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Tag-femtosecond laser-induced breakdown spectroscopy for the sensitive detection of cancer antigen 125 in blood plasma

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Abstract

Successful treatment of cancers requires detecting early signs of the disease. One promising way to approach this is to develop minimally invasive tests for the sensitive and specific detection of biomarkers in blood. Irrespective of the detection approach one uses, this remains a challenging task because biomarkers are typically present in low concentrations and there are signals that interfere strongly with prevailing compounds of human fluids. In this paper, we show that elemental encoded particle assay coupled with femtosecond laser-induced breakdown spectroscopy for simultaneous multi-elemental analysis can significantly improve biomarker detectability. An estimated near single molecule per particle efficiency of this method leads to sensitive detection of ovarian cancer biomarker CA125 in human blood plasma. This work opens new ways for earlier detection of cancers and for multiplex assay developments in various analytical applications from proteomics, genomics, and neurology fields.

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Acknowledgments

This research was supported by grants from the National Science Foundation Centers of Research Excellence in Science and Technology (0630388) and National Aeronautics and Space Administration (NX09AU90A). We thank the Blood Bank of Delmarva for providing human blood plasma for the research.

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Authors and Affiliations

  1. Optical Science Center for Applied Research and Applications and Department of Physics and Engineering, Delaware State University, Dover, DE, 19901, USA

    Yuri Markushin, Poopalasingam Sivakumar & Noureddine Melikechi

  2. Fox Chase Cancer Center, Philadelphia, PA, 19111, USA

    Denise Connolly

Authors
  1. Yuri Markushin
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  2. Poopalasingam Sivakumar
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  3. Denise Connolly
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  4. Noureddine Melikechi
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Correspondence to Noureddine Melikechi.

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Markushin, Y., Sivakumar, P., Connolly, D. et al. Tag-femtosecond laser-induced breakdown spectroscopy for the sensitive detection of cancer antigen 125 in blood plasma. Anal Bioanal Chem 407, 1849–1855 (2015). https://doi.org/10.1007/s00216-014-8433-0

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  • DOI: https://doi.org/10.1007/s00216-014-8433-0

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