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Antioxidant activity of effluents during the purification of hydroxytyrosol and 3,4-dihydroxyphenyl glycol from olive oil waste

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Abstract

Two different processes have been developed for the recovery of hydroxytyrosol (HT) and 3,4-dihydroxyphenyl glycol (DHPG) from olive oil mills waste. The antioxidant activity of several effluents has been characterised by four “in vitro” tests: antiradical capacity (ARC), ferric reducing power (P R) and inhibition of primary and secondary oxidation in lipid systems. HT-containing effluents exhibited higher or similar activity in ARC and P R than equimolecular quantities of vitamins E and C. In oxidative tests they were between both assayed vitamins. Except for commercial Hytolive®2, activities correlated with HT concentration of each sample. DHPG-containing effluent had higher activities than a solution with the same concentration of the synthetic DL-DHPG. This effluent was the most active in ARC and P R tests, its activity being similar to vitamin E in the inhibition of primary oxidation. Both phenolic compounds showed antagonist effect when they coeluted, except for P R assay. These results suggested that due to the low-cost procedure and to the antioxidant activity of effluents, they could be valuable as natural additives for food, pharmaceutical and cosmetic industries.

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Abbreviations

HT:

hydroxytyrosol

DHPG:

3,4-dihydroxyphenyl glycol

DPPH:

2,2-diphenyl-1-picrylhydrazyl (free radical)

ABAP:

2,2′-azobis(2-amidinopropane) dihydrochloride

SDS:

sodium dodecylsulphate

RT:

retention time

EC50 :

amount of antioxidant necessary to decrease the initial absorbance by 50%

EC25 :

amount of antioxidant necessary to decrease the initial absorbance by 25%

ARC:

antiradical capacity

P R :

reducing power

QE:

quercetin equivalent

POIC:

primary oxidation inhibition capacity

TBARS:

thiobarbituric acid reactive species

R :

correlation coefficient

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Acknowledgments

The authors thank GENOSA for the supply of Hytolive®2. Financial support: Junta de Andalucía, Consejería de Innovación, Ciencia y Empresa (projects CAO01-006 and CO3-055).

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Authors and Affiliations

  1. Instituto de la Grasa, C.S.I.C., Avenida Padre García Tejero no. 4, 41012, Sevilla, Spain

    Guillermo Rodríguez, Rocío Rodríguez, Juan Fernández-Bolaños, Rafael Guillén & Ana Jiménez

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  1. Guillermo Rodríguez
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  2. Rocío Rodríguez
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  3. Juan Fernández-Bolaños
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  4. Rafael Guillén
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  5. Ana Jiménez
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Correspondence to Ana Jiménez.

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Rodríguez, G., Rodríguez, R., Fernández-Bolaños, J. et al. Antioxidant activity of effluents during the purification of hydroxytyrosol and 3,4-dihydroxyphenyl glycol from olive oil waste. Eur Food Res Technol 224, 733–741 (2007). https://doi.org/10.1007/s00217-006-0366-1

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  • DOI: https://doi.org/10.1007/s00217-006-0366-1

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