Abstract
A method for the determination of cyanide in blood plasma by differential pulse polarography (DPP) is described without a drastic acidification of the sample. Cyanide was determined as tetracyanonickelate(II)-anion complex after a microwave-acid assisted cleanup and a selective complex extraction in a polyethylene methylene blue (PE-MB) impregnated column. The cyano complex was eluted from the column with water/acetonitrile and determined by pulse-polarography at –380 mV (Ag/AgCl). The linear range of calibration was obtained from 1.2 to 9.6 μg of cyanide with r = 0.99 and RSD = 9% of 1.2 μg of cyanide. A detection limit of 40 μg L–1 was calculated and the recoveries of cyanide from spiked samples were about 80%. This method was compared with the classical pyridine-pyrazolone method.
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Received: 3 September 1997 / Revised: 21 January 1998 / Accepted: 24 January 1998
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Bohrer, D., do Nascimento, P., Garcia Pomblum, S. et al. Polarographic determination of cyanide as nickelcyano complex in blood plasma after selective extraction in a methylene blue impregnated polyethylene column. Fresenius J Anal Chem 361, 780–783 (1998). https://doi.org/10.1007/s002160051015
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DOI: https://doi.org/10.1007/s002160051015