Abstract
A novel molecularly imprinted material based on silica microparticles was synthesized by surface polymerization with 3-chloro-1,2-propandiol (3-MCPD) as a template molecule. The molecularly imprinted polymer (MIP) was characterized by infrared spectroscopy and scanning electron microscopy. The adsorption of 3-MCPD by MIP was measured by gas chromatography with electron capture detection (GC-ECD) and an equilibrium binding experiment. Scatchard analysis revealed that the maximum apparent binding capacities of the MIP and non-imprinted polymer (NIP) were 67.64 and 23.31 μmol/g, respectively. The new adsorbent was successfully used in solid-phase extraction (SPE) to selectively enrich and determine 3-MCPD in soy sauce samples. The MIP-SPE column achieves recoveries higher than 92.7 % with a relative standard deviation of less than 1.83 %. The MIP-SPE-GC protocol improved the selectivity and eliminated the effects of template leakage on quantitative analysis and could be used for the determination of 3-MCPD in other complex food samples.
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Acknowledgments
The authors are mainly grateful to “973” National Basic Research Program of China (No. 2012CB720804), and the Commonweal Project of the Ministry of Agriculture (No. 201203069-1) for funding the research. This work has also been supported by Program for New Century Excellent Talents in Jiangsu University, Synergetic Innovation Center of Food Safety & Quality Control Jiangsu Province, the Priority Academic Program Development of Jiangsu Higher Education Institutions, and partly supported by “Risk Assessment for Agro-products Quality & Safety” Financial Fund of Ministry of Agriculture.
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Li, Y., Zheng, C., Sun, X. et al. Identification of 3-chloro-1,2-propandiol using molecularly imprinted composite solid-phase extraction materials. Anal Bioanal Chem 406, 6319–6327 (2014). https://doi.org/10.1007/s00216-014-8059-2
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DOI: https://doi.org/10.1007/s00216-014-8059-2