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Analysis of phytochelatins in plant matrices by pre-column derivatization, high-performance liquid chromatography and fluorescence-detection

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Abstract

A sensitive method for the determination of phytochelatins in plant matrices by pre-column derivatization with monobromobimane (mBrB) and high-performance liquid chromatography (HPLC) on reversed phases and fluorescence-detection has been developed and applied to cucumber sprouts (Cucumis sativus) treated with cadmium and to the water moss Fontinalis antipyretica (Cd in environmentally-relevant concentrations). Whereas phytochelatins were found in the Cd-treated sprouts, no phytochelatins were detected in Fontinalis antipyretica.

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Authors and Affiliations

  1. Internationales Hochschulinstitut Zittau, Lehrstuhl Umweltverfahrenstechnik, Markt 23, 02763 Zittau, Germany e-mail: korhammer@ihi-zittau.de, Germany

    S. Döring, S. Korhammer, M. Oetken & B. Markert

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  1. S. Döring
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  2. S. Korhammer
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  3. M. Oetken
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  4. B. Markert
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Received: 22 July 1999 / Revised: 20 September 1999 / /Accepted: 27 September 1999

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Döring, S., Korhammer, S., Oetken, M. et al. Analysis of phytochelatins in plant matrices by pre-column derivatization, high-performance liquid chromatography and fluorescence-detection. Fresenius J Anal Chem 366, 316–318 (2000). https://doi.org/10.1007/s002160050062

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  • DOI: https://doi.org/10.1007/s002160050062

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