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Development of colloidal carbon-based immunochromatographic strip for rapid detection of carbaryl in fruit juices

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Abstract

A simple immunochromatographic assay for sensitive detection of insecticide carbaryl in fruit juices was developed. The test is based on inhibition format on a nitrocellulose membrane strip. The strip was separately coated with rabbit anti-swine antibody (control line) and carbaryl hapten-OVA conjugate (test line). Colour intensity on the test line was possible to recognize visually from that of negative sample within 10 min, with the detection limit of 5 ng mL−1. All characteristics of the visually evaluated strips were also measured quantitatively, and then the detection limit was 1.5 ng mL−1. Cross-reactions with other carbamate pesticides were not found (<1%). No matrix effects were observed when fruit juices (orange, apple, pear, banana) were diluted tenfold times before analyses. The results from immunochromatographical assay were in a good agreement with those obtained by enzyme-linked immunosorbent assay as reference method. All these facts indicate the potential of the immunochromatographic strip for the quality control of fruit juices.

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References

  1. González-Martinez MA, Morais S, Puchades R, Maquieira A, Abad A, Montoya A (1997) Anal Chem 69:2812–2818

    Article  Google Scholar 

  2. Abad A, Moreno MJ, Pelegri R, Martinez MI, Sáez A, Gamón M, Montoya A (2001) J Agric Food Chem 49:1707–1712

    Article  CAS  Google Scholar 

  3. Commission Regulation (EC) No 149/2008 of 29 January 2008 amending Regulation (EC) No 396/2005 of the European Parliament and of the Council by establishing Annexes II, III and IV setting maximum residue levels for products covered by Annex I thereto

  4. Santalad A, Srijaranai S, Burakham R, Glennon JD, Deming RL (2009) Anal Bioanal Chem 394:1307–1317

    Article  CAS  Google Scholar 

  5. Topuz S, Ozhan G, Alpertunga B (2005) Food Control 16:87–92

    Article  CAS  Google Scholar 

  6. Fu LY, Liu XJ, Hu J, Zhao XN, Wang HL (2009) Anal Chim Acta 632:289–295

    Article  CAS  Google Scholar 

  7. Huertas-Perez JF, Garcia-Campana AM (2008) Anal Chim Acta 630:194–204

    Article  CAS  Google Scholar 

  8. Zrostlikova J, Hajslova J, Kovalczuk T, Stepan R, Poustka J (2003) J AOAC Int 86:612–622

    CAS  Google Scholar 

  9. Abad A, Montoya A (1997) J Agric Food Chem 45:1495–1501

    Article  CAS  Google Scholar 

  10. Wang S, Yu C, Wang J (2005) Food Addit Contam 22:735–742

    Article  CAS  Google Scholar 

  11. Zhang C, Ma GP, Fang GZ, Zhang Y, Wang S (2008) J Agric Food Chem 56:8832–8837

    Article  CAS  Google Scholar 

  12. Kido H, Maquieira A, Hammock BD (2000) Anal Chim Acta 411:1–11

    Article  CAS  Google Scholar 

  13. March C, Manclus JJ, Jimenez Y, Arnau A, Montoya A (2009) Talanta 78:827–833

    Article  CAS  Google Scholar 

  14. Mauriz E, Calle A, Abad A, Montoya A, Hildebrandt A, Barcelo D, Lechuga LM (2006) Biosens Bioelectron 21:2129–2136

    CAS  Google Scholar 

  15. Mauriz E, Calle A, Montoya A, Lechuga LM (2006) Talanta 69:359–364

    Article  CAS  Google Scholar 

  16. Zhang C, Ma GP, Fang GZ, Zhang Y, Wang S (2008) J Agric Food Chem 56:8832–8837

    Article  CAS  Google Scholar 

  17. Kolosova AY, Sibanda L, Dumoulin F, Lewis J, Duveiller E, Van Peteghem C, De Saeger S (2008) Anal Chim Acta 616:235–244

    Article  CAS  Google Scholar 

  18. Blažková M, Koets M, Rauch P, Van Amerongen A (2009) Eur Food Res Technol 229:867–874

    Article  Google Scholar 

  19. Blažková M, Karamonová L, Greifová M, Fukal L, Hoza I, Rauch P, Wyatt G (2006) Eur Food Res Technol 223:821–827

    Article  Google Scholar 

  20. Rusanova TY, Beloglazova NV, Goryacheva IY, Lobeau M, Van Peteghem C, De Saeger S (2009) Anal Chim Acta 653:97–102

    Article  CAS  Google Scholar 

  21. Posthuma-Trumpie GA, Korf J, Van Amerongen A (2009) Anal Bioanal Chem 393:569–582

    Article  CAS  Google Scholar 

  22. Blažková M, Mičková-Holubová B, Rauch P, Fukal L (2009) Biosens Bioelectron 25:753–758

    Article  Google Scholar 

  23. Wang S, Zhang C, Wang J, Zhang Y (2005) Anal Chim Acta 546:161–166

    Article  CAS  Google Scholar 

  24. Wang S, Zhang C, Zhang Y (2005) Anal Chim Acta 535:219–225

    Article  CAS  Google Scholar 

  25. Zhang C, Zhang Y, Wang SJ (2006) Agric Food Chem 54:2502–2507

    Article  CAS  Google Scholar 

  26. Mickova B, Zrostlikova J, Hajslova J, Rauch P, Moreno MJ, Abad A, Montoya A (2003) Anal Chem Acta 495:123–132

    Article  CAS  Google Scholar 

  27. Mickova B, Kovalczuk T, Rauch P, Moreno MJ, Abad A, Montoya A, Ferri E, Fini F, Girotti S (2005) Anal Chim Acta 528:243

    Article  CAS  Google Scholar 

  28. Van Amerongen A, Wichers JH, Berendsenm LBJM, Timmermans AJM, Keizer GD, Van Doorn AWJ, Bantjes A, Van Gelder WMJ (1993) J Biotechnol 30:185–195

    Article  Google Scholar 

  29. O′Keeffe M, Crabbe P, Salden M, Wichers JH, Van Peteghem C, Kohen F, Pieraccini G, Moneti G (2003) J Immunol Methods 278:117–126

    Article  Google Scholar 

Download references

Acknowledgments

The authors are grateful to financial support from the Grant Agency of the Czech Republic (project No. 525/07/P273) and to Ministry of Education of the Czech Republic (project No. MSMT 6046137305).

Conflict of interest statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Technicka 5, 166 28, Prague 6, Czech Republic

    Barbora Holubová-Mičková, Martina Blažková, Ladislav Fukal & Pavel Rauch

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  1. Barbora Holubová-Mičková
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  2. Martina Blažková
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  3. Ladislav Fukal
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  4. Pavel Rauch
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Correspondence to Pavel Rauch.

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Holubová-Mičková, B., Blažková, M., Fukal, L. et al. Development of colloidal carbon-based immunochromatographic strip for rapid detection of carbaryl in fruit juices. Eur Food Res Technol 231, 467–473 (2010). https://doi.org/10.1007/s00217-010-1301-z

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  • DOI: https://doi.org/10.1007/s00217-010-1301-z

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