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Preparation of a butyl–silica hybrid monolithic column with a “one-pot” process for bioseparation by capillary liquid chromatography

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Abstract

A butyl–silica hybrid monolithic column for bioseparation by capillary liquid chromatography (cLC) was prepared with butyl methacrylate and alkoxysilanes through a “one-pot” process. The effects of polycondensation temperature, volume percentage of N,N′-dimethylformamide, and content of cetyltrimethylammonium bromide and butyl methacrylate on the morphologies of the hybrid monolithic columns prepared were investigated in detail. Baseline separations of proteins and small peptides on the hybrid monolithic column were achieved by cLC with gradient elution. In addition, the resulting hybrid column was also applied for analysis of tryptic digests of bovine serum albumin by cLC coupled with tandem mass spectrometry. The results demonstrate its potential application in separation of complex biological samples.

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References

  1. Wolters DA, Washburn MP, Yates JR (2001) An automated multidimensional protein identification technology for shotgun proteomics. Anal Chem 73:5683–5690

    Article  CAS  Google Scholar 

  2. Kennedy RT, Jorgenson JW (1989) Preparation and evaluation of packed capillary liquid chromatography columns with inner diameters from 20 to 50 micrometers. Anal Chem 61:1128–1135

    Article  CAS  Google Scholar 

  3. Nakanishi K, Minakuchi H, Soga N, Tanaka N (1997) Double pore silica gel monolith applied to liquid chromatography. J Sol-Gel Sci Technol 8:547–552

    CAS  Google Scholar 

  4. Svec F, Fréchet JMJ (1996) New designs of macroporous polymers and supports: from separation to biocatalysis. Science 273:205–211

    Article  CAS  Google Scholar 

  5. Xu Y, Cao Q, Svec F, Frechet JMJ (2010) Porous polymer monolithic column with surface-bound gold nanoparticles for the capture and separation of cysteine-containing peptides. Anal Chem 82:3352–3358

    Article  CAS  Google Scholar 

  6. Svec F (2009) My favorite materials: porous polymer monoliths. J Sep Sci 32:3–9

    Article  CAS  Google Scholar 

  7. Hayes JD, Malik A (2000) Sol-gel monolithic columns with reversed electroosmotic flow for capillary electrochromatography. Anal Chem 72:4090–4099

    Article  CAS  Google Scholar 

  8. Yan LJ, Zhang QH, Feng YQ, Zhang WB, Li T, Zhang LH, Zhang YK (2006) Octyl-functionalized hybrid silica monolithic column for reversed-phase capillary electrochromatography. J Chromatogr A1121:92–98

    Google Scholar 

  9. Minakuchi H, Nakanishi K, Soga N, Ishizuka N, Tanaka N (1996) Octadecylsilylated porous silica rods as separation media for reversed-phase liquid chromatography. Anal Chem 68:3498–3501

    Article  CAS  Google Scholar 

  10. Dong J, Xie CH, Tian RJ, Wu RA, Hu JW, Zou HF (2005) Capillary electrochromatography with a neutral monolithic column for classification of analytes and determination of basic drugs in human serum. Electrophoresis 26:3452–3459

    Article  CAS  Google Scholar 

  11. Sun XL, He XW, Chen LX, Zhang YK (2011) In-column “click” preparation of hydrophobic organic monolithic stationary phases for protein separation. Anal Bioanal Chem 399:3407–3413

    Article  CAS  Google Scholar 

  12. Hemstrom P, Nordborg A, Irgum K, Svec F, Frechet JMJ (2006) Polymer-based monolithic microcolumns for hydrophobic interaction chromatography of proteins. J Sep Sci 29:25–32

    Article  Google Scholar 

  13. Mant CT, Hodges RS (eds) (1991) High-performance liquid chromatography of peptides and proteins: separation, analysis, and conformation. CRC, Boca Raton

    Google Scholar 

  14. Lee D, Svec F, Fréchet JMJ (2004) Photopolymerized monolithic capillary columns for rapid micro high-performance liquid chromatographic separation of proteins. J Chromatogr A 1051:53–60

    Article  CAS  Google Scholar 

  15. Wu MH, Wu RA, Zhang ZB, Zou HF (2011) Preparation and application of organic-silica hybrid monolithic capillary columns. Electrophoresis 32:105–115

    Article  Google Scholar 

  16. Wu MH, Wu RA, Wang FJ, Ren LB, Dong J, Liu Z, Zou HF (2009) “One-pot” process for fabrication of organic-silica hybrid monolithic capillary columns using organic monomer and alkoxysilane. Anal Chem 81:3529–3536

    Article  CAS  Google Scholar 

  17. Zhang ZB, Wu MH, Wu RA, Dong J, Ou JJ, Zou HF (2011) Preparation of perphenylcarbamoylated β-cyclodextrin-silica hybrid monolithic column with “one-pot” approach for enantioseparation by capillary liquid chromatography. Anal Chem 83:3616–3622

    Article  CAS  Google Scholar 

  18. Zhang ZB, Lin H, Ou JJ, Qin HQ, Wu RA, Dong J, Zou HF (2012) Preparation of phenyl-silica hybrid monolithic column with “one-pot” process for capillary liquid chromatography. J Chromatogr A 1228:263–269

    Article  CAS  Google Scholar 

  19. Xie CH, Ye ML, Jiang XG, Jin WH, Zou HF (2006) Octadecylated silica monolith capillary column with integrated nanoelectrospray ionization emitter for highly efficient proteome analysis. Mol Cell Proteomics 5:454–461

    CAS  Google Scholar 

  20. Stanelle RD, Sander LC, Marcus RK (2005) Hydrodynamic flow in capillary-channel fiber columns for liquid chromatography. J Chromatogr A 1100:68–75

    Article  CAS  Google Scholar 

Download references

Acknowledgments

Financial support from the National Natural Sciences Foundation of China (nos. 20975101, 21105101) and the Creative Research Group Project by NSFC (no. 21021004) to H.Z. is greatly acknowledged.

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Authors and Affiliations

  1. CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R & A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian, 116023, China

    Zhenbin Zhang, Fangjun Wang, Junjie Ou, Hui Lin, Jing Dong & Hanfa Zou

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Zhenbin Zhang & Hui Lin

Authors
  1. Zhenbin Zhang
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  2. Fangjun Wang
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  3. Junjie Ou
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  4. Hui Lin
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  5. Jing Dong
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  6. Hanfa Zou
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Corresponding authors

Correspondence to Junjie Ou or Hanfa Zou.

Additional information

Published in the topical collection Monolithic Columns in Liquid Phase Separations with guest editor Luis A. Colon.

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Zhang, Z., Wang, F., Ou, J. et al. Preparation of a butyl–silica hybrid monolithic column with a “one-pot” process for bioseparation by capillary liquid chromatography. Anal Bioanal Chem 405, 2265–2271 (2013). https://doi.org/10.1007/s00216-012-6589-z

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  • DOI: https://doi.org/10.1007/s00216-012-6589-z

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