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Simultaneous determination of disulfiram and bupropion in human plasma of alcohol and nicotine abusers

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Abstract

An isocratic high-performance liquid-chromatographic method has been developed for the simultaneous determination of disulfiram and bupropion in human plasma samples. Analyses were carried out on a C8 reversed-phase column using a mobile phase composed of 50% acetonitrile and 50% aqueous phosphate buffer, containing triethylamine. Diode-array detection was used, operating at a wavelength of 250 nm. For the clean-up of plasma samples, a solid phase extraction procedure, based on C2 cartridges, was implemented. Extraction yields of the analytes were satisfactory, being always higher than 84%. The calibration curve was linear over the 5–500 ng mL-1 plasma concentration range for both disulfiram and bupropion. The method showed a high sensitivity (limit of detection of 1.5 ng mL-1) and satisfactory precision, selectivity and accuracy. The application to human plasma samples obtained from some alcohol and nicotine abusers also gave good results.

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Acknowledgements

Thanks are due to Sara Bramucci for technical assistance. This study was supported by grants from MIUR (Ministero dell'Istruzione, dell'Università e della Ricerca, Italy): RFO (ex-60%) funds.

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Authors and Affiliations

  1. Department of Pharmaceutical Sciences, University of Bologna, Via Belmeloro 6, 40126, Bologna, Italy

    Maria Addolorata Saracino, Chiara Marcheselli, Francesco De Stefano & Maria Augusta Raggi

  2. Addiction Treatment Centre, Health Local Unit ASL, Via Pier Maffei 59, 13836, Cossato, Biella, Italy

    Lorenzo Somaini

  3. United Nations Office on Drugs and Crime, Global Challenges Section, Division for Operations, P.O. Box 500, 1400, Vienna, Austria

    Gilberto Gerra

  4. Addiction Treatment Centre, Health Local Unit ASL, Via Carpaccio 2, 40138, Bologna, Italy

    Maria Chiara Pieri

  5. Laboratory of Pharmaco-Toxicological Analysis, Via Belmeloro 6, 40126, Bologna, Italy

    Maria Augusta Raggi

Authors
  1. Maria Addolorata Saracino
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  2. Chiara Marcheselli
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  3. Lorenzo Somaini
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  4. Gilberto Gerra
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  5. Francesco De Stefano
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  6. Maria Chiara Pieri
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  7. Maria Augusta Raggi
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Correspondence to Maria Augusta Raggi.

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Saracino, M.A., Marcheselli, C., Somaini, L. et al. Simultaneous determination of disulfiram and bupropion in human plasma of alcohol and nicotine abusers. Anal Bioanal Chem 398, 2155–2161 (2010). https://doi.org/10.1007/s00216-010-4172-z

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  • DOI: https://doi.org/10.1007/s00216-010-4172-z

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