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Construction of proteinase A deficient transformant of industrial brewing yeast

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Abstract

Yeast proteinase A is detrimental to beer foam. The proteinase A deficient transformant of industrial brewing yeast, WZ65/a, was constructed using PCR-mediated gene disruption, and the transformant was verified to be genetically stable. The PCR analysis showed that PEP4 gene coding for proteinase A in the WZ65/a was disrupted. No matter in the yeast cells or in the fermenting liquor of WZ65/a, proteinase A activity could not be detected. Analysis of the main charicteristics indexes of beer also showed that proteinase A activity and foam performance in the beer brewed with WZ65/a were better than that of the host strain, WZ65.

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Acknowledgements

We appreciate the financial support by “Natural Science Fundation of Zhejiang Province” for this work (No. Z304076).

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Authors and Affiliations

  1. Department of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, P.R. China

    Zhao-Yue Wang, Guo-Qing He, Hui Ruan & Lu-Fang Yang

  2. China Lion Brewery Group, Wenzhou, P.R. China

    Zhong-Shan Liu

  3. The Lab of Breeding and Molecular Genetics of Yeast, Institute of Microbiology, Chinese Academy of Sciences, Beijing, P.R. China

    Zhao-Yue Wang & Bo-Run Zhang

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  1. Zhao-Yue Wang
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  2. Guo-Qing He
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  3. Hui Ruan
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  4. Zhong-Shan Liu
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  5. Lu-Fang Yang
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Correspondence to Guo-Qing He.

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Wang, ZY., He, GQ., Ruan, H. et al. Construction of proteinase A deficient transformant of industrial brewing yeast. Eur Food Res Technol 225, 831–835 (2007). https://doi.org/10.1007/s00217-006-0488-5

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  • DOI: https://doi.org/10.1007/s00217-006-0488-5

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