Abstract
Wholegrain barley and peeled and pearled barley were roller milled in a Bühler mill, each producing three fractions: endosperm, middlings and bran. These fractions were compared and contrasted with one another and also with milled wheat fractions. Barley endosperm had the highest total starch content in both wholegrain and peeled and pearled barley. Amylose-to-amylopectin ratios (Am:Ap) were higher in barley flours than in wheat flours (P < 0.001). Peeled and pearled barley grain had lower soluble and insoluble fibre contents in all fractions, with wholegrain barley bran in particular having significantly more fibre than that of peeled and pearled barley bran (P < 0.001). The protein contents of the fractions of both barley types did not differ significantly. The endosperm had the lowest protein content, with the bran and middling fractions having double the protein content of the endosperm fraction. The total amino acid concentrations of the fractions followed a similar pattern, the total amino acid concentrations being lower in the endosperm fractions than in the bran and in the middlings fractions. These findings suggest that both types of barley fractions differed essentially from those of wheat. Pearling has a greater effect on the middlings and bran fractions than on the endosperm. The majority of these differences may not be significant enough to impact on the baking qualities of breads made using these fractions.
Similar content being viewed by others
References
Feldheim W, Wisker E (2000) Studies on the improvement of dietary fibre intake. Dtsch Lebensm-Rundsch 96:327–330
Wood PJ, Braaten JT, Fraser WS, Riedel D, Poslel M (1990) Comparisons of viscous properties of oat and guar gum and effects of these and oat bran on glycemic index. J Agric Food Chem 38:753–757
Wood PJ (1993) Physicochemical characteristics and physiological properties of oat (1-3) (1-4) β-d-glucan. In: Wood PJ (ed) Oat bran. AOAC, St Paul, MN, pp 83–112
Bourdon I, Yokoyama W, Davis P, Hudson C, Backus R, Richter D, Knuckles B, Schneeman BO (1999) Postprandial lipid, glucose, insulin, and cholecystokinin responses in men fed barley pasta enriched with β-glucan. Am J Clin Nutr 69:55–63
Sundberg B, Pettersson D, Åman P (1995) Nutritional properties of fibre-rich barley products fed to broiler chickens. J Sci Food Agric 67:469–476
Fukuda M, Kanauchi O, Araki Y, Andoh A, Mitsuyama K, Takagi K (2002) Prebiotic treatment of experimental colitis with germinated barley foodstuff: A comparison with probiotic or antibiotic treatment. Int J Mol Med 9:65–70
Skendi A, Papageorgiou M, Biliaderis CG (2009) Effect of barley β-glucan molecular size and level on wheat dough rheological properties. J Food Eng 91:594–601
Banchathanakij R, Suphantharika M (2009) Effect of different β-glucans on the gelatinisation and retrogradation of rice starch. Food Chem 114:5–14
Baik B-K, Ullrich SE (2008) Barley for food: characteristics, improvement, and renewed interest. J Cereal Sci 48(2):233–242
Skendi A, Biliaderis CG, Lazaridou A, Izydorczyk MS (2003) Structure and rheological properties of water soluble β-glucans from oat cultivars of Avena sativa and Avena bysantina. J Cereal Sci 38:15–31
FDA (1997) Food labeling: soluble dietary fibre from certain foods and coronary heart disease. Federal Regist 67:61773–61783
FDA (2005) Food labelling: soluble dietary fibre from certain foods and coronary heart disease. Federal Regist 70:76150–76162
FDA (2008) Food labeling: health claims; soluble fiber from certain foods and risk of coronary heart disease. Interim final rule. Federal Regist 73(37):9938–9947
Ames NP, Rhymer CR (2008) Issues surrounding health claims for barley. J Nutr 138(6):1237S–1243S
Izydorczyk MS, Jacobs M, Dexter JE (2003) Distribution and structural variation of non-starch polysaccharides in milling fractions of hull-less barley with variable amylose content. Cereal Chem 80:645–653
Knuckles BE, Chiu MM (1995) β-Glucan-enrichment of barley fractions by air classification and sieving. J Food Sci 60:1070–1074
Knuckles BE, Chiu MM, Betschart AA (1992) β-Glucan-enriched fractions from laboratory-scale dry milling and sieving of barley and oats. Cereal Chem 69:198–202
AACC (1988) Approved methods of the AACC, 8th edn. Method 26–20, approved April 1961 revised December 1988. The American Association of Cereal Chemists, St. Paul, MN, USA
International Standard (1987) Rice-determination of amylose content. (1987) (E); ISO 6647:2–3
Zhou MX, Mendham NJ (2005) Predicting barley malt extract with a Rapid ViscoAnalyser. J Cereal Sci 41:31–36
Zhou MX, Robards K, Glennie-Holmes M, Helliwell S (1998) Structure and pasting properties of oat starch. Cereal Chem 75:273–281
AOAC (2000) Method no. 991.43 (enzymatic-gravimetric method—TRIS Buffer) for total, soluble, and insoluble dietary fiber in foods
Vasanthan T, Temelli F (2008) Grain fractionation technologies for cereal beta-glucan concentration. Food Res Int 41:876–881
Seyer M-E, Gélinas P (2009) Bran characteristics and wheat performance in whole wheat bread. Int J Food Sci Technol 44:688–693
Klamczynski A, Baik BK, Czuchajowska Z (1998) Composition, microstructure, water imbibition, and thermal properties of abraded barley. Cereal Chem 75:677–685
Bhatty RS, Rossnagel BG (1998) Comparison of pearled and unpearled Canadian and Japanese barleys. Cereal Chem 75(1):15–21
Andersson A, Courtin C, Delcour J, Fredriksson H, Schofield J, Trogh I, Tsiami AA, Åman P (2003) Milling performance of north European hull-less barleys and characterization of resultant millstreams. Cereal Chem 80(6):667–673
Flores RA, Hicks KB, Eustace DW, Phillips J (2005) High-starch and high β-glucan fractions milled with experimental mills. Cereal Chem 82(6):727–733
Sundberg B, Åman P (1994) Fractionation of different types of barley by roller milling and sieving. J Cereal Sci 19(2):179–184
van Amelswoort JMM, Westrate JA (1992) Amylose–amylopectin ratio in a meal affects postprandial variables in male volunteers. Am J Clin Nutr 55:712–718
Zhang SB, Lu QY (2005) Effects of amylose content on flour pasting properties and noodle qualities. J Henan Univ Technol 26:9–12
Song JM, Liu AF, You MS, Li BY, Wu XY, Zhao ZD, Liu GT (2004) Effects of waxy flour blending on starch pasting properties and noodle quality of nonwaxy flour. Sci Agric Sin 37:1838–1842
Ragaee S, Abdel-Aal E-SM (2006) Pasting properties of starch and protein in selected cereals and quality of their food products. Food Chem 95:9–18
AACC (2001) The definition of dietary fiber. Cereal Foods World 46(3):112–126
Marlett J (1991) Dietary fibre content and effect of processing on two barley varieties. Cereal Foods World 36:576–578
Fastnaught CE (2001) Barley fibre. In: Cho S, Dreher M (eds) Handbook of dietary fibre. Marcel Dekker, New York, pp 519–542
Fastnaught CE, Berglund PT, Holm ET, Fox GJ (1996) Genetic and environmental variation in β-glucan content and quality parameters of barley for food. Crop Sci 36:941–946
Marconi E, Graziano M, Cubadda R (2000) Composition and utilization of barley pearling by-products for making functional pasta rich in dietary fibre and β-glucans. Cereal Chem 77:133–139
Virkki L, Johansson L, Ylinen M, Manau S, Ekholm P (2004) Structural characterization of water-insoluble nonstarchy polysaccharides of oats and barley. Carbohydr Polym 59:357–366
Zhang G, Junmei W, Jinxin C (2002) Analysis of β-glucan content in barley cultivars from different locations of China. Food Chem 79:251–254
Macgregor AW, Fincher GB (1993) Carbohydrates of the barley grain. In: Macgregor AW, Bhatty RS (eds), Barley: chemistry and technology. American Association of Cereal Chemistry, St Paul MN, 73–130
Panfili G, Fratianni A, Di Criscio T, Marconi E (2008) Tocol and β-glucan levels in barley varieties and in pearling by-products. Food Chem 107:84–91
Faulks AJ, Shewry PR, Miflin BJ (1981) The polymorphism and structural homology of storage polypeptides (hordein) coded by the Hot 2 locus in barley (Hordeuni vulgare L.). Biochem Genet 19:841–858
Shewry PR, Kreis M, Parmar S, Lew EJ-L, Kasarda DD (1985) Identification of γ-type hordeins in barley. Fed Eur Biochem Sci Lett 190(1):61–64
Shewry PR, Finch RA, Parmar S, Franklin J, Miflin BJ (1983) Heredity 50(2):179–189
Qi J-C, Zhang G-P, Zhou M-X (2006) Protein and hordein content in barley seeds as affected by nitrogen level and their relationship to beta-amylase activity. J Cereal Sci 43:102–107
Salcedo G, Sanchez-Monge R, Argamenteria A, Aragoncillo C (1980) The A-hordeins as a group of salt soluble hydrophobic proteins. Plant Sci Lett 19:109–119
Ullrich SE, Kleinhofs A, Coon CN, Nilan RA (1984) Breeding for improved protein in barley. In: Cereal grain protein improvement. International Atomic Agency, Vienna, pp 93–104
Bang-Olsen K, Stilling B, Munck L (1987) Breeding for yield in high lysine barley. In: Yasuda S, Konishi T (eds) Barley genetics V. In: Proceedings of the 5th international barley genetic symposium. Sanyo Press Co. Ltd., Okayama, pp 865–870
Ullrich SE, Eslick RF (1978) Inheritance of the associated kernel characters—high lysine and shrunken endosperm of the barley mutant Bomi, Riso 1508. Crop Sci 18:828–831
Ullrich SE, Eslick RF (1978) Lysine and protein characterization of induced shrunken endosperm mutants of barley. Crop Sci 18:963–966
Svihus B, Newman CW, Newman RK, Selmer-Olsen I (1997) Changes in extract viscosity, amino acid content, and soluble and insoluble β-glucan and dietary fibre content of barley during different high moisture storage conditions. Anim Feed Sci Technol 64:257–272
Di Giuseppe M (ed) (2003) Nelson Biology 12. Thomson Canada Ltd., Toronto, pp 473
Vasanthan T, Bhatty RS (1996) Physicochemical properties of small- and large-granule starches of waxy, regular and high amylose barleys. Cereal Chem 73:199–207
Peng M, Gao M, Abdel-Aal ESM, Hucl P, Chibbar RN (1999) Separation and characterization of A and B type starch granules in wheat endosperm. Cereal Chem 76:375–379
Izydorczyk MS, Lagassé SL, Hatcher DW, Dexter JE, Rossnagel BG (2005) The enrichment of Asian noodles with a fiber-rich fraction derived from roller milling of hull-less barley. J Sci Food Agric 85(12):2094–2104
Acknowledgments
This project is funded by the Department of Agriculture, Fisheries and Food under the Food Institutional Research Measure. The authors would also like to acknowledge the staff at the National Food Imaging Centre, Moorepark, Cork, Ireland for their assistance in the analysis of the fractions by scanning electron microscopy and Cäcilia Spoerndli from Bühler Group for milling and pearling assistance.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Sullivan, P., O’Flaherty, J., Brunton, N. et al. Chemical composition and microstructure of milled barley fractions. Eur Food Res Technol 230, 579–595 (2010). https://doi.org/10.1007/s00217-009-1196-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00217-009-1196-8