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Carbon nanotube wiring for signal amplification of electrochemical magneto immunosensors: application to myeloperoxidase detection

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Abstract

In this work, chronoamperometric myelo-peroxidase (MPO) detection was accomplished using immunofunctionalized magnetic microparticles (MPs), disposable carbon screen-printed electrodes (C-SPEs), and a ready-to-use commercially available tetramethylbenzidine (TMB)-based enzymatic substrate. In order to reach the limit of detection (LOD) needed to study real blood serum samples, assay performance was additionally improved by exploiting CNT wiring, which amplified the signal and decreased the LOD. The optimized assay can be performed in 30 min and yields LODs of 6 and 55 ng mL−1 in PBS and undiluted human serum, respectively, making it useful for the identification of patients at risk of cardiovascular disease. These results demonstrate that electrode nanostructuring can be accomplished “post-assay,” which favors the development of enhanced magneto immunosensors based on the exploitation of cheap and simple SPE devices.

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Acknowledgments

E.B. is supported by the Miguel Servet program, funded by the Fondo de Investigaciones Sanitarias of the Instituto de Salud Carlos III (CP13/00052).

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Authors and Affiliations

  1. IK4-Ikerlan Technological Research Centre, 20500, Mondragón, Spain

    Zorione Herrasti & Fernando Martínez

  2. Institut de Microelectrònica de Barcelona (IMB-CNM, CSIC), Esfera UAB, Campus Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain

    Eva Baldrich

  3. Diagnostic Nanotools, Molecular Biology and Biochemistry Research Center for Nanomedicine (Cibbim-Nanomedicine), Vall d’Hebron Institut de Recerca (VHIR), Universitat Autònoma de Barcelona, Passeig de la Vall d’Hebron 119-129, 08035, Barcelona, Spain

    Eva Baldrich

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  1. Zorione Herrasti
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  2. Fernando Martínez
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  3. Eva Baldrich
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Correspondence to Eva Baldrich.

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Herrasti, Z., Martínez, F. & Baldrich, E. Carbon nanotube wiring for signal amplification of electrochemical magneto immunosensors: application to myeloperoxidase detection. Anal Bioanal Chem 406, 5487–5493 (2014). https://doi.org/10.1007/s00216-014-7954-x

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  • DOI: https://doi.org/10.1007/s00216-014-7954-x

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