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An innovative strategy for direct electrochemical detection of microRNA biomarkers

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Abstract

We report an electrochemical method for direct, reagentless, and label-free detection of microRNA, based on a conjugated copolymer, poly(5-hydroxy-1,4-naphthoquinone-co-5-hydroxy-2-carboxyethyl-1,4-naphthoquinone), acting as hybridization transducer. Hybridization between the oligonucleotide capture probe and a microRNA target of 22 base pairs generates an increase in the redox current (“signal-on”), which is evidenced by square wave voltammetry. Selectivity is good, with little hybridization for non-complementary targets, and the limit of detection reaches 650 fM. It is also evidenced that this sensitivity benefits from the high affinity of DNA for RNA.

The biosensor gives a current increase (signal-on) upon miRNA addition. It was shown that miRNAs give better sensitivity than corresponding DNAs.

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Acknowledgments

H.V. Tran thanks the University of Sciences and Technology of Hanoi for a Ph.D. grant. The authors thank the University Paris Diderot for financial support through an interdisciplinary grant between the chemistry and odontology departments.

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Authors and Affiliations

  1. Univ. Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR 7086 CNRS, 15 rue J-A de Baïf, 75205, Paris Cedex 13, France

    H. V. Tran, B. Piro, S. Reisberg, G. Anquetin & M. C. Pham

  2. University of Science and Technology of Hanoi, 18 Hoang Quoc Viet, Hanoi, Vietnam

    H. V. Tran

  3. Université Paris XI, INSERM U-1014, Groupe Hospitalier Paul Brousse, 94800, Villejuif, France

    H. T. Duc

Authors
  1. H. V. Tran
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  2. B. Piro
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  3. S. Reisberg
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  4. G. Anquetin
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  5. H. T. Duc
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  6. M. C. Pham
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Corresponding author

Correspondence to B. Piro.

Additional information

Published in the special issue Analytical Science in France with guest editors Christian Rolando and Philippe Garrigues.

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Tran, H.V., Piro, B., Reisberg, S. et al. An innovative strategy for direct electrochemical detection of microRNA biomarkers. Anal Bioanal Chem 406, 1241–1244 (2014). https://doi.org/10.1007/s00216-013-7292-4

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  • DOI: https://doi.org/10.1007/s00216-013-7292-4

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