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Optimization in multidimensional gas chromatography applying quantitative analysis via a stable isotope dilution assay

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Abstract

Trace level analyses in complex matrices benefit from heart-cut multidimensional gas chromatographic (MDGC) separations and quantification via a stable isotope dilution assay. Minimization of the potential transfer of co-eluting matrix compounds from the first dimension (1D) separation into the second dimension separation requests narrow cut-windows. Knowledge about the nature of the isotope effect in the separation of labeled and unlabeled compounds allows choosing conditions resulting in at best a co-elution situation in the 1D separation. Since the isotope effect strongly depends on the interactions of the analytes with the stationary phase, an appropriate separation column polarity is mandatory for an isotopic co-elution. With 3-alkyl-2-methoxypyrazines and an ionic liquid stationary phase as an example, optimization of the MDGC method is demonstrated and critical aspects of narrow cut-window definition are discussed.

Avoiding chromatographic separation of isotopic standards by fine-tuning the isotope effect allows narrow cut-windows in SIDA based MDGC applications.

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References

  1. Marriott PJ, Chin S-T, Maikhunthod B, Schmarr H-G, Bieri S (2012) Multidimensional gas chromatography. TrAC, Trends Anal Chem 34:1–21

    Article  CAS  Google Scholar 

  2. Rychlik M, Asam S (2009) Stabilisotopenverdünnungsanalysen zur Quantifizierung organischer Spurenkomponenten in der Lebensmittelanalytik. Umweltwiss Schadst-Forsch 21(5):470–482

    Article  CAS  Google Scholar 

  3. Schmarr H-G, Slabizki P, Müntnich S, Metzger C, Gracia-Moreno E (2012) Ionic liquids as novel stationary phases in gas liquid chromatography: inverse or normal isotope effect? J Chromatogr A 1270:310–317

    Article  CAS  Google Scholar 

  4. Schmarr H-G, Sang W, Ganß S, Koschinski S, Meusinger R (2011) New insights into the synthesis and characterization of 2-methoxy-3-alkylpyrazines and their deuterated isotopologues. J Labelled Compd Radiopharm 54(8):438–440

    Article  CAS  Google Scholar 

  5. Schmarr H-G, Ganß S, Sang W, Potouridis T (2007) Analysis of 2-aminoacetophenone in wine using a stable isotope dilution assay and multidimensional gas chromatography-mass spectrometry. J Chromatogr A 1150(1–2):78–84

    CAS  Google Scholar 

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Acknowledgments

The authors are particularly grateful to Supelco (Sigma-Aldrich; SILEP program) for the ionic liquid separation columns evaluated in this work. C. Legrum is grateful to the “Graduiertenförderung des Landes Rheinland-Pfalz” for a Ph.D. scholarship. Also, financial support from the Ministerium für Umwelt, Landwirtschaft, Ernährung, Weinbau und Forsten (Rheinland-Pfalz, Germany) is gratefully acknowledged.

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Authors and Affiliations

  1. Dienstleistungszentrum Ländlicher Raum Rheinpfalz, Kompetenzzentrum Weinforschung, Breitenweg 71, 67435, Neustadt an der Weinstraße, Germany

    Hans-Georg Schmarr, Petra Slabizki & Charlotte Legrum

  2. Fachbereich Chemie, Fachrichtung Lebensmittelchemie und Toxikologie, Technische Universität Kaiserslautern, Erwin-Schrödinger-Straße 52, 67663, Kaiserslautern, Germany

    Charlotte Legrum

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  1. Hans-Georg Schmarr
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  2. Petra Slabizki
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  3. Charlotte Legrum
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Correspondence to Hans-Georg Schmarr.

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Hans-Georg Schmarr and Charlotte Legrum contributed equally to this work.

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Schmarr, HG., Slabizki, P. & Legrum, C. Optimization in multidimensional gas chromatography applying quantitative analysis via a stable isotope dilution assay. Anal Bioanal Chem 405, 6589–6593 (2013). https://doi.org/10.1007/s00216-013-7072-1

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  • DOI: https://doi.org/10.1007/s00216-013-7072-1

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