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Binding properties of broad-specific monoclonal antibodies against three organophosphorus pesticides by a direct surface plasmon resonance immunosensor

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Abstract

In this study, heterologous indirect competitive enzyme-linked immunosorbent assay (icELISA) was introduced into the screening of hybridomas for the development of broad-specific monoclonal antibodies (mAbs) against organophosphorus (OP) pesticides. After immunization, two formats of icELISA based on the homologous hapten antigen and four heterologous hapten antigens were conducted for hybridoma screening. Two mAbs 2G6 and 7B2 with good recognition toward three OP pesticides (parathion, methyl-parathion, and fenitrothion) were produced. Results of the icELISA showed that the two mAbs exhibited high sensitivity against three OP pesticides, with IC50 ranging from 2.93 to 19.71 ng mL−1. Moreover, a non-competitive surface plasmon resonance (SPR) immunosensor was used for characterizing the binding properties of the mAbs to OP pesticides. After kinetic analysis, equilibrium dissociation constant (KD) values of mAbs 2G6 and 7B2 were calculated as 1.45 × 10−9 M and 4.26 × 10−9 M for parathion, 6.75 × 10−9 M and 4.17 × 10−9 M for methyl-parathion, and 2.44 × 10−8 M and 1.19 × 10−8 M for fenitrothion, respectively. Whereas, both icELISA and SPR-based immunoassay indicated that the two mAbs could not recognize other five OP analogs. Since SPR-based immunoassay provides comprehensive information of two molecules directly interacting with each other, it is a valuable tool during the development and characterization of broad-specific mAbs.

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Acknowledgements

This study was financially supported by National Key R&D Program of China (2017YFF0210200 and 2016YFD0201302) and the Agricultural Project for Public Technology Research in Zhejiang Province (2016C32004).

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Authors and Affiliations

  1. Institute of Pesticide and Environmental Toxicology, Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Zhejiang, 310058, Hangzhou, China

    Shasha Jiao, Pengyan Liu, Ying Liu, Rubing Zou, Ying Zhao, Yihua Liu, Guonian Zhu & Yirong Guo

  2. Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Zhejiang, 311400, Hangzhou, China

    Yihua Liu

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  1. Shasha Jiao
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  2. Pengyan Liu
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  3. Ying Liu
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  4. Rubing Zou
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  5. Ying Zhao
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  6. Yihua Liu
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  7. Guonian Zhu
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Corresponding authors

Correspondence to Yihua Liu or Yirong Guo.

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All studies on mice were performed under the guidance of the animal welfare committee of Zhejiang University in China.

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The authors have declared no conflict of interests.

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Jiao, S., Liu, P., Liu, Y. et al. Binding properties of broad-specific monoclonal antibodies against three organophosphorus pesticides by a direct surface plasmon resonance immunosensor. Anal Bioanal Chem 410, 7263–7273 (2018). https://doi.org/10.1007/s00216-018-1337-7

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  • DOI: https://doi.org/10.1007/s00216-018-1337-7

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