Abstract
The significance of wood odors beyond flavoring effects for barbecues and wine aged in barrels has recently been under discussion. Wood has an immense influence on our physical and mental condition through our palate, such as positive health and mood-stimulating effects. As a result of increased public interest, the key odorants from various natural wood species were recently decoded. To gain profound insights into the contribution of single odorants to the overall scent of distinct wood types, this study compares several softwood species (Scots pine, incense cedar, European larch, Norway spruce, and white fir) by odor profile analysis. Nine odor-active constituents, which were previously detected in those woods, were quantified using stable isotope dilution analysis (SIDA). Odor activity values (OAVs) calculated on the basis of odor thresholds (OTs) determined in cellulose revealed hexanal, octanal, (E)-non-2-enal, p-cresol, vanillin, and thymoquinone as dominant odorants for wood odor. Recombination experiments by mixing the odorants in their naturally occurring concentrations in a cellulose matrix confirmed the successful characterization of the key odorants for Scots pine and incense cedar wood.
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References
Rametsteiner E, Oberwimmer R, Gschwandtl I, editors. Europeans and wood. What do Europeans think about wood and its uses? A review of consumer and business surveys in Europe: Ministerial Conference on the Protection of Forests in Europe Liaison Unit Warsaw. 2007.
Kardan O, Gozdyra P, Misic B, Moola F, Palmer LJ, Paus T, et al. Neighborhood greenspace and health in a large urban center. Sci Rep. 2015;5:11610.
Hunter MR. Urban nature experiences reduce stress in the context of daily life based on salivary biomarkers. Front Psychol. 2019;10:722.
Hansen MM, Jones R, Tocchini K. Shinrin-yoku (forest bathing) and nature therapy: a state-of-the-art review. Int J Environ Res Public Health. 2017;14(8):851.
Ross RJ. Wood handbook: wood as an engineering material, vol. 1. Madison, WI: USDA Forest Service, Forest Products Laboratory, General Technical Report FPL-GTR-190; 2010. p. 509. 190
Fengel D, Wegener G. Wood: chemistry, ultrastructure, reactions. Berlin: De Gruyter; 1983.
Miller RB. Characteristics and availability of commercially important woods. 1999;113.
Culleré L, Fernández de Simón B, Cadahía E, Ferreira V, Hernández-Orte P, Cacho J. Characterization by gas chromatography–olfactometry of the most odor-active compounds in extracts prepared from acacia, chestnut, cherry, ash and oak woods. LWT Food Sci Technol. 2013;53(1):240–8.
Díaz-Maroto MC, Guchu E, Castro-Vázquez L, de Torres C, Pérez-Coello MS. Aroma-active compounds of American, French, Hungarian and Russian oak woods, studied by GC–MS and GC–O. Flavour Frag. J. 2008;23(2):93–8.
Ghadiriasli R, Wagenstaller M, Buettner A. Identification of odorous compounds in oak wood using odor extract dilution analysis and two-dimensional gas chromatography-mass spectrometry/olfactometry. Anal Bioanal Chem. 2018;410(25): 6595-6607.
Schreiner L, Bauer P, Buettner A. Resolving the smell of wood—identification of odour-active compounds in Scots pine (Pinus sylvestris L.). Sci Rep. 2018;8(1):8294.
Schreiner L, Loos HM, Buettner A. Identification of odorants in wood of Calocedrus decurrens (Torr.) Florin by aroma extract dilution analysis and two-dimensional gas chromatography–mass spectrometry/olfactometry. Anal Bioanal Chem. 2017;409(15):3719–29.
Wagenführ R. Holzatlas. Leipzig: Fachbuchverlag; 2007.
Buksnowitz C, Teischinger A, Müller U, Pahler A, Evans R. Resonance wood [Picea abies (L.) Karst.]—evaluation and prediction of violin makers’ quality-grading. J Acoust Soc Am. 2007;121(4):2384–95.
Roloff A. Bäume Nordamerikas: Von Alligator-Wachholder bis Zuckerahorn. Alle charakteristischen Arten im Porträt. Weinheim: Wiley-VCH; 2010. p. 501–3.
Laacke RJ. Abies concolor (Gord. & Glend.) Lindl. ex Hildebr. White fir. Silvics of North America. 1990;1:36–46.
Veluthoor S, Kelsey RG, González-Hernández M, Panella N, Dolan M, Karchesy J. Composition of the heartwood essential oil of incense cedar (Calocedrus decurrens Torr.) Holzforschung. 2011;65(3):333–6.
Kwak CS, Moon SC, Lee MS. Antioxidant, antimutagenic, and antitumor effects of pine needles (Pinus densiflora). Nutr Cancer. 2006;56(2):162–71.
Judžentienė A, Šližytė J, Stiklienė A, Kupčinskienė E. Characteristics of essential oil composition in the needles of young stand of Scots pine ( Pinus sylvestris L.) growing along aerial ammonia gradient. Chemija. 2006;17(2):67–73.
Bumgardner MS, Bowe SA. Species selection in secondary wood products: implications for product design and promotion. Wood Fiber Sci. 2007;34(3):408–18.
Iqbal N, Mustafa G, Rehman A, Biedermann A, Najafi B, Lieberzeit PA, et al. QCM-arrays for sensing terpenes in fresh and dried herbs via bio-mimetic MIP layers. Sensors. 2010;10(7):6361–76.
Johnson-Ajinwo OR, Li W-W. Stable isotope dilution gas chromatography–mass spectrometry for quantification of thymoquinone in black cumin seed oil. J Agric Food Chem. 2014;62(24):5466–71.
Frank O, Kreissl JK, Daschner A, Hofmann T. Accurate determination of reference materials and natural isolates by means of quantitative 1H NMR spectroscopy. J Agric Food Chem. 2014;62(12):2506–15.
Czerny M, Christlbauer M, Christlbauer M, Fischer A, Granvogl M, Hammer M, et al. Re-investigation on odour thresholds of key food aroma compounds and development of an aroma language based on odour qualities of defined aqueous odorant solutions. Eur Food Res Technol. 2008;228(2):265–73.
Lawless HT, Heymann H. Sensory evaluation of food: principles and practices. Nwe York: Springer; 1999.
Schieberle P, Grosch W. Quantitative analysis of aroma compounds in wheat and rye bread crusts using a stable isotope dilution assay. J Agric Food Chem. 1987;35(2):252–7.
Engel W, Bahr W, Schieberle P. Solvent assisted flavour evaporation—a new and versatile technique for the careful and direct isolation of aroma compounds from complex food matrices. Eur Food Res Technol. 1999;209(3):237–41.
Bemelmans J. Review of isolation and concentration techniques. Prog Flavour Res. 1979;8:79–98.
Candelier K, Dumarçay S, Pétrissans A, Pétrissans M, Kamdem P, Gérardin P. Thermodesorption coupled to GC–MS to characterize volatiles formation kinetic during wood thermodegradation. J Anal Appl Pyrolysis. 2013;101:96–102.
Pińkowska H, Wolak P, Złocińska A. Hydrothermal decomposition of alkali lignin in sub-and supercritical water. Chem Eng J. 2012;187:410–4.
Hillis W, Inoue T. The formation of polyphenols in trees—IV: the polyphenols formed in Pinus radiata after Sirex attack. Phytochemistry. 1968;7(1):13–22.
Grosch W. Evaluation of the key odorants of foods by dilution experiments, aroma models and omission. Chem Senses. 2001;26(5):533–45.
Czerny M, Grosch W. Potent odorants of raw Arabica coffee. Their changes during roasting. J Agric Food Chem. 2000;48(3):868–72.
Czerny M, Buettner A. Odor-active compounds in cardboard. J Agric Food Chem. 2009;57(21):9979–84.
Siefarth C, Serfert Y, Drusch S, Buettner A. Comparative evaluation of diagnostic tools for oxidative deterioration of polyunsaturated fatty acid-enriched infant formulas during storage. Foods. 2014;3(1):30–65.
Kollmann F. Anatomie und Pathologie, Chemie, Physik Elastizität und Festigkeit: 1. Band. Berlin: Springer; 2013.
Tsoumis G. Wood as raw material: source, structure, chemical composition, growth, degradation and identification. Amsterdam: Elsevier Science; 2013.
Risholm-Sundman M, Lundgren M, Vestin E, Herder P. Emissions of acetic acid and other volatile organic compounds from different species of solid wood. Holz Roh Werkst. 1998;56(2):125–9.
Baumann MG, Batterman SA, Zhang G-Z. Terpene emissions from particleboard and medium-density fiberboard products. For Prod J. 1999;49(1):49–56.
Sjöström E. Wood chemistry: fundamentals and applications. New York: Academic Press; 1981.
Geladi P, Arshadi M, Gref R, Fjällström P. Emission of volatile aldehydes and ketones from wood pellets under controlled conditions. Ann Occup Hyg. 2009;53(8):797–805.
Alves A, Gierlinger N, Schwanninger M, Rodrigues J. Analytical pyrolysis as a direct method to determine the lignin content in wood: part 3. Evaluation of species-specific and tissue-specific differences in softwood lignin composition using principal component analysis. J Anal Appl Pyrolysis. 2009;85(1–2):30–7.
Zavarin E, Anderson AB. Extractive components from incense-cedar heartwood (Libocedrus decurrens Torrey) I. Occurrence of carvacrol, hydrothymoquinone, and thymoquinone. J. Org. Chem. 1955;20(1):82–8.
Scheffer TC. Natural resistance of wood to microbial deterioration. Annu Rev Phytopathol. 1966;4(1):147–68.
Overend RP, Milne T, Mudge L. Fundamentals of thermochemical biomass conversion. Netherlands: Springer; 2012.
Komenda M, Koppmann R. Monoterpene emissions from Scots pine ( Pinus sylvestris): field studies of emission rate variabilities. J Geophys Res Atmos. 2002;107(D13), ACH-1.
Silvério FO, Barbosa LC, Maltha CR, Fidêncio PH, Cruz MP, Veloso DP, et al. Effect of storage time on the composition and content of wood extractives in Eucalyptus cultivated in Brazil. Bioresour Technol. 2008;99(11):4878–86.
Morais MC, Pereira H. Variation of extractives content in heartwood and sapwood of Eucalyptus globulus trees. Wood Sci Technol. 2012;46(4):709–19.
Plotto A, Margaría CA, Goodner KL, Goodrich R, Baldwin EA. Odour and flavour thresholds for key aroma components in an orange juice matrix: terpenes and aldehydes. Flavour Frag. J. 2004;19(6):491-8.
Buettner A, Schieberle P. Evaluation of aroma differences between hand-squeezed juices from Valencia late and navel oranges by quantitation of key odorants and flavor reconstitution experiments. J Agric Food Chem. 2001;49(5):2387–94.
Acknowledgments
We are grateful to Dr. Alexander Vyhnal and Staedtler Mars GmbH & Co KG for supplying the incense cedar, white fir, and Scots pine wood samples as well as to Richard Hammerl for providing help regarding NMR analyses and to the members of our working group for their participation in the sensory analyses. This study was carried out within the framework of the Campus of the Senses, a joint endeavor of the Fraunhofer Institutes for Process Engineering and Packaging IVV and Integrated Circuits IIS, together with Friedrich-Alexander-Universität Erlangen-Nürnberg as an academic partner. The Campus of the Senses initiative is financially supported by the Bavarian Ministry of Economic Affairs, Regional Development and Energy (StMWi), and the Fraunhofer society.
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The study was conducted in agreement with the Declaration of Helsinki. The study (registration number 180_16B) was approved by the Ethical Committee of the Medical Faculty, Friedrich-Alexander Universität Erlangen-Nürnberg. Informed consent was obtained from all subjects participating in the study.
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Schreiner, L., Ortner, E. & Buettner, A. Nosy confirmation: reconstitution of the characteristic odor of softwood via quantitative analysis and human sensory evaluation. Anal Bioanal Chem 412, 1137–1149 (2020). https://doi.org/10.1007/s00216-019-02339-3
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DOI: https://doi.org/10.1007/s00216-019-02339-3