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Improved lycopene extraction from tomato peels using cell-wall degrading enzymes

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Abstract

Four commercial enzyme preparations with pectinolytic, cellulolytic and hemicellulolytic activities were tested for their ability to enhance lycopene extraction from tomato peels. Screening experiments were performed at 25 °C by subjecting the peels to a 4-h enzyme incubation followed by 1-h hexane extraction. Peclyve EP and LI were the most efficient, with an almost 20-fold increase in extraction yield. Peclyve LI was used to evaluate the influence of solvent type and enzyme incubation time on lycopene recovery. Hexane, ethyl acetate and the mixture hexane/acetone/ethanol 50:25:25 (v/v) were used as solvents. Under the best extraction conditions (1-h enzyme incubation followed by a 3-h solvent extraction at 40 °C) up to 440 mg of lycopene per 100 g of dry tomato peels were obtained. The percentage recoveries were in the range of 3–30%, for the untreated peels, and 77–98% for the enzymatically treated material.

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Acknowledgments

We thank NOVOZYMES A/S (Bagsvaerd, Denmark) and LYVEN S.A. (Colombelles, France) for their kind gift of enzyme preparations.

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Authors and Affiliations

  1. Department of Chemical Engineering, Materials and Environment, Sapienza University of Rome, Via Eudossiana 18, 00184, Rome, Italy

    Roberto Lavecchia & Antonio Zuorro

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  1. Roberto Lavecchia
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  2. Antonio Zuorro
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Correspondence to Roberto Lavecchia.

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Lavecchia, R., Zuorro, A. Improved lycopene extraction from tomato peels using cell-wall degrading enzymes. Eur Food Res Technol 228, 153–158 (2008). https://doi.org/10.1007/s00217-008-0897-8

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  • DOI: https://doi.org/10.1007/s00217-008-0897-8

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