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Probing the sol–gel transition of egg white proteins by pulsed-NMR method

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Abstract

The utility of pulsed NMR spectroscopy as a convenient means to monitor a sol–gel transition during heat-induced gelation of egg white proteins was investigated. This is based on the notion that, regardless of the mechanism of gel formation, the transition from sol to a gel state in such system is accompanied by the appearance of solid-like domain and a concomitant increase in the NMR signal attributed to protons in the solid-like fraction. The transition from sol to a gel state was clearly evident when the egg white was held isothermally at 80 °C in a water bath. The effects of some sugars and chemical cross-linking agents were also investigated in order to establish whether the method is capable of differentiating the relative effect of the chemicals on the kinetics of the gelation process. The results showed that pulsed NMR has a potential value as a complementary method to study sol–gel transition of food biopolymer.

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Abbreviations

S%:

A measure of solid-like component

R 11 :

Signal at 11 μs, which is proportional to the total content of protons in the sample

R 70 :

Signal at 70 μs, which is proportional to the amount of protons in the liquid phase of the sample

References

  1. Ablett S (1992) Trends Food Sci Technol 3:246–250

    Article  CAS  Google Scholar 

  2. Teo CH, Seow CC (1992) Starch/Starke 44:288–292

    Article  CAS  Google Scholar 

  3. Van Duynhoven JPM, Kulik AS, Jonker HRA, Haverkamp J (1999) Carbohydr Polym 40:211–219

    Article  Google Scholar 

  4. Karim AA, Norziah MH, Seow CC (2000) Food Chem 71:9–36

    Article  CAS  Google Scholar 

  5. Ando I, Kuroki S, Kobayashi M, Zhao C, Matsukawa S (2002) In: Osada Y, Khokhlov AR (eds) Polymer gels and networks. Marcel Dekker, Inc, USA, pp 235–307

  6. Zandi M, Mirzadeh H, Mayer C (2007) Eur Polym J 43:1480–1486

    Article  CAS  Google Scholar 

  7. Shimada K, Matsushita S (1980) J Agric Food Chem 28:409–412

    Article  CAS  Google Scholar 

  8. Armstrong HJ, Hill SE, Schrooyen P, Mitchell JR (1994) J Texture Stud 25:285–298

    Article  Google Scholar 

  9. Kulmyrzaev A, Bryant C, McClements DJ (2000) J Agric Food Chem 48:1593–1597

    Article  CAS  Google Scholar 

  10. Hill S, Easa AM (1998) In: O’Brien J, Nursten HE, Crabbe MJC, Ames JM (eds) The Maillard reaction in food and medicine. The Royal Society of Chemistry, UK, pp 133–138

  11. Easa AM, Armstrong HJ, Mitchell JR, Hill SE (1996) Int J Biol Macromol 18:297–301

    Article  CAS  Google Scholar 

  12. Fayle SE, Gerrard JA, Simmons L, Meade SJ, Reid EA, Johnston AC (2000) Food Chem 70:193–198

    Article  CAS  Google Scholar 

  13. Wong SS (1993) Chemistry of protein conjugation and cross-linking. CRC Press, LLC, USA, pp 75–145

    Google Scholar 

  14. Gerrard JA, Brown PK, Fayle SE (2003) Food Chem 80:35–43

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by an 8th Malaysia Plan R&D grant under the Fundamental Research Grant Scheme of the Ministry of Science, Technology and Environment, Malaysia.

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Authors and Affiliations

  1. Food Biopolymer Science Research Group, School of Industrial Technology, Universiti Sains Malaysia, 11800, Minden, Penang, Malaysia

    K. S. Goh, Rajeev Bhat & A. A. Karim

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  1. K. S. Goh
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  2. Rajeev Bhat
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  3. A. A. Karim
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Correspondence to A. A. Karim.

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Goh, K.S., Bhat, R. & Karim, A.A. Probing the sol–gel transition of egg white proteins by pulsed-NMR method. Eur Food Res Technol 228, 367–371 (2009). https://doi.org/10.1007/s00217-008-0942-7

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  • DOI: https://doi.org/10.1007/s00217-008-0942-7

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