Skip to main content
SpringerLink
Log in

Oriented assembly of surface plasmon resonance biosensor through staphylococcal protein A for the chlorpyrifos detection

  • Research Paper
  • Published:
Analytical and Bioanalytical Chemistry Aims and scope Submit manuscript

Abstract

In this study, we report a direct surface plasmon resonance (SPR) biosensor based on an oriented assembly of antibody for the rapid detection of chlorpyrifos residue in agricultural samples. In this covalent-orientated strategy, staphylococcal protein A (SPA) was first covalently bound to the surface for monitoring chlorpyrifos residue, with subsequent binding of the antibody in an orientated fashion via its fragment crystallizable (Fc) region. Consequently, the SPA-modified biosensor exhibited a satisfactory specificity and a low detection limit of 0.056 ng mL−1 for chlorpyrifos, with a linear detection range of 0.25–50.0 ng mL−1. Under optimal conditions, the sensor chip could be regenerated for at least 210 cycles. The results presented here indicate that the SPA-modified sensor chip can successfully improve the sensitivity and obviating the need of the modification of the antibody. The developed SPR biosensor method has the great potential for rapid, sensitive, and specific detection with broad applications in areas of environmental monitoring and food safety.

Graphical abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Harshit D, Charmy K, Nrupesh P. Organophosphorus pesticides determination by novel HPLC and spectrophotometric method. Food Chem. 2017;230:448–53.

    Article  CAS  PubMed  Google Scholar 

  2. Gupta SC, Mishra M, Sharma A, Deepak Balaji TG, Kumar R, Mishra RK, et al. Chlorpyrifos induces apoptosis and DNA damage in Drosophila through generation of reactive oxygen species. Ecotoxicol Environ Saf. 2010;73(6):1415–23.

    Article  CAS  PubMed  Google Scholar 

  3. Sotomayor V, Lascano C, D'Angelo AMP, Venturino A. Developmental and polyamine metabolism alterations in Rhinella arenarum embryos exposed to the organophosphate chlorpyrifos. Environ Toxicol Chem. 2012;31:2052–8.

    Article  CAS  PubMed  Google Scholar 

  4. Khalil AM. Toxicological effects and oxidative stress responses in freshwater snail, Lanistes carinatus, following exposure to chlorpyrifos. Ecotoxicol Environ Saf. 2015;116:137–42.

    Article  CAS  PubMed  Google Scholar 

  5. Lee W, Byung Keun O, Min Bae Y, Paek SH, Hong Lee W, Choi JW. Fabrication of self-assembled protein a monolayer and its application as an immunosensor. Biosens Bioelectron. 2003;19(3):185–92.

    Article  CAS  PubMed  Google Scholar 

  6. Dou XW, Zhang L, Liu C, Li Q, Luo J, Yang MH. Fluorometric competitive immunoassay for chlorpyrifos using rhodamine-modified gold nanoparticles as a label. Microchimca Acta. 2017;185(1):41.

  7. Kim Young AH, Lee EH, Kim Kwang OK, Lee YT, Hammock BD, Lee HS. Competitive immunochromatographic assay for the detection of the organophosphorus pesticide chlorpyrifos. Anal Chim Acta. 2011;693:106–13.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Xu ZL, Wang Q, Lei HT, Eremin SA, Shen YD, Wang H, et al. A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples. Anal Chim Acta. 2011;708(1–2):123–9.

    Article  CAS  PubMed  Google Scholar 

  9. Sun Z, Wang W, Wen H, Gan C, Lei H, Liu Y. Sensitive electrochemical immunoassay for chlorpyrifos by using flake-like Fe3O4 modified carbon nanotubes as the enhanced multienzyme label. Anal Chim Acta. 2015;899:91–9.

    Article  CAS  PubMed  Google Scholar 

  10. Daems D, Lu J, Delport F, Marien N, Orbie L, Aernouts B, et al. Competitive inhibition assay for the detection of progesterone in dairy milk using a fiber optic SPR biosensor. Anal Chim Acta. 2017;950:1–6.

    Article  CAS  PubMed  Google Scholar 

  11. Shi S, Wang L, Su R, Liu B, Huang R, Qi W, et al. A polydopamine-modified optical fiber SPR biosensor using electroless-plated gold films for immunoassays. Biosens Bioelectron. 2015;74:454–60.

    Article  CAS  PubMed  Google Scholar 

  12. Zhang P, Chen Y-P, Wang W, Shen Y, Guo J-S. Surface plasmon resonance for water pollutant detection and water process analysis. TrAC Trends Anal Chem. 2016;85:153–65.

    Article  CAS  Google Scholar 

  13. Gao YM, Zou F, Wu BP, Wang XX, Zhang JJ, Koh K, et al. CB[7]-mediated signal amplification approach for sensitive surface plasmon resonance spectroscopy. Biosens Bioelectron. 2016;81:207–13.

    Article  CAS  PubMed  Google Scholar 

  14. Fen YW, Yunus WM, Talib ZA, Yusof NA. Development of surface plasmon resonance sensor for determining zinc ion using novel active nanolayers as probe. Spectrochim Acta A. 2015;134:48–52.

    Article  CAS  Google Scholar 

  15. Masson JF. Surface plasmon resonance clinical biosensors for medical diagnostics. ACS Sens. 2017;2(1):16–30.

  16. Genslein C, Hausler P, Kirchner EM, Bierl R, Baeumner AJ, Hirsch T. Graphene-enhanced plasmonic nanohole arrays for environmental sensing in aqueous samples. Beilstein J Nanotech. 2016;7:1564–73.

  17. Vaisocherova-Lisalova H, Visova I, Ermini ML, Springer T, Song XC, Mrazek J, et al. Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples. Biosens Bioelectron. 2016;80:84–90.

    Article  CAS  PubMed  Google Scholar 

  18. Liu X, Hu Y, Zheng S, Liu Y, He Z, Luo F. Surface plasmon resonance immunosensor for fast, highly sensitive, and in situ detection of the magnetic nanoparticles-enriched Salmonella enteritidis. Sensors Actuators B Chem. 2016;230:191–8.

    Article  CAS  Google Scholar 

  19. Shankaran DR, Gobi KV, Miura N. Recent advancements in surface plasmon resonance immunosensors for detection of small molecules of biomedical, food and environmental interest. Sensors Actuators B Chem. 2007;121(1):158–77.

    Article  CAS  Google Scholar 

  20. Feng L, Zhu A, Wang H, Shi H. A nanosensor based on quantum-dot haptens for rapid, on-site immunoassay of cyanotoxin in environmental water. Biosens Bioelectron. 2014;53:1–4.

    Article  CAS  PubMed  Google Scholar 

  21. Li S, Wu Q, Ma P, Zhang Y, Song D, Wang X, et al. A sensitive SPR biosensor based on hollow gold nanospheres and improved sandwich assay with PDA-Ag-Fe3O4-rGO. Talanta. 2018;180:156–61.

    Article  CAS  PubMed  Google Scholar 

  22. Shynkarenko OV, Kravchenko SA. Surface plasmon resonance sensors: methods of surface functionalization and sensitivity enhancement. Theor Exp Chem. 2015;51(5):273–92.

    Article  CAS  Google Scholar 

  23. Vashist SK, Dixit CK, MacCraith BD, O’Kennedy R. Effect of antibody immobilization strategies on the analytical performance of a surface plasmon resonance-based immunoassay. Analyst. 2011;136(21):4431–6.

    Article  CAS  PubMed  Google Scholar 

  24. Lu B, Smyth MR, O'Kennedy R. Oriented immobilization of antibodies and its applications in immunoassays and immunosensors. Analyst. 1996;121:29–32.

    Article  Google Scholar 

  25. Mauriz E, Calle A, Lechuga LM, Quintana J, Montoya A, Manclús JJ. Real-time detection of chlorpyrifos at part per trillion levels in ground, surface and drinking water samples by a portable surface plasmon resonance immunosensor. Anal Chim Acta. 2006;561(1–2):40–7.

    Article  CAS  Google Scholar 

  26. Thepudom T, Lertvachirapaiboon C, Shinbo K, Kato K, Kaneko F, Kerdcharoen T, Baba A. Surface plasmon resonance-enhanced photoelectrochemical sensor for detection of an organophosphate pesticide chlorpyrifos. MRS Commun. 2017;8(01):107–12.

Download references

Acknowledgments

The authors acknowledge Beijing Inter-bio Tech CO., LTD, for providing the surface plasmon resonance device.

Funding

This work was supported by the National Natural Science Foundation of China (No. 81703699, 81573595, and 81603398), the National Project for Standardization of Chinese Materia Medica (No. ZYBZH-Y-JIN-34), CAMS Innovation Fund for Medical Sciences (No. 2017-I2M-1-013), and the Graduate Student Innovation Fund of Peking Union Medical College (No. 2017-1007-14).

Author information

Authors and Affiliations

  1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, 151 Malianwa North Road, Beijing, 100193, China

    Qian Li, Xiaowen Dou, Lei Zhang, Jiaoyang Luo & Meihua Yang

  2. Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Yaogu 4 Road, Xiuying District, Haikou, 570311, Hainan, China

    Xiangsheng Zhao & Meihua Yang

Authors
  1. Qian Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaowen Dou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lei Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiangsheng Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jiaoyang Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Meihua Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Meihua Yang.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, Q., Dou, X., Zhang, L. et al. Oriented assembly of surface plasmon resonance biosensor through staphylococcal protein A for the chlorpyrifos detection. Anal Bioanal Chem 411, 6057–6066 (2019). https://doi.org/10.1007/s00216-019-01990-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00216-019-01990-0

Keywords

Search

Navigation