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Applications of stable isotope dimethyl labeling in quantitative proteomics

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Abstract

Mass spectrometry has proven to be an indispensable tool for protein identification, characterization, and quantification. Among the possible methods in quantitative proteomics, stable isotope labeling by using reductive dimethylation has emerged as a cost-effective, simple, but powerful method able to compete at any level with the present alternatives. In this review, we briefly introduce experimental and software methods for proteome analysis using dimethyl labeling and provide a comprehensive overview of reported applications in the analysis of (1) differential protein expression, (2) posttranslational modifications, and (3) protein interactions.

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Acknowledgments

We kindly acknowledge all members of the Heck group for their contributions. This work was in part supported by the PRIME-XS project (grant agreement number 262067), funded by the European Union Seventh Framework Programme, the Netherlands Proteomics Centre, embedded in the Netherlands Genomics Initiative, the Centre for Biomedical Genetics, the Netherlands Organization for Scientific Research (NWO) with a VIDI grant (700.10.429), and the Netherlands Bioinformatics Centre. D.K. is funded by Utrecht Institute for Pharmaceutical Sciences (UIPS) and a Royal Thai Scholarship.

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Authors and Affiliations

  1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands

    Duangnapa Kovanich, Salvatore Cappadona, Reinout Raijmakers, Shabaz Mohammed, Arjen Scholten & Albert J. R. Heck

  2. Netherlands Proteomics Centre, Padualaan 8, 3584 CH, Utrecht, The Netherlands

    Duangnapa Kovanich, Salvatore Cappadona, Reinout Raijmakers, Shabaz Mohammed, Arjen Scholten & Albert J. R. Heck

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  1. Duangnapa Kovanich
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  2. Salvatore Cappadona
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  3. Reinout Raijmakers
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  4. Shabaz Mohammed
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  5. Arjen Scholten
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  6. Albert J. R. Heck
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Correspondence to Albert J. R. Heck.

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Published in the topical issue Quantitative Mass Spectrometry in Proteomics with guest editors Bernhard Kuster and Marcus Bantscheff.

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Kovanich, D., Cappadona, S., Raijmakers, R. et al. Applications of stable isotope dimethyl labeling in quantitative proteomics. Anal Bioanal Chem 404, 991–1009 (2012). https://doi.org/10.1007/s00216-012-6070-z

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  • DOI: https://doi.org/10.1007/s00216-012-6070-z

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