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A visual electrochemiluminescence biosensor based on CuInZnS quantum dots for superoxide dismutase detection

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Abstract

Superoxide dismutase (SOD), also known as liver protein, is a substance widely distributed in various biological cells. It has the function of catalyzing the disproportionation reaction of superoxide free radicals. SOD can form an antioxidant chain together with peroxidase, catalase, and other substances in the body of organisms, and thus, is one of the indispensable important substances in the body of organisms. In this work, we provided a simple and fast visual electrochemiluminescence (ECL) sensor for SOD detection. CuInZnS quantum dots (QDs) worked as the ECL luminophore with hydrogen peroxide as co-reactant. In the sensing process, SOD and CuInZnS QDs on a glassy carbon electrode (GCE) competed with each other for hydrogen peroxide to produce superoxide during electrochemical luminescence, thus quenching the ECL signal of CuInZnS QDs. The proposed sensor can quantify SOD with a limit of detection (LOD) of 0.03 μg/mL. In addition, the change in the CuInZnS QDs ECL signal was easily observed with a smartphone camera. The results indicated that this sensor could effectively work in the detection of SOD in human blood.

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Funding

This study received financial support from the National Natural Science Foundation of China (No 21005029), Youth Science Fund of Jilin Province (No 20140520081JH), and “Thirteenth Five Year” Project of the Science and Technology Research in the Education Department of Jilin Province, China (No JJKH20180125KJ).

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Authors and Affiliations

  1. Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, Jilin, China

    Yang Liu, Kunliang Jiang, Yixin Nie, Yupeng Guo & Qiang Ma

  2. National Chemistry Experimental Teaching Demonstration Center, Jilin University, Changchun, 130012, Jilin, China

    Yang Liu, Kunliang Jiang, Yixin Nie, Yupeng Guo & Qiang Ma

Authors
  1. Yang Liu
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  2. Kunliang Jiang
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  3. Yixin Nie
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  4. Yupeng Guo
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  5. Qiang Ma
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Corresponding author

Correspondence to Qiang Ma.

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Statement on ethical approval

The use of human serum samples in this study has been approved by the ethics committee of the China-Japan Union Hospital of Jilin University (No. 2019-NSFC-051).

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The authors declare that there are no conflicts of interest.

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Liu, Y., Jiang, K., Nie, Y. et al. A visual electrochemiluminescence biosensor based on CuInZnS quantum dots for superoxide dismutase detection. Anal Bioanal Chem 412, 1893–1899 (2020). https://doi.org/10.1007/s00216-020-02440-y

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  • DOI: https://doi.org/10.1007/s00216-020-02440-y

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