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Monitoring dynamic release of intracellular hydrogen peroxide through a microelectrode based enzymatic biosensor

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A Correction to this article was published on 05 July 2018

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Abstract

A high sensitive and selective hydrogen peroxide (H2O2) biosensor was fabricated on the basis of reduced hemoglobin (Hb) and single-walled carbon nanotubes (SWCNTs) for detecting the release of H2O2 from living HepG2 cancer cells in the process of the in situ biosynthesis of ZnO quantum. The modification of carbon fiber microelectrode (CFME) was carried out by physical adsorption. By the scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS), the dense cover of surface and successful immobilization were characterized. Electrochemical investigation demonstrates that the as-prepared modified microelectrode showed a quasi-reversible process toward the reduction of H2O2, which exhibited a linear range from 0.51 to 10.6 μM, with a limit of detection of 0.23 μM. This microelectrode biosensor was applied for the quantification of the change of H2O2 concentration released from HepG2 cells through the in situ biosynthesis of ZnO quantum dots, which was further confirmed by the fluorescence staining.

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  • 05 July 2018

    The authors would like to call the reader’s attention to the fact that unfortunately Alberto Pasquarelli’s and Kay-Eberhard Gottschalk’s affiliations were wrong in the original publication.

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Acknowledgements

This work is supported by the National High-tech R&D Program and National Key Research & Development Program of China (Nos. 2017YFA0205301), and the National Natural Science Foundation of China (Nos. 91753106, 81325011, 21327902).

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Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China

    Hang Zhang, Jun Ruan, Weiwei Liu, Xuerui Jiang, Tianyu Du, Hui Jiang & Xuemei Wang

  2. Institute of Experimental Physics, Ulm University, Albert Einstein Allee 45, 89069, Ulm, Germany

    Pasquarelli Alberto & Kay-Eberhard Gottschalk

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  1. Hang Zhang
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  2. Jun Ruan
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  3. Weiwei Liu
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  7. Pasquarelli Alberto
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Correspondence to Xuemei Wang.

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Zhang, H., Ruan, J., Liu, W. et al. Monitoring dynamic release of intracellular hydrogen peroxide through a microelectrode based enzymatic biosensor. Anal Bioanal Chem 410, 4509–4517 (2018). https://doi.org/10.1007/s00216-018-1108-5

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  • DOI: https://doi.org/10.1007/s00216-018-1108-5

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