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Au-coated Fe3O4 core-shell nanohybrids with photothermal activity for point-of-care immunoassay for lipoprotein-associated phospholipase A2 on a digital near-infrared thermometer

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Abstract

A portable photothermal immunoassay based on Au-coated magnetic Fe3O4 core-shell nanohybrids (Au-Fe3O4) was developed for point-of-care (POC) testing of lipoprotein-associated phospholipase A2 (Lp-PLA2) on a digital near-infrared (NIR) thermometer. Au-Fe3O4 photothermal materials were first synthesized through reverse micelle method, and then functionalized with polyclonal rabbit anti-human Lp-PLA2 antibody. A sandwiched immunoreaction was carried out in polyclonal mouse anti-human Lp-PLA2 antibody-coated microplate using Au-Fe3O4-labeled antibody as the detection antibody. With formation of sandwich-type immunocomplex, the captured Au-Fe3O4 on the plate converted the light into heat under an 808-nm laser irradiation (1.5 W cm−2), thereby resulting in the increasing temperature of the detection solution. The temperature variations relative to surrounding temperature was determined on a portable NIR thermometer. Several labeling protocols with gold nanoparticle, Fe3O4 nanoparticle, or Au-Fe3O4 nanohybrids were investigated for determination of Lp-PLA2 and improved analytical features were achieved with the core-shell Au-Fe3O4 nanohybrids. Under optimum conditions, Au-Fe3O4-based immunoassay exhibited good photothermal responses for the detection of Lp-PLA2 with a dynamic linear range of 0.01–100 ng mL−1 at a low detection limit of 8.6 pg mL−1. Good reproducibility and intermediate precision were less than 9.7%. Other biomarkers or proteins did not interfere with responses of this system. An acceptable accuracy was acquired for analysis of human serum sample between Au-Fe3O4-based photothermal immunoassay and commercialized human Lp-PLA2 ELISA kit.

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Funding

Support by the Training Project of Young Talents in Health System of Fujian Province, China (grant no.: 2019-ZQNB-9) is gratefully acknowledged.

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Author notes

  1. Haixin Guo and Xiaoping Su contributed equally to this work.

Authors and Affiliations

  1. Department of Ultrasound, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian, China

    Haixin Guo

  2. Department of Urology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian, China

    Xiaoping Su, Qingfu Su & Wei Zhuang

  3. Department of Urology, Jinjiang Municipal Hospital, No. 392, Xinhua Street, Jinjiang City, 362200, Fujian, China

    Zhijiao You

Authors
  1. Haixin Guo
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  2. Xiaoping Su
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  3. Qingfu Su
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  4. Wei Zhuang
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  5. Zhijiao You
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Corresponding author

Correspondence to Zhijiao You.

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All procedures performed in studied involving human participants were approved by Fujian Medical University, and in accordance with the ethical standards of “Fujian Medical University” and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Guo, H., Su, X., Su, Q. et al. Au-coated Fe3O4 core-shell nanohybrids with photothermal activity for point-of-care immunoassay for lipoprotein-associated phospholipase A2 on a digital near-infrared thermometer. Anal Bioanal Chem 413, 235–244 (2021). https://doi.org/10.1007/s00216-020-02995-w

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  • DOI: https://doi.org/10.1007/s00216-020-02995-w

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