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Novel polystyrene/antibody nanoparticle-coated capillary for immunoaffinity in-tube solid-phase microextraction

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An Erratum to this article was published on 10 May 2015

Abstract

Antibody-coated polystyrene (PS) nanoparticles (denoted as PS/IgG) were prepared and chemically immobilized for the first time onto a capillary inner wall for immunoaffinity in-tube solid-phase microextraction (SPME) of β2-microglobin (β2MG) and cystatin C (Cys-C). Scanning electron microscopy images of the prepared capillary showed that the nanoparticles were evenly coated onto the capillary inner surface, resulting in the undulating surface of the capillary inner wall. The extraction capacity of the nanoparticle-coated capillary was nearly five times higher than that of a monolayer antibody-immobilized capillary. The in-tube SPME recovery of β2MG (or Cys-C) by the nanoparticle-functionalized capillary was more than 97.8 %, whereas that by the monolayer antibody-immobilized tube was 30.5 %. In addition, the method quantitation limit obtained by using the nanoparticle-coated capillary was ten times lower than that by the monolayer capillary. Therefore, the capillary coated by PS/IgG nanoparticles is more suitable for immunoaffinity in-tube SPME compared with the commonly used monolayer antibody-immobilized capillary.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 31402889) and Natural Science Foundation of Tianjin (No. 14JCYBJC24300). We would like to thank Mr. Haifeng Zhao (Epsilon Bio. Tech. Co.) for his valuable discussion and suggestion.

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Authors and Affiliations

  1. Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin, 300070, China

    Bei Xu, Shuai Cheng, Xianhua Wang, Dongmei Wang & Liang Xu

  2. School of Public Health, Tianjin Medical University, Tianjin, 300070, China

    Bei Xu

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  1. Bei Xu
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  2. Shuai Cheng
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  3. Xianhua Wang
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  4. Dongmei Wang
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  5. Liang Xu
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Correspondence to Liang Xu.

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Xu, B., Cheng, S., Wang, X. et al. Novel polystyrene/antibody nanoparticle-coated capillary for immunoaffinity in-tube solid-phase microextraction. Anal Bioanal Chem 407, 2771–2775 (2015). https://doi.org/10.1007/s00216-014-8419-y

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  • DOI: https://doi.org/10.1007/s00216-014-8419-y

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