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Lateral flow biosensor for multiplex detection of nitrofuran metabolites based on functionalized magnetic beads

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Abstract

The use of potential mutagenic nitrofuran antibiotic in food animal production has been banned world-wide. Common methods for nitrofuran detection involve complex extraction procedures. In the present study, magnetic beads functionalized with antibody against nitrofuran derivative were used as both the extraction and color developing media in lateral flow biosensor. Derivatization reagent carboxybenzaldehyde is firstly modified with ractopamine. After reaction with nitrofuran metabolites, the resultant molecule has two functional groups: the metabolite moiety and the ractopamine moiety. Metabolite moiety is captured by the antibody that is coated on magnetic beads. This duplex is then loaded onto biosensor and ractopamine moiety is further captured by the antibody immobilized on the test zone of nitrocellulose membrane. Without tedious organic reagent-based extraction procedure, this biosensor was capable of visually detecting four metabolites simultaneously with a detection limit of 0.1 μg/L. No cross-reactivity was observed in the presence of 50 μg/L interferential components.

Derivatization of nitrofuran metabolites (AHD, AOZ, SEM, or AMOZ) and LFA detection of the derivative products

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Acknowledgments

This work was financially supported by the Science & Technology Project in Guangzhou Zhujiang Science & Technology New Star (2014j2200080), Funds for Public Welfare Research in Guangdong Province (2014B020212011), and Strategic Cooperation Project of the Chinese Academy of Sciences and Guangdong Province (2012B091100267).

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Authors and Affiliations

  1. Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, 510530, China

    Xuewen Lu, Xiaoling Liang, Jianghong Dong, Zhiyuan Fang & Lingwen Zeng

  2. Institute of Environmental and Food Safety, Wuhan Academy of Agricultural Science and Technology, Wuhan, Hubei, 430000, China

    Xiaoling Liang & Lingwen Zeng

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  1. Xuewen Lu
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  2. Xiaoling Liang
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  3. Jianghong Dong
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  4. Zhiyuan Fang
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  5. Lingwen Zeng
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Correspondence to Zhiyuan Fang or Lingwen Zeng.

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Lu, X., Liang, X., Dong, J. et al. Lateral flow biosensor for multiplex detection of nitrofuran metabolites based on functionalized magnetic beads. Anal Bioanal Chem 408, 6703–6709 (2016). https://doi.org/10.1007/s00216-016-9787-2

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  • DOI: https://doi.org/10.1007/s00216-016-9787-2

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