Abstract
A simple, reliable and reproducible method, based on capillary zone electrophoresis with amperometric detection, has been developed for the determination of idarubicin in human urine. A carbon disk electrode was used as working electrode. The optimal conditions of separation and detection were pH 5.6 phosphate buffer ¶(0.20 mol/L), 22 kV for the separation voltage and 1.00 V (vs. Ag/AgCl, 3 mol/L KCl) for the detection potential. The linear range was from 4.0 × 10–7 to 2.0 × 10–5 mol/L with a regression coefficient of 0.9986, and the detection limit was 8.0 × 10–8 mol/L. The method was directly applied to the determination of idarubicin in spiked human urine without any other sample pretreatment except filtration, and the assay results were satisfactory.
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Received: 23 May 2000 / Revised: 8 August 2000 / Accepted: 9 August 2000
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Hu, Q., Zhou, T., Zhang, L. et al. Determination of idarubicin in human urine by capillary zone electrophoresis with amperometric detection. Fresenius J Anal Chem 368, 844–847 (2000). https://doi.org/10.1007/s002160000571
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DOI: https://doi.org/10.1007/s002160000571