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Antimicrobial activity of recombinant human lactoferrin from Aspergillus awamori, human milk lactoferrin and their hydrolysates

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Abstract

The antibacterial activity of human lactoferrin from milk (hLF), recombinant human lactoferrin from Aspergillus awamori (rhLF) and their hydrolysates obtained with pepsin was investigated against Escherichia coli O157:H7, Salmonella Enteritidis and Listeria monocytogenes. The minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) were determined for all the bacteria and the proteins assayed. Taking into account the MICs found for both lactoferrins studied, we can say that they behave very similarly, except for L. monocytogenes for which rhLF was more active. We studied the effect that heat treatments exerted on the antibacterial activity of the two types of lactoferrin and the only heat treatment that had a negative effect on that activity was 85 °C for 10 min. The activity of hLF and rhLF in UHT milk and whey against E. coli O157:H7 and L. monocytogenes, was also assayed. Our results showed a reduction in the number of viable cells for both microorganisms when were incubated with rhLF or hLF, but this decrease was lower than in broth media.

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Acknowledgments

We thank Agennix Inc. (Houston, TX, USA) for donating the recombinant human lactoferrin and supporting the performance of this work. This work was supported by a CICYT Grant (AGL 2005-05494) from the Ministerio de Ciencia y Tecnología and by a research-fellow grant from Gobierno de Aragón.

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Authors and Affiliations

  1. Facultad de Veterinaria, Tecnología de los Alimentos, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain

    Celia Conesa, María-Dolores Pérez, Miguel Calvo & Lourdes Sánchez

  2. Facultad de Veterinaria, Nutrición y Bromatología, Universidad de Zaragoza, Miguel Servet 177, 50013, Zaragoza, Spain

    María Carmen Rota

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  1. Celia Conesa
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  2. María Carmen Rota
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  3. María-Dolores Pérez
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  4. Miguel Calvo
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  5. Lourdes Sánchez
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Correspondence to Lourdes Sánchez.

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Conesa, C., Rota, M.C., Pérez, MD. et al. Antimicrobial activity of recombinant human lactoferrin from Aspergillus awamori, human milk lactoferrin and their hydrolysates. Eur Food Res Technol 228, 205–211 (2008). https://doi.org/10.1007/s00217-008-0924-9

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  • DOI: https://doi.org/10.1007/s00217-008-0924-9

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