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A dual-signal fluorescent probe for detection of acid phosphatase

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Abstract

Acid phosphatase has become a significant indicator of prognostic and medical diagnosis, and its dysfunction may lead to a series of diseases. A novel dual-signal fluorescence method for acid phosphatase detection based on europium polymer (europium-pyridine dicarboxylicacid-adenine) and pyridoxal phosphate (PLP) was proposed. PLP coordinated with europium polymer via Eu3+ and P–O bonds, and the fluorescence of europium polymer was quenched due to the photoinduced electron transfer (PET) effect between aldehyde and europium polymer. Upon addition of acid phosphatase, the PLP was transformed to phosphate (Pi) and pyridoxal (PL). The PL was released from the surface of europium polymer, and the blue emission was enhanced due to the formation of internal hemiacetal, while the fluorescence of europium polymer recovered. The blue (PL) and red emission (Eu3+) were positively correlated with acid phosphatase activity; thus the sensitive assay of acid phosphatase was effectively achieved. The two signals were applied to determine the acid phosphatase with limits of detection (LOD) of 0.04 mU/mL and 0.38 mU/mL, and the linear ranges were 0.13–5.00 mU/mL and 1.25–20.00 mU/mL, respectively. The probe can be used to trace the acid phosphatase in biological systems and holds promise for use in clinical diagnosis and early prevention.

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Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (No. 21575055), the Henan Key Laboratory of Biomolecular Recognition and Sensing (HKLBRSK1901) and the Fundamental Research Funds for the Central Universities (No. 561220006).

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Authors and Affiliations

  1. State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, Gansu, China

    Shuangqin Li, Guoqing Fu, Yaya Wang, Yueci Xiang, Shuai Mu, Yixuan Xu, Xiaoyan Liu & Haixia Zhang

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  1. Shuangqin Li
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  2. Guoqing Fu
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  3. Yaya Wang
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  4. Yueci Xiang
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  7. Xiaoyan Liu
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  8. Haixia Zhang
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Contributions

Shuangqin Li: Scheme design, Probe synthesis, Methodology, Validation, Formal analysis, Writing—original draft. Guoqing Fu: Schematic diagram drawing. Yaya Wang: Formal analysis. Yueci Xiang: Formal analysis. Shuai Mu: Formal analysis. Yixuan Xu: Discussion of experimental mechanism. Xiaoyan Liu: Essay review and grammar revision. Haixia Zhang: Writing—review & editing, Supervision.

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Correspondence to Haixia Zhang.

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The fresh serum samples were acquired from healthy individuals in the infirmary of Lanzhou University in accordance with the rules of the local ethical committee.

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Li, S., Fu, G., Wang, Y. et al. A dual-signal fluorescent probe for detection of acid phosphatase. Anal Bioanal Chem 413, 3925–3932 (2021). https://doi.org/10.1007/s00216-021-03343-2

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  • DOI: https://doi.org/10.1007/s00216-021-03343-2

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