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Comprehensive two-dimensional gas chromatography in metabolomics

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Abstract

One of the major objectives in metabolomics is the identification of subtle changes in metabolite profiles as affected by genetic or environmental factors. Comprehensive two-dimensional gas chromatography (GC × GC) hyphenated to a fast-acquisition mass spectrometer is a well-established analytical technique to study the composition of complex samples due to its enhanced separation capacity, sensitivity, peak resolution, and reproducibility. This review reports applications of GC × GC to metabolomics studies of sample of different types (biofluid, cells, tissue, bacteria, yeast, plants), and discusses its advantages and limitations.

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Acknowledgments

This project was funded in part by BayGene, the intramural ReForM program of the Faculty of Medicine at the University of Regensburg, and the DFG (KFO 262, DE 835/2-1).

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  1. Institute of Functional Genomics, University of Regensburg, Josef-Engert-Str. 9, 93053, Regensburg, Germany

    Martin F. Almstetter, Peter J. Oefner & Katja Dettmer

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  1. Martin F. Almstetter
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  2. Peter J. Oefner
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  3. Katja Dettmer
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Correspondence to Katja Dettmer.

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Published in the special issue Comprehensive Multidimensional Separations with guest editors James Harynuk and Philip Marriott.

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Almstetter, M.F., Oefner, P.J. & Dettmer, K. Comprehensive two-dimensional gas chromatography in metabolomics. Anal Bioanal Chem 402, 1993–2013 (2012). https://doi.org/10.1007/s00216-011-5630-y

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