Abstract
The amino acid-decarboxylase activity of bacteria isolated from Engraulis encrasicholus preserved in ice was investigated throughout 23 days of storage. A total of 140 bacterial isolates were studied, including 37 enterobacteria, 23 pseudomonads, 49 Gram+ catalase+ cocci, 27 lactic acid bacteria and 4 enterococci. The percentage of strains that decarboxylated amino acids was low, 12% on average. None of the Gram+ catalase+ cocci showed aminogenic activity, but all enterococci isolates did. The enterobacteria with aminogenic capacity were identified as Enterobacter cloacae and they simultaneously produced putrescine and cadaverine (up to 500 mg/l), but not histamine. Among pseudomonads, two species decarboxylated ornithine (producing 350–650 mg/l putrescine): Pseudomonas cepacia and Pseudomonas fluorescens. Lactic acid bacteria decarboxylated tyrosine, yielding up to 2,000 mg/l tyramine, and they were identified as Lactobacillus brevis, Lactococcus lactis, Pediococcus pentosaceous and Enterococcus spp. As a general rule, the positive Gram-negative isolates (enterobacteria and pseudomonads) were diamine producers and they were found only during the first 2 weeks of storage. In contrast, lactic acid bacteria and enterococci were mainly tyramine producers and they were isolated only at the end of storage.
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Acknowledgements
This study was supported by grant QUALPOISS2 (FAIR.CT 96.3253). We thank the Comissió Interdepartamental de Recerca i Innovació Tecnològica (CIRIT, 2001SGR-00132) of the Generalitat de Catalunya (Spain) for financial support.
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Part of this study was presented at the “XIII Congreso de Microbiología de los Alimentos” held in Bilbao (Spain), 17–19 September 2002.
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Pons-Sánchez-Cascado, S., Bover-Cid, S., Veciana-Nogués, M.T. et al. Amino acid-decarboxylase activity of bacteria isolated from ice-preserved anchovies. Eur Food Res Technol 220, 312–315 (2005). https://doi.org/10.1007/s00217-004-1095-y
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DOI: https://doi.org/10.1007/s00217-004-1095-y