Abstract
A novel H2O2 biosensor was constructed employing α-zirconium phosphate as a new support substrate to hold an electron shuttle toluidine blue between a glassy carbon electrode and horseradish peroxidase. Toluidine blue was intercalated into α-zirconium phosphate-modified horseradish peroxidase immobilization matrix cross-linked on a glassy carbon electrode surface via bovine serum albumin-glutaraldehyde. This co-immobilization matrix of the mediator and the enzyme was formed from the α-zirconium phosphate (α-ZrP)-toluidine blue (TB) inclusion colloid in which horseradish peroxidase (HRP) was dissolved. Intercalation of TB in layered α-ZrP was investigated by scanning electron microscopy (SEM), X-ray powder diffraction (XRD) and electrochemical measurements. TB immobilized in this way underwent a quasi-reversible electrochemical redox reaction at the electrode. Cyclic voltammetry and amperometric measurements demonstrated good stability and efficiently-shuttled electrons between HRP and the electrode. The sensor responded rapidly to H2O2 with a detection limit of 3.0 × 10–7 mol/L.
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Received: 1 July 1997 / Revised: 13 October 1997 / Accepted: 21 October 1997
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Yang, F., Ruan, C., Xu, J. et al. An amperometric biosensor using toluidine blue as an electron transfer mediator intercalated in α-zirconium phosphate-modified horseradish peroxidase immobilization matrix. Fresenius J Anal Chem 361, 115–118 (1998). https://doi.org/10.1007/s002160050845
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DOI: https://doi.org/10.1007/s002160050845