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Titanium release in serum of patients with different bone fixation implants and its interaction with serum biomolecules at physiological levels

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Abstract

Increased concentrations of circulating metal-degradation products derived from the use of Ti orthopaedic implants may have deleterious biological effects over the long term. Therefore, there is an increasing need to establish the basal level of Ti in the serum of the population (exposed and non-exposed) with appropriate highly sensitive techniques and strategies. With this aim, we have developed a quantitative strategy for the determination of total Ti concentration in human serum samples by isotope dilution analysis using a double-focussing inductively coupled plasma mass spectrometer. Minimizing sample handling and therefore contamination issues, we obtained detection limits of about 0.05 μg L−1 Ti working at medium resolution (m/Δm 4000). Such extremely good sensitivity permitted us to establish the range of Ti concentration in serum of 40 control individuals (mean 0.26 μg L−1) and also to compare it with the level in exposed patients with different Ti metal implants. On the other hand, Ti transport “in vivo” studies have been enabled by online coupling of liquid chromatography (anion-exchange) separation and double-focussing inductively coupled plasma mass spectrometry for sensitive detection of Ti. The development of a postcolumn isotope dilution strategy permitted quantitative characterization of the Ti-transporting biomolecules in human serum. The results for unspiked serum revealed that 99.8% of the Ti present in this fluid is bound to the protein transferrin, with column recoveries greater than 95%.

This work illustrates the quantitative evaluation of the Ti released to serum due to shedding of the Ti containing implants by the blood components and its association to different serum biomolecules.

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References

  1. Nicholson JW (2002) The chemistry of medical and dental materials. RSC monographs. Royal Society of Chemistry, Cambridge

    Google Scholar 

  2. Lascombes P, Haumont T, Journeau P (2006) J Ped Orthop 26:827–834

    Article  Google Scholar 

  3. Jacobs JJ, Skipor AK, Urban RM, Galante JO (1991) J Bone Joint Surg (Am) 73:1475–1486

    CAS  Google Scholar 

  4. Wang JC, Yu WD, Sandhu HS, Betts F, Bhuta S, Delamarter RB (1999) Spine 24:899–903

    Article  CAS  Google Scholar 

  5. Hjalmarsson L, Smedberg JI, Wennerberg A (2011) J Oral Rehab 38:61–71

    Article  CAS  Google Scholar 

  6. Keegan GM, Learmonth ID, Case CP (2007) J Bone Joint Surg (Br) 89B:567–573

    Google Scholar 

  7. Brien WW, Salvati EA, Betts F, Bullough P, Wright T, Rimnac C, Buly R, Garvin K (1992) Clin Orthop 276:66–74

    Google Scholar 

  8. Jacobs JJ, Skipor AK, Campbell PA, Hallab NJ, Urban RM, Amstutz HC (2004) J Arthroplasty 19:S59–S65

    Article  Google Scholar 

  9. Dustan E, Sanghrajka AP, Tilley S, Unwin P, Blunn G, Cannon SR, Briggs TWR (2005) J Bone Joint Surg (Br) 87:628–631

    Article  Google Scholar 

  10. Chen J, Dong X, Zhao J, Tang G (2009) J App Toxicol 29:330–337

    Article  CAS  Google Scholar 

  11. Tinoco AD, Earnes EV, Valentine AM (2008) J Am Chem Soc 130:2262–2270

    Article  CAS  Google Scholar 

  12. Sarmiento-González A, Marchante-Gayón JM, Tejerina-Lobo JM, Paz Jiménez J, Sanz-Medel A (2008) Anal Bioanal Chem 391:2583–2589

    Article  Google Scholar 

  13. Richardson TD, Pineda SJ, Strenge KB, Van Fleet TA, McGregor M, Milbrandt JC, Espinosa JA, Freitag P (2008) Spine 33:792–796

    Article  Google Scholar 

  14. Tinoco AD, Valentine AM (2005) J Am Chem Soc 127:11218–11219

    Article  CAS  Google Scholar 

  15. Guo M, Sun H, McArdle HJ, Gambling L, Sadler PJ (2000) Biochemistry 39:10023–10033

    Article  CAS  Google Scholar 

  16. Sariego Muñiz C, Marchante Gayón JM, García Alonso JI, Sanz-Medel A (1999) J Anal At Spectrom 14:193–198

    Article  Google Scholar 

  17. Rottmann L, Heumann KG (1994) Fresenius J Anal Chem 350:221–227

    Article  CAS  Google Scholar 

  18. McGarry S, Morgan SJ, Grosskreuz RM, Williams AE, Smith WR (2008) J Trauma 64:430–433

    Article  CAS  Google Scholar 

  19. Patton MS, Lyon TDB, Ashcroft GP (2008) Acta Orthop 79:820–825

    Article  Google Scholar 

  20. Nuevo-Ordóñez Y, Montes-Bayón M, Blanco-González E, Paz-Jiménez J, Tejerina-Lobo JM, Pena-López M, Sanz-Medel A (2009) J Anal At Spectrom 24:1037–1043

    Article  Google Scholar 

  21. Sarmiento A, Marchante Gayon J, Ruiz Encinar J, Sanz-Medel A (2008) Anal Chem 80:8702–8711

    Article  Google Scholar 

  22. Del Castillo ME, Montes-Bayón M, Sanz-Medel A (2006) Anal Chem 82:8218–8226

    Google Scholar 

Download references

Acknowledgements

The authors gratefully acknowledge the Regional Government of Asturias (FICYT) for financial support through the projects PC06-035 and PC10-27.

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Authors and Affiliations

  1. Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, C/Julian Clavería 8, 33006, Oviedo, Spain

    Yoana Nuevo-Ordóñez, M. Montes-Bayón, E. Blanco-González & A. Sanz-Medel

  2. Department of Surgery, Faculty of Medicine, University of Oviedo, C/ Julián Clavería 6, 33006, Oviedo, Spain

    J. Paz-Aparicio & J. Diánez Raimundez

  3. Department of Stomatology, School of Stomatology, University of Oviedo, C/ Catedrático José Serrano s/n, 33009, Oviedo, Spain

    J. M. Tejerina & M. A. Peña

Authors
  1. Yoana Nuevo-Ordóñez
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  2. M. Montes-Bayón
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  3. E. Blanco-González
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  4. J. Paz-Aparicio
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  5. J. Diánez Raimundez
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  6. J. M. Tejerina
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  7. M. A. Peña
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  8. A. Sanz-Medel
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Corresponding authors

Correspondence to M. Montes-Bayón or A. Sanz-Medel.

Additional information

Published in the special issue Plasma Spectrochemistry with guest editors Juan Castillo and Martín Resano.

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Nuevo-Ordóñez, Y., Montes-Bayón, M., Blanco-González, E. et al. Titanium release in serum of patients with different bone fixation implants and its interaction with serum biomolecules at physiological levels. Anal Bioanal Chem 401, 2747–2754 (2011). https://doi.org/10.1007/s00216-011-5232-8

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  • DOI: https://doi.org/10.1007/s00216-011-5232-8

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