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A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system

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Abstract

The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83 ± 4.3 % (mean ± SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification.

Initially, the 3-amino-phenylboronic acid (APBA) reacted with QDs-COOH to prepare the PBA capped QDs (QDs-PBA). Then, the vesicular stomatitis virus (VSV), on which the surface of the envelope is abundant with sialic acid (SA), could be labeled efficiently and specifically with QDs-PBA in one step based on sialic acid-phenylboronic acid recognition system. The labeling efficiency attained to (83 ± 4.3 %) (mean ± SD). The labeled VSA (QDs-VSV) was still infectious and capable of being imaged at the single virion level

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 Program, no. 2011CB933600), the National Natural Science Foundation of China (No. 201372028) and the 863 program (SS2013AA031904).

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Authors and Affiliations

  1. School of Life Science, Beijing Institute of Technology, Beijing, 100081, China

    Li-Li Huang, Yong-Jie Jin, Dongxu Zhao, Chao Yu, Jian Hao & Hai-Yan Xie

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  1. Li-Li Huang
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  2. Yong-Jie Jin
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  3. Dongxu Zhao
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  4. Chao Yu
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  5. Jian Hao
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Correspondence to Hai-Yan Xie.

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Huang, LL., Jin, YJ., Zhao, D. et al. A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system. Anal Bioanal Chem 406, 2687–2693 (2014). https://doi.org/10.1007/s00216-014-7651-9

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  • DOI: https://doi.org/10.1007/s00216-014-7651-9

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