, Elliott CT, Kennedy DG (2007) Anal Chim Acta 592:64–71
Gao AZ, Chen QL, Cheng Y, Lei J, Zeng LW (2007) Anal Chim Acta 592:58–63
Vass M, Diblikova I, Cernoch I, Franek M (2008) Anal Chim Acta 608:86–94
Adanyi N, Varadi M, Kim N, Szedro I (2006) Curr Appl Phys 6:279–286
Shi CG, Zhao SQ, Zhang K, Hong GB, Zhu ZY (2008) J Environ Sci 20:1392–1397
Xu YP, Liu LQ, Li QS, Peng CF, Chen W, Xu CL (2008) Biomed Chromatogr 23:308–314
Frens G (1973) Nat Phys Sci 241:20–22
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We are grateful to the financial support of the Science and Technology Plan Foundation of Guangdong Province (2005B20401008).
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Jinting Xu is co-first author.
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Tang, Y., Xu, J., Wang, W. et al. A sensitive immunochromatographic assay using colloidal gold–antibody probe for the rapid detection of semicarbazide in meat specimens. Eur Food Res Technol 232, 9–16 (2011). https://doi.org/10.1007/s00217-010-1351-2
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DOI: https://doi.org/10.1007/s00217-010-1351-2