Abstract
Various chiral selectors have been utilized successfully in capillary electrophoresis (CE); however, the number of polysaccharides used as chiral selectors is still small and the mechanism of enantiorecognition has not been fully elucidated. Chondroitin sulfate D (CSD) and chondroitin sulfate E (CSE), belonging to the group of glycosaminoglycans, are linear, sulfated polysaccharides with large mass. In this paper, they were investigated for the first time for their potential as chiral selectors by CE. The effect of buffer composition and pH, chiral selector concentration, and applied voltage were systematically examined and optimized. A variety of drug enantiomers were resolved in the buffer pH range of 2.8–3.4 using 20 mM Tris/H3PO4 buffer with 5.0 % CSD or CSE and 20 kV applied voltage. A central composite design was used to validate the optimized separation parameters and satisfactory uniformity was obtained. As observed, CSE allowed satisfactory separation of the enantiomers of amlodipine, laudanosine, nefopam, sulconazole, and tryptophan methyl ester, as well as partial resolution of citalopram, duloxetine, and propranolol under the optimized conditions. CSD allowed partial or nearly baseline separation of amlodipine, laudanosine, nefopam, and sulconazole. The results indicated that CSE has a better enantiorecognition capability than CSD toward the tested drugs.
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References
Chankvetadze B (2007) Enantioseparations by using capillary electrophoretic techniques: the story of 20 and a few more years. J Chromatogr A 1168:45–70
Gübitz G, Schmid MG (2008) Chiral separation by capillary electromigration techniques. J Chromatogr A 1204:140–156
Gasparrini F, Misiti D, Villani C (2001) High-performance liquid chromatography chiral stationary phases based on low-molecular-mass selectors. J Chromatogr A 906:35–50
Schurig V (2001) Separation of enantiomers by gas chromatography. J Chromatogr A 906:275–299
Terfloth G (2001) Enantioseparations in super-and subcritical fluid chromatography. J Chromatogr A 906(2001):301–307
Chen J, Du Y, Zhu F, Chen B (2010) Evaluation of the enantioselectivity of glycogen-based dual chiral selector systems towards basic drugs in capillary electrophoresis. J Chromatogr A 1217:7158–7163
Chen B, Du Y (2010) Evaluation of the enantioseparation capability of the novel chiral selector clindamycin phosphate towards basic drugs by micellar electrokinetic chromatography. J Chromatogr A 1217:1806–1812
Du Y, Chen B (2010) Recent advances in enantioseparation by capillary electrophoresis using antibiotics and polysaccharides as chiral selectors. Chim Oggi 28:37–42
Chen B, Du Y, Wang H (2010) Study on enantiomeric separation of basic drugs by NACE in methanol-based medium using erythromycin lactobionate as a chiral selector. Electrophoresis 31:371–377
Yu T, Du Y, Chen B (2011) Evaluation of clarithromycin lactobionate as a novel chiral selector for enantiomeric separation of basic drugs in capillary electrophoresis. Electrophoresis 32:1898–1905
Hua X, Du Y, Chen J, Xu G, Yu T, Zhang Q (2013) Evaluation of the enantioselectivity of carbon nanoparticles modified chiral separation systems using dextrin as chiral selector by capillary EKC. Electrophoresis 34:1901–1907
Zhang Q, Du Y (2013) Evaluation of the enantioselectivity of glycogen-based synergistic system with amino acid chiral ionic liquids as additives in capillary electrophoresis. J Chromatogr A 1306:97–103
Fanali S (1989) Separation of optical isomers by capillary zone electrophoresis based on host-guest complexation with cyclodextrins. J Chromatogr 474:441–446
Terabe S (1989) Electrokinetic chromatography: an interface between electrophoresis and chromatography. Trends Anal Chem 8:129–134
Tait RJ, Thompson DO, Stella VJ, Stobaugh JF (1994) Sulfobutyl ether. beta.-cyclodextrin as a chiral discriminator for use with capillary electrophoresis. Anal Chem 66:4013–4018
Armstrong DW, Rundlett KL, Chen J (1994) Evaluation of the macrocyclic antibiotic vancomycin as a chiral selector for capillary electrophoresis. Chirality 6:496–509
Ward TJ, Dann IIIC, Blaylock A (1995) Enantiomeric resolution using the macrocyclic antibiotics rifamycin B and rifamycin SV as chiral selectors for capillary electrophoresis. J Chromatogr A 715:337–344
Ishihama Y, Terabe S (1993) Enantiomeric separation by micellar electrokinetic chromatography using saponins. J Liq Chromatogr 16:933–944
Mazzeo JP, Grover ER, Swartz ME, Petersen JS (1994) Novel chiral surfactant for the separation of enantiomers by micellar electrokinetic capillary chromatography. J Chromatogr A 680:125–135
Nishi H, Kuwahara Y (2001) Enantiomer separation by capillary electrophoresis utilizing noncyclic mono-, oligo-and polysaccharides as chiral selectors. J Biochem Biophys Methods 48:89–102
Wei W, Guo B, Lin JM (2009) Helical- and ahelical- dependent chiral recognition mechanisms in capillary electrophoresis using amylose as the selector. Electrophoresis 30:1380–1387
Chen J, Du Y, Zhu F, Chen B (2010) Glycogen: a novel branched polysaccharide chiral selector in CE. Electrophoresis 31:1044–1050
Zhu F, Du Y, Chen J, Chen B, Zhu Y, Zhai X, Xu S, Zhou W (2009) Enantioselective separation of basic drugs by CE with polygalacturonic acid as a novel chiral selector. Chromatographia 69:1315–1320
Gübitz G, Schmid MG (2007) Advances in chiral separation using capillary electromigration techniques. Electrophoresis 28:114–126
Nishi H, Izumoto S, Nakamura K, Nakai H, Sato T (1996) Dextran and dextrin as chiral selectors in capillary zone electrophoresis. Chromatographia 42:617–630
D’Hulst A, Verbeke N (1992) Chiral separation by capillary electrophoresis with oligosaccharides. J Chromatogr A 608:275–287
D’Hulst A, Verbeke N (1994) Quantitation in chiral capillary electrophoresis: theoretical and practical considerations. Electrophoresis 15:854–863
Nishi H (1996) Enantiomer separation of basic drugs by capillary electrophoresis using ionic and neutral polysaccharides as chiral selectors. J Chromatogr A 735:345–351
Watanabe T, Takahashi K, Horiuchi M, Kato K, Nakazawa H, Sugimoto T (1999) Chiral separation and quantitation of pentazocine enantiomers in pharmaceuticals by capillary zone electrophoresis using maltodextrins. J Pharm Biomed Anal 21:75–81
Stalcup AM, Agyel NM (1994) Heparin: a chiral mobile-phase additive for capillary zone electrophoresis. Anal Chem 66:3054–3059
Agyei NM, Gahm KH, Stalcup AM (1995) Chiral separations using heparin and dextran sulfate in capillary zone electrophoresis. Anal Chim Acta 307:185–191
Phinney KW, Sander LC (2003) Enantioselective separations in capillary electrophoresis with dextran sulfate as the chiral selector. Anal Bioanal Chem 375:763–768
Wang X, Lee J, Armstrong DW (1999) Separation of enantiomers by capillary electrophoresis using pentosan polysulfate. Electrophoresis 20:162–170
Volpi N (2009) Quality of different chondroitin sulfate preparations in relation to their therapeutic activity. J Pharm Pharmacol 61:1271–1280
Nishi H, Nakamura K, Nakai H, Sato T (1995) Enantiomeric separation of drugs by mucopolysaccharide-mediated electrokinetic chromatography. Anal Chem 67:2334–2341
Gotti R, Cavrini V, Andrisano V, Mascellani G (1998) Dermatan sulfate as useful chiral selector in capillary electrophoresis. J Chromatogr A 814:205–211
Tsukamoto T, Ushio T, Haginaka J (1999) Chiral resolution of basic drugs by capillary electrophoresis with new glycosaminoglycans. J Chromatogr A 864:163–171
Gotti R, Cavrini V, Andrisano V, Mascellani G (1999) Semisynthetic chondroitins as chiral buffer additives in capillary electrophoresis. J Chromatogr A 845:247–256
Acknowledgments
This work was supported by the Project of National Natural Science Foundation of China (No. 81001422) and the Fundamental Research Funds for the Central Universities (Program No. JKZ2011010).
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The authors have declared no conflict of interest.
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Zhang, Q., Du, Y., Chen, J. et al. Investigation of chondroitin sulfate D and chondroitin sulfate E as novel chiral selectors in capillary electrophoresis. Anal Bioanal Chem 406, 1557–1566 (2014). https://doi.org/10.1007/s00216-013-7544-3
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DOI: https://doi.org/10.1007/s00216-013-7544-3