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Ion mobility spectrometry focusing on speciation analysis of metals/metalloids bound to carbonic anhydrase

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Abstract

In the present work, traveling wave ion mobility spectrometry-mass spectrometry (TWIMS-MS) was applied to speciation analysis of metalloproteins. The influence of pH on complexation conditions between some metals and bovine carbonic anhydrase was evaluated from pH 6 to 9, as well as the time involved in their complexation (0–24 h). Employing TWIMS-MS, two conformational states of bovine carbonic anhydrase were observed with charge states of +12 and +11; these configurations being evaluated in terms of the folded state of the apo form and this protein (at charge state +11) being linked to barium, lead, copper, and zinc in their divalent forms. Metalloprotein speciation analysis was carried out for copper (Cu+ and Cu2+), lead (Pb2+ and Pb4+), and selenium (Se4+ and Se6+) species complexed with bovine carbonic anhydrase. Mobilities of all complexed species were compared, also considering the apo form of this protein.

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Acknowledgments

The authors thank the Fundação de Amparo a Pesquisa do Estado de São Paulo (São Paulo, Brazil) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasília, Brazil) for financial support and fellowships. We are also grateful to Prof. Carol H. Collins for language assistance and Alexandre Ferreira Gomes for helping the authors with some experiments.

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Authors and Affiliations

  1. Spectrometry, Sample Preparation and Mechanization Group, GEPAM, Institute of Chemistry, University of Campinas—UNICAMP, P.O. Box 6154, Campinas, SP, 13083-970, Brazil

    Gustavo de Souza Pessôa & Marco Aurélio Zezzi Arruda

  2. National Institute of Science and Technology for Bioanalytics, Institute of Chemistry, University of Campinas—UNICAMP, PO Box 6154, 13084-862, Campinas, SP, Brazil

    Gustavo de Souza Pessôa, Eduardo Jorge Pilau, Fábio Cesar Gozzo & Marco Aurélio Zezzi Arruda

  3. Dalton Mass Spectrometry Group, Institute of Chemistry, University of Campinas—UNICAMP, PO Box 6154, 13084-862, Campinas, SP, Brazil

    Eduardo Jorge Pilau & Fábio Cesar Gozzo

Authors
  1. Gustavo de Souza Pessôa
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  2. Eduardo Jorge Pilau
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  3. Fábio Cesar Gozzo
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  4. Marco Aurélio Zezzi Arruda
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Corresponding author

Correspondence to Marco Aurélio Zezzi Arruda.

Additional information

Published in the topical collection (Bio)Analytical Research in Latin America with guest editors Marco A. Zezzi Arruda and Lauro Kubota.

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Pessôa, G.S., Pilau, E.J., Gozzo, F.C. et al. Ion mobility spectrometry focusing on speciation analysis of metals/metalloids bound to carbonic anhydrase. Anal Bioanal Chem 405, 7653–7660 (2013). https://doi.org/10.1007/s00216-013-7064-1

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  • DOI: https://doi.org/10.1007/s00216-013-7064-1

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