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Online dynamic pH junction–sweeping for the determination of benzoic and sorbic acids in food products by capillary electrophoresis

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Abstract

A simple, low-cost, and efficient online focusing method that combines a dynamic pH junction and sweeping by capillary electrophoresis with polymer solutions was developed and optimized for the simultaneous determination of benzoic acid (BA) and sorbic acid (SA). A sample solution consisting of 2.5 mM phosphate at pH 3.0 and a buffer solution containing 15 mM tetraborate (pH 9.2), 40 mM sodium dodecyl sulfate, and 0.100 % (w/v) poly(ethylene oxide) were utilized to realize dynamic pH junction–sweeping for BA and SA. Under the optimized conditions, the entire analysis process was completed in 7 min, and a 900-fold sensitivity enhancement was achieved with limits of detection (S/N = 3) as low as 8.2 and 6.1 nM for BA and SA, respectively. The linear ranges were between 20 nM and 20 μM for BA and 20 nM and 10 μM for SA, with correlation coefficients greater than 0.992. The recoveries of the proposed method ranged from 90 to 113 %. These satisfactory results indicate that this method has the potential to be an effective analytical tool for the rapid screening of BA and SA in different food products.

An online focusing strategy combining dynamic pH junction and sweeping for sensitive determination of benzoic and sorbic acid in food products using capillary electrophoresis wit polymer solutions

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References

  1. Han P, Jia Z, Liu M, Li Y, Liu H, Yang H, Wang X, Ban F, Zhang S (2007) Electrophoresis 28:4114–4119

    Article  CAS  Google Scholar 

  2. Farahani H, Ganjali MR, Dinarvand R, Norouzi P (2009) J Agric Food Chem 57:2633–2639

    Article  CAS  Google Scholar 

  3. Lino CM, Pena A (2010) Food Chem 121:503–508

    Article  CAS  Google Scholar 

  4. Jangbai W, Wongwilai W, Grudpan K, Lapanantnoppakhun S (2012) Food Anal Methods 5:631–636

    Article  Google Scholar 

  5. Alizadeh N, Saburi N, Hosseini SE (2012) Food Control 28:315–320

    Article  CAS  Google Scholar 

  6. Berger TA, Berger BK (2013) Chromatographia 76:393–399

    Article  CAS  Google Scholar 

  7. Zengin N, Yüzbaşıoğlu D, Ünal F, Yılmaz S, Aksoy H (2011) Food Chem Toxicol 49:763–769

    Article  CAS  Google Scholar 

  8. Park H-W, Park EH, Yun H-M, Rhim H (2011) J Food Biochem 35:1034–1046

    Article  CAS  Google Scholar 

  9. Mamur S, Yüzbaşıoğlu D, Ünal F, Aksoy H (2012) Cytotechnology 64:553–562

    Article  CAS  Google Scholar 

  10. Jurado-Sánchez B, Ballesteros E, Gallego M (2011) Talanta 84:924–930

    Article  Google Scholar 

  11. Kokya TA, Farhadi K, Kalhori AA (2012) Food Anal Methods 5:351–358

    Article  Google Scholar 

  12. Sun Y, Wang X, Huang Y, Pan Z, Wang L (2013) J Sep Sci 36:2268–2276

    Article  CAS  Google Scholar 

  13. Mahboubifar M, Sobhani Z, Dehghanzadeh G, Javidnia K (2011) Food Anal Methods 4:150–154

    Article  Google Scholar 

  14. Can NO, Arli G, Lafci Y (2011) J Sep Sci 34:2214–2222

    CAS  Google Scholar 

  15. Kamankesh M, Mohammadi A, Tehrani ZM, Ferdowsi R, Hosseini H (2013) Talanta 109:46–51

    Article  CAS  Google Scholar 

  16. Han F, He Y-Z, Li L, Fu G-N, Xie H-Y, Gan W-E (2008) Anal Chim Acta 618:79–85

    Article  CAS  Google Scholar 

  17. Costa ACO, da Silva PL, Tavares MFM, Micke GA (2008) J Chromatogr A 1204:123–127

    Article  CAS  Google Scholar 

  18. Ling DS, Xie H-Y, He Y-Z, Gan W-E, Gao Y (2010) J Chromatogr A 1217:7807–7811

    Article  CAS  Google Scholar 

  19. Wang P, Ding X, Li Y, Yang Y (2012) J AOAC Int 95:1069–1073

    Article  CAS  Google Scholar 

  20. Xue Y, Chen N, Luo C, Wang X, Sun C (2013) Anal Methods 5:2391–2397

    Article  CAS  Google Scholar 

  21. Chen Y, Lü W, Chen X, Teng M (2012) Cent Eur J Chem 10:611–638

    Article  Google Scholar 

  22. Šlampová A, Malá Z, Pantůčková P, Gebauer P, Boček P (2013) Electrophoresis 34:3–18

    Article  Google Scholar 

  23. Breadmore MC, Shallan AI, Rabanes HR, Gstoettenmayr D, Keyon A, Gaspar ASA, Dawod M, Quirino JP (2013) Electrophoresis 34:29–54

    Article  CAS  Google Scholar 

  24. Chiu T-C (2013) Anal Bioanal Chem 405:7910–7930

    Google Scholar 

  25. Kazarian AA, Hilder EF, Breadmore MC (2011) J Sep Sci 34:2800–2821

    Article  CAS  Google Scholar 

  26. El Deeb S, Iriban MA, Gust R (2011) Electrophoresis 32:166–183

    Article  Google Scholar 

  27. Britz-McKibbin P, Otsuka K, Terabe S (2002) Anal Chem 74:3736–3743

    Article  CAS  Google Scholar 

  28. Yu L, Li SFY (2005) Electrophoresis 26:4360–4367

    Article  CAS  Google Scholar 

  29. Chen Y, Zhang L, Cai Z, Chen G (2011) Analyst 136:1852–1858

    Article  CAS  Google Scholar 

  30. Hsu C-H, Hu C-C, Chiu T-C (2012) J Sep Sci 35:1359–1364

    Article  CAS  Google Scholar 

  31. Chiu T-C, Chang H-T (2007) J Chromatogr A 1146:118–124

    Article  CAS  Google Scholar 

  32. Kao Y-Y, Liu K-T, Huang M-F, Chiu T-C, Chang H-T (2010) J Chromatogr A 1217:582–587

    Article  CAS  Google Scholar 

  33. Wu C-W, Lee J-Y, Hu C-C, Chiu T-C (2013) J Chin Chem Soc (in press)

  34. NIH. http://sis.nlm.nih.gov/chemical.html. Accessed 13 Nov 2013

  35. Horáková J, Petr J, Maier V, Znaleziona J, Staňová A, Marák J, Kaniansky D, Šervčík J (2007) J Chromatogr A 1155:193–198

    Article  Google Scholar 

  36. Zhang X, Xu S, Sun Y, Wang Y, Wang C (2011) Chromatographia 73:1217–1221

    Article  CAS  Google Scholar 

  37. Wei R, Li W, Yang L, Jiang Y, Xie T (2011) Talanta 83:1487–1490

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the National Science Council of Taiwan under contract no. NSC 102-2113-M-143-001.

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Authors and Affiliations

  1. Department of Applied Science, National Taitung University, 684, Section 1, Chunghua Road, Taitung, 95002, Taiwan

    Shih-Hong Hsu, Cho-Chun Hu & Tai-Chia Chiu

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  1. Shih-Hong Hsu
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  2. Cho-Chun Hu
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  3. Tai-Chia Chiu
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Correspondence to Tai-Chia Chiu.

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Hsu, SH., Hu, CC. & Chiu, TC. Online dynamic pH junction–sweeping for the determination of benzoic and sorbic acids in food products by capillary electrophoresis. Anal Bioanal Chem 406, 635–641 (2014). https://doi.org/10.1007/s00216-013-7481-1

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  • DOI: https://doi.org/10.1007/s00216-013-7481-1

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