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Bread quality and dough rheology of enzyme-supplemented wheat flour

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Abstract

The enzymatic treatment of wheat flours is an interesting alternative for improving their functional properties. Since enzymes with different biochemical activities could induce synergistic effects on dough behaviour or product quality, the individual and combined use of a wide range of enzymes (transglutaminase, glucose oxidase, laccase, α-amylase, pentosanase and protease) applied nowadays in bread-making processes were investigated. The blend of enzymes resulted in an improvement in the rheological behaviour of doughs and the quality of the final product. The simultaneous presence of transglutaminase (TG) and glucose oxidase (GO), as well as TG and protease (PROT) led to a synergistic effect on alveograph parameters. Polysaccharide-degrading enzymes exercised a significant effect on rheology only when used in combination with other enzymes, mainly affecting consistograph parameters. Analysis of bread-making data revealed significant interactions between TG and all the other enzymes except laccase (LAC). Significant synergistic effect on bread quality was observed by the combined use of GO and LAC, GO and pentosanase (PP), amylase (AMYL) and LAC, AMYL and PROT, and PP and PROT. Bread quality parameters showed greater correlations with alveograph parameters than with consistograph properties of dough. Tenacity (P) and extensibility (L) proved to be acceptable predictors of the height/width ratio of loaves. The duration of the alveograph test enhanced the prediction of bread quality parameters. Conversely, none of the rheological properties studied showed a high correlation with the specific volume of loaves.

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References

  1. Blumenthal CS, Barlow EWR, Wrigley CW (1993) J Cereal Sci 18:3–21

    Article  CAS  Google Scholar 

  2. Perrotta C, Treglia AS, Mita G, Giangrande E, Rampino P, Ronga G, Spano G, Marmiroli N (1998) J Cereal Sci 27:127–132

    Article  CAS  Google Scholar 

  3. Iriki N, Yamauchi H, Takata K, Nishio Z, Ichinose Y, Yoshihira T (2003) Food Sci Technol Res 9:104–109

    Google Scholar 

  4. Fisher N, Hutchinson JB, Berry R, Hardy J, Ginocchio AV, Waite Y (1979) Food Cosmet Toxicol 17:33–39

    Article  CAS  Google Scholar 

  5. Kurokawa Y, Maekawa A, Takahashi M, Hayashi Y (1990) Environ Health Perspect 87:309–335

    CAS  Google Scholar 

  6. Dupuis B (1997) Cereal Foods World 42:171–183

    CAS  Google Scholar 

  7. Wolf DC, Crosby LM, George MH, Kilbur SR, Moore TM, Miller RT, DeAngelo AB (1998) Toxicol Pathol 26:724–729

    Article  CAS  Google Scholar 

  8. Stauffer CE (1990) Functional additives for bakery foods. Van Nostrand Reinhold, New York

  9. Gerrard JA (2002) Trends Food Sci Technol 13:389–397

    Article  CAS  Google Scholar 

  10. Motoki M, Seguro K (1998) Trends Food Sci Technol 9:204–210

    Article  CAS  Google Scholar 

  11. Gerrard JA, Fayle SE, Brown PA, Sutton KH, Simmons L, Rasiah I (2001) J Food Sci 66:782–786

    Article  CAS  Google Scholar 

  12. Larré C, Denery PS, Popineau Y, Deshayes G, Desserme C, Lefevre J (2000) Cereal Chem 77:32–38

    Google Scholar 

  13. Mujoo R, Ng PKW (2003) Cereal Chem 80(6):703–706

    CAS  Google Scholar 

  14. Bauer N, Koehler P, Wieser H, Schieberle P (2003) Cereal Chem 80:781–786

    CAS  Google Scholar 

  15. Rosell CM, Wang J, Aja S, Bean S, Lookhart G (2003) Cereal Chem 80:52–55

    CAS  Google Scholar 

  16. Gujral HS, Rosell CM (2004) J Cereal Sci 39:225–230

    Article  CAS  Google Scholar 

  17. Gerrard JA, Fayle SE, Wilson AJ, Newberry MP, Ross M, Kavale S (1998) J Food Sci 63:472–475

    Article  CAS  Google Scholar 

  18. Basman A, Köksel H, Perry KWN (2002) Eur Food Res Technol 215:419–424

    Article  CAS  Google Scholar 

  19. Tseng CS, Lai HM (2002) J Food Sci 67:750–755

    Article  CAS  Google Scholar 

  20. Bauer N, Koehler P, Wieser H, Schieberle P (2003) Cereal Chem 80:787–790

    CAS  Google Scholar 

  21. Autio K, Kruus K, Knaapila A, Gerber N, Flander L, Buchert J (2005) J Agric Food Chem 53:1039–1045

    Article  CAS  Google Scholar 

  22. Gerrard JA, Newberry MP, Ross M, Wilson AJ, Fayle SE, Kavale S (2000) J Food Sci 65:312–314

    Article  CAS  Google Scholar 

  23. Bonet A, Caballero PA, Gomez M, Rosell CM (2005) Cereal Chem 82:425–430

    CAS  Google Scholar 

  24. Caballero PA, Bonet A, Rosell CM, Gomez M (2000) J Cereal Sci 42:93–100

    Article  CAS  Google Scholar 

  25. Köksel H, Sivri D, Ng PKW, Steffe JF (2001) Cereal Chem 78:26–30

    Google Scholar 

  26. Gujral HS, Rosell CM (2004) Food Res Int 37:75–81

    Article  CAS  Google Scholar 

  27. Gerrard JA, Sutton KH (2005) Trends Food Sci Technol 16(11):510–512

    Article  CAS  Google Scholar 

  28. Rakotozafy L, Mackova B, Delcros JF, Boussard A, Davidou S, Potus J, Nicolas J (1999) Cereal Chem 76:213–218

    CAS  Google Scholar 

  29. Hoseney RC, Faubion JM (1981) Cereal Chem 58:421–424

    CAS  Google Scholar 

  30. Haarasilta S, Pullinen T, Vaisanen S, Tammersalo-Karsten I (1991) United States Patent 4,990,343

  31. Nakai K, Takami K, Yanaka N, Takasaki Y (1995) European Patent Application 0,686,348 A1

  32. Vemulapalli V, Hoseney RC (1998) Cereal Chem 75:859–862

    CAS  Google Scholar 

  33. Aja S, Wang J, Rosell CM (2003) In: Courtin CM, Veraverbeke WS, Delcour JA (eds) Recent advances in enzymes in grain processing. American Association of Cereal Chemists, St. Paul, MN, pp 101–106

  34. Primo-Martin C, Valera R, Martínez-Anaya MA (2003) J Agric Food Chem 51:4673–4679

    Article  CAS  Google Scholar 

  35. Ameille V, Castello P, Garcia R, Rakotozafy L, Potus J, Nicolas J (2000) Sci Aliments 20(4/5):441–455

    CAS  Google Scholar 

  36. Tilley KA, Benjamin RE, Bagorogoza KE, Moses Okot-Kotber B, Praskash O, Kwen H (2001) J Agric Food Chem 49:2627–2632

    Article  CAS  Google Scholar 

  37. Rasiah IA, Sutton KH, Low FL, Lin HM, Gerrard JA (2005) Food Chem 89:325–332

    Article  CAS  Google Scholar 

  38. Martinez-Anaya MA, Jimenez T (1997) J Texture Stud 28:569–583

    Google Scholar 

  39. Vemulapalli V, Miller KA, Hoseney RC (1998) Cereal Chem 75:439–442

    CAS  Google Scholar 

  40. Wikstrom K, Eliasson AC (1998) Cereal Chem 75:331–337

    CAS  Google Scholar 

  41. Dunnewind B, Van Vliet T, Orsel R (2002) J Cereal Sci 36:357–366

    Article  CAS  Google Scholar 

  42. Xia P, Jin MG, Liang XY (1999) Food Ind 6:23–24

    Google Scholar 

  43. Garcia R, Rakotozafy L, Nicolas J (2004) J Agric Food Chem 52:3946–3953

    Article  CAS  Google Scholar 

  44. Poulsen C, Hostrup PB (1998) Cereal Chem 75:51–57

    CAS  Google Scholar 

  45. Si JQ (1994) International Patent Application WO 94/28728

  46. Figueroa-Espinoza MC, Morel MH, Rouau X (1998) J Agric Food Chem 46:2583–2589

    Article  CAS  Google Scholar 

  47. Figueroa-Espinoza MC, Morel MH, Surget A, Asther M, Moukha S, Sigoillot JC, Rouau X (1999) Food Hydrocolloids 13:65–71

    Article  CAS  Google Scholar 

  48. Labat E, Morel MH, Rouau X (2001) Food Hydrocolloids 15:47–52

    Article  CAS  Google Scholar 

  49. Oudgenoeg G, Hilhorst R, Piersma SR, Boeriu CG, Gruppen H, Hessing M, Voragen AG, Laane C (2001) J Agric Food Chem 49:2503–2510

    Article  CAS  Google Scholar 

  50. Labat E, Morel MH, Rouau X (2000) Cereal Chem 77:823–828

    CAS  Google Scholar 

  51. Primo-Martin C, Martinez-Anaya MA (2003) J Food Sci 68:31–41

    Article  CAS  Google Scholar 

  52. Gottmann K, Sproessler B (1994) European Patent Application EP 0492406, B1

  53. Bollain C, Angioloni A, Collar C (2005) Eur Food Res Technol 220:83–89

    Article  CAS  Google Scholar 

  54. Collar C, Bollain C, Angioloni A (2005) J Food Eng 70:479–488

    Google Scholar 

  55. Collar C, Bollain C (2005) Eur Food Res Technol 221:298–304

    Article  CAS  Google Scholar 

  56. AACC (2000) Approved methods of the AACC. American Association of Cereal Chemists, St. Paul, MN

    Google Scholar 

  57. Rouille J, Della Valle G, Devaux MF, Marion D, Dubreil L (2005) Cereal Chem 82:20–27

    CAS  Google Scholar 

  58. Indrani D, Prabhasankar P, Rajiv J, Venkateswara-Rao G (2003) J Food Sci 68:2804–2809

    Article  CAS  Google Scholar 

  59. Pedersen L, Kaack K, Bergsoe MN, Adler-Nissen J (2005) J Food Sci 70:152–158

    Article  Google Scholar 

  60. Bombara N, Anon MC, Pilosof AMR (1997) Lebensm Wiss Technol 30:441–447

    Article  CAS  Google Scholar 

  61. Babiker EE, Fujisawa N, Matsudomi N, Kato A (1996) J Agric Food Chem 44:3746–3750

    Article  CAS  Google Scholar 

  62. Weegels PL, Hamer RJ (1992) Cereal Foods World 37(5):379–385

    CAS  Google Scholar 

  63. Pyler EJ (1988) Baking science and technology. Sosland Publising Company, Merriam, KS

    Google Scholar 

  64. Armero E, Collar C (1997) Z Lebensm Unters Forsch 204:136–145

    Article  CAS  Google Scholar 

  65. Duran E, Leon A, Barber B, Benedito de Barber C (2001) Eur Food Res Technol 212:203–207

    Article  CAS  Google Scholar 

  66. Rouau X, Moreau D (1993) Cereal Chem 70:626–632

    CAS  Google Scholar 

  67. Collar C, Andreu P, Martinez-Anaya MA (1998) Z Lebensm Unters Forsch 207:133–139

    Article  CAS  Google Scholar 

  68. Figueroa-Espinoza MC, Rouau X (1998) Cereal Chem 75:259–265

    CAS  Google Scholar 

  69. Krishnarau L, Hoseney RC (1994) J Food Sci 59:1251–1254

    Article  CAS  Google Scholar 

  70. Blaszczak W, Sadowska J, Rosell CM, Fornal J (2004) Eur Food Res Technol 219:348–354

    CAS  Google Scholar 

  71. Gujral HS, Haros M, Rosell CM (2003) Cereal Chem 80:750–754

    CAS  Google Scholar 

  72. Primo-Martin C, Wang M, Lichtendonk WJ, Plijter JJ, Hamer RJ (2005) J Sci Food Agric 85:1186–1196

    Article  CAS  Google Scholar 

  73. Dobraszczyk BJ, Morgenstern MP (2003) J Cereal Sci 38:229–245

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was financially supported by Comisión Interministerial de Ciencia y Tecnología Projects (MCYT, AGL2002-04093-C03ALI), Consejo Superior de Investigaciones Científicas (CSIC) and Universidad de Valladolid, Spain. Authors thank Ramiro Martínez (Novo Nordisk, Madrid, Spain) for providing enzyme samples.

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Correspondence to Manuel Gómez.

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Caballero, P.A., Gómez, M. & Rosell, C.M. Bread quality and dough rheology of enzyme-supplemented wheat flour. Eur Food Res Technol 224, 525–534 (2007). https://doi.org/10.1007/s00217-006-0311-3

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