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Molecular identification of cephalopod species by FINS and PCR-RFLP of a cytochrome b gene fragment

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Abstract

Identification of commercial species is a relevant issue to assure the correct labeling of seafood products. In this work two different molecular techniques, FINS (Forensically Informative Nucleotide Sequencing) and PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) were developed to identify 8 cephalopod species (families Loliginidae and Ommastrephidae) employing a fragment of the cytochrome b gene. DNA amplification for all of the species was carried out with a new set of specific primers designed in this study for cephalopods. FINS is a technique based on DNA sequencing, while PCR-RFLP allows direct species identification by comparing specific DNA restriction patterns. Both techniques are useful for cephalopod species identification. 17 food products (mainly "squid rings") were analyzed and the species employed for their manufacture identified by FINS.

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References

  1. Roper CFE, Sweeney MJ, Nauen CE (1984) FAO Fish Synop 125:273

    Google Scholar 

  2. Shaw PW, Pierce GJ, Boyle PR (1999) Mol Ecol 8:407

    CAS  Google Scholar 

  3. Roper CFE, Mangold KM (1998) Systematics and distributional relationships of Illex coindetii to the genus Illex (Cephalopoda:Ommastrephidae). In: Rodhouse PG, Dawe EG, O'Dor RK (eds) Squid recruitment dynamics: The genus Illex as a model, the commercial Illex species, and influences on variability, vol. 376. Fisheries Technical Paper. FAO, Rome, p13

  4. Terio E, Tiecco G, Tantillo G (1983) Riv Zoot Vet 11:214

    Google Scholar 

  5. Sotelo CG, Piñeiro C, Gallardo JM, Pérez-Martín RI (1993) Trends Food Sci Tech 4:395

    Google Scholar 

  6. Shaw PW (1997) Mol Ecol 6:297

    CAS  Google Scholar 

  7. De Los Angeles Barriga Sosa I, Beckenbach K, Hartwick B, Smith MJ (1995) Mol Phyl Evol 4:163

    Article  Google Scholar 

  8. Söller R, Warnke K, Saint-Paul U, Blohm D (2000) Mar Biol 136:29

    Article  Google Scholar 

  9. Bonnaud L, Boucher-Rodoni R, Monnerot M (1994) C R Acad Sci Paris, Sciences de la vie/Life sciences 317:581

  10. Bonnaud L, Boucher-Rodoni R, Monnerot M (1997) Mol Phyl Evol 7:44

    Article  CAS  Google Scholar 

  11. Anderson FE (2000) Mol Phyl Evol 15:191

    Article  CAS  Google Scholar 

  12. Chapela MJ, Sotelo CG, Calo-Mata P, Pérez-Martín RI, Rehbein H, Hold GL, Quinteiro J, Rey-Méndez M, Rosa C, Santos AT (2002) J Food Sci 67:1672

    CAS  Google Scholar 

  13. Colombo F, Cerioli M, Colombo MM, Marchisio E, Malandra R, Renon P (2002) Food Control 13:185

    Article  CAS  Google Scholar 

  14. Quinteiro J, Sotelo CG, Rehbein H, Pryde SE, Medina I, Pérez-Martín RI, Rey-Méndez M, Mackie IM (1998) J Agric Food Chem 46:1662

    Article  CAS  Google Scholar 

  15. Carrera E, García T, Céspedes A, González I, Fernández A, Hernández PE, Martín R (1999) J Food Sci 64:410

    CAS  Google Scholar 

  16. Russel VJ, Hold GL, Pryde S, Rehbein H, Quinteiro J, Rey-Méndez M, Sotelo CG; Pérez-Martín RI, Santos AT, Rosa C (2000) J Agric Food Chem 48:2184

    Article  PubMed  Google Scholar 

  17. Cocolin L, D'Agaro E, Manzano M, Lanari D, Comi G (2000) J Food Sci 65:1315

    CAS  Google Scholar 

  18. Hold GL, Russel VJ, Pryde SE, Rehbein H, Quinteiro J, Vidal R, Rey-Méndez M, Sotelo CG, Pérez-Martín RI, Santos AT, Rosa C (2001) J Agric Food Chem 49:1175

    Article  CAS  PubMed  Google Scholar 

  19. Sotelo CG, Calo-Mata P, Chapela MJ, Pérez-Martín RI, Rehbein H, Hold GL, Russel VJ, Pryde S, Quinteiro J, Izquierdo M, Rey-Méndez M, Rosa C, Santos AT (2001) J Agric Food Chem 49:4562

    Article  CAS  PubMed  Google Scholar 

  20. Quinteiro J, Vidal R, Izquierdo M, Sotelo CG, Chapela MJ, Pérez-Martín RI, Rehbein H, Hold GH, Russel V, Pryde S, Rosa C, Santos AT, Rey-Méndez M (2001) J Agric Food Chem 49:5108

    Article  CAS  PubMed  Google Scholar 

  21. Rehbein H, Sotelo CG, Pérez-Martín RI, Chapela MJ, Hold GL, Russel VJ, Pryde SE, Santos AT, Rosa C, Quinteiro J, Rey-Méndez M (2002) Eur Food Res Technol 214:171

    Article  CAS  Google Scholar 

  22. Wolf C, Hübner P, Lüthy J (1999) Food Research International 32:699

    Article  CAS  Google Scholar 

  23. Borgo R, Souty-Grosset C, Bouchon D, Gomot L (1996) J Food Sci 61:1

    CAS  Google Scholar 

  24. Forrest ARR, Carnegie PR (1994) Biotech 17:24

    CAS  Google Scholar 

  25. Merrit TJS, Shi L, Chase MC, Rex MA, Etter RJ, Quattro JM (1998) Mol Mar Biol Biotech 7:7

    Google Scholar 

  26. Thompson JD, Higgins DG, Gibson TJ (1994) Nucl Acids Res 22:4673

    CAS  Google Scholar 

  27. Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA 2: Molecular evolutionary genetics analysis software. Arizona State University, Tempe, Az, USA

    Google Scholar 

  28. Heuskeshoven J, Dermick R (1985) Electrophoresis 6:103

    CAS  Google Scholar 

  29. Dieffenbach CW, Lowe TMJ, Dveksler GS (1993) PCR Meth App 3:S30

    CAS  Google Scholar 

  30. Bartlett SE, Davidson WS (1992) BioTech 12:408

    CAS  PubMed  Google Scholar 

  31. Palumbi SR (1996) Nucleic acids II: The polymerase chain reaction. In: Hillis DM, Moritz C, Mable BK (eds) Molecular Systematics. Sinauer Associates Inc, Sunderland, Massachusetts, p205

  32. Brodmann PD, Nicholas G, Schaltenbrand P, Ilg EC (2001) Eur Food Res Technol 212:491

    Article  CAS  Google Scholar 

  33. Céspedes A, García T, Carrera E, González I, Fernández A, Asensio L, Hernández PE, Martín R (2000) J Sci Food Agr 80:29

    Article  Google Scholar 

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Acknowledgements

The authors are grateful to the European Commission, Xunta de Galicia and Comisión Interministerial de Ciencia y Tecnología for their financial support through research grants FAIR CT97–3061, XUGA 40202 B 98 and ALI1997–1972-CE.

Author Chapela thanks Instituto Danone for awarding her a doctoral fellowship.

We thank Paul Beck and Earl Dawe from the Northwest Atlantic Fisheries Center (Canada) for sending us samples of Illex illecebrosus and Julio Maroto from Pez Austral (Spain) who provided samples of Martialia hyadesi and Illex argentinus. We also thank Susana Otero, Marta Pérez and Helena Pazó for technical assistance.

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  1. Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas, Eduardo Cabello 6, 36208 Pontevedra, Vigo, Spain

    María José Chapela, Carmen G. Sotelo & Ricardo I. Pérez-Martín

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  1. María José Chapela
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  2. Carmen G. Sotelo
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  3. Ricardo I. Pérez-Martín
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Correspondence to María José Chapela.

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Chapela, M.J., Sotelo, C.G. & Pérez-Martín, R.I. Molecular identification of cephalopod species by FINS and PCR-RFLP of a cytochrome b gene fragment. Eur Food Res Technol 217, 524–529 (2003). https://doi.org/10.1007/s00217-003-0788-y

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  • DOI: https://doi.org/10.1007/s00217-003-0788-y

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